Skip to main content
PMC home page
Biochemical Journal logoLink to Biochemical Journal
. 1992 Apr 15;283(Pt 2):575–581. doi: 10.1042/bj2830575

Acute effects of cholestatic and choleretic bile salts on vasopressin- and glucagon-induced hepato-biliary calcium fluxes in the perfused rat liver.

Y Hamada 1, A Karjalainen 1, B A Setchell 1, J E Millard 1, F L Bygrave 1
PMCID: PMC1131074  PMID: 1575700

Abstract

The effects were investigated of the choleretic bile salt glycoursodeoxycholate (G-UDCA) and of the cholestatic bile salt taurochenodeoxycholate (T-CDCA) on changes in perfusate Ca2+, glucose and oxygen and in bile calcium and bile flow induced by the administration of (a) vasopressin, (b) glucagon and (c) glucagon plus vasopressin together to the perfused rat liver [Hamada, Karjalainen, Setchell, Millard & Bygrave (1992) Biochem. J. 281, 387-392]. G-UDCA itself increased the secretion of calcium in the bile several-fold, but its principal effect was to augment each of the above-mentioned metabolic events except glucose and oxygen output; particularly noteworthy was its ability to augment the 'transients' in bile calcium and bile flow seen immediately after the administration of vasopressin with or without glucagon. T-CDCA, by contrast, produced opposite effects and attenuated all of the parameters measured, and in particular the transients in bile calcium and bile flow. The data provide evidence of a strong correlation between calcium fluxes occurring on both the sinusoidal and the bile-canalicular membranes and that all are modifiable by glucagon, Ca(2+)-mobilizing hormones and bile salts.

Full text

PDF
575

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Altin J. G., Bygrave F. L. Second messengers and the regulation of Ca2+ fluxes by Ca2+-mobilizing agonists in rat liver. Biol Rev Camb Philos Soc. 1988 Nov;63(4):551–611. doi: 10.1111/j.1469-185x.1988.tb00670.x. [DOI] [PubMed] [Google Scholar]
  2. Altin J. G., Bygrave F. L. Synergistic stimulation of Ca2+ uptake by glucagon and Ca2+-mobilizing hormones in the perfused rat liver. A role for mitochondria in long-term Ca2+ homoeostasis. Biochem J. 1986 Sep 15;238(3):653–661. doi: 10.1042/bj2380653. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Altin J. G., Bygrave F. L. The Ca2+-mobilizing actions of vasopressin and angiotensin differ from those of the alpha-adrenergic agonist phenylephrine in the perfused rat liver. Biochem J. 1985 Dec 15;232(3):911–917. doi: 10.1042/bj2320911. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Anwer M. S., Little J. M., Oelberg D. G., Zimniak P., Lester R. Effect of bile acids on calcium efflux from isolated rat hepatocytes and perfused rat livers. Proc Soc Exp Biol Med. 1989 Jun;191(2):147–152. doi: 10.3181/00379727-191-42900. [DOI] [PubMed] [Google Scholar]
  5. Combettes L., Berthon B., Doucet E., Erlinger S., Claret M. Bile acids mobilise internal Ca2+ independently of external Ca2+ in rat hepatocytes. Eur J Biochem. 1990 Jul 5;190(3):619–623. doi: 10.1111/j.1432-1033.1990.tb15617.x. [DOI] [PubMed] [Google Scholar]
  6. Combettes L., Berthon B., Doucet E., Erlinger S., Claret M. Characteristics of bile acid-mediated Ca2+ release from permeabilized liver cells and liver microsomes. J Biol Chem. 1989 Jan 5;264(1):157–167. [PubMed] [Google Scholar]
  7. Combettes L., Dumont M., Berthon B., Erlinger S., Claret M. Release of calcium from the endoplasmic reticulum by bile acids in rat liver cells. J Biol Chem. 1988 Feb 15;263(5):2299–2303. [PubMed] [Google Scholar]
  8. Drew R., Priestly B. G. Choleretic and cholestatic effects of infused bile salts in the rat. Experientia. 1979 Jun 15;35(6):809–811. doi: 10.1007/BF01968265. [DOI] [PubMed] [Google Scholar]
  9. Hamada Y., Karjalainen A., Setchell B. A., Millard J. E., Bygrave F. L. Concomitant stimulation by vasopressin of biliary and perfusate calcium fluxes in the perfused rat liver. Biochem J. 1992 Jan 15;281(Pt 2):387–392. doi: 10.1042/bj2810387. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hardison W. G., Dalle-Molle E., Gosink E., Lowe P. J., Steinbach J. H., Yamaguchi Y. Function of rat hepatocyte tight junctions: studies with bile acid infusions. Am J Physiol. 1991 Jan;260(1 Pt 1):G167–G174. doi: 10.1152/ajpgi.1991.260.1.G167. [DOI] [PubMed] [Google Scholar]
  11. Heuman D. M., Mills A. S., McCall J., Hylemon P. B., Pandak W. M., Vlahcevic Z. R. Conjugates of ursodeoxycholate protect against cholestasis and hepatocellular necrosis caused by more hydrophobic bile salts. In vivo studies in the rat. Gastroenterology. 1991 Jan;100(1):203–211. doi: 10.1016/0016-5085(91)90602-h. [DOI] [PubMed] [Google Scholar]
  12. Hill C. E., Dawson A. P., Pryor J. S. Evidence for adrenergic control of transcellular calcium distribution in liver. Biochem J. 1985 Sep 15;230(3):733–737. doi: 10.1042/bj2300733. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Kan K. S., Coleman R. The calcium ionophore A23187 increases the tight-junctional permeability in rat liver. Biochem J. 1988 Dec 15;256(3):1039–1041. doi: 10.1042/bj2561039. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Kitani K., Kanai S. Effect of ursodeoxycholate on the bile flow in the rat. Life Sci. 1982 Nov 1;31(18):1973–1985. doi: 10.1016/0024-3205(82)90036-4. [DOI] [PubMed] [Google Scholar]
  15. Llopis J., Kass G. E., Duddy S. K., Farell G. C., Gahm A., Orrenius S. Mobilization of the hormone-sensitive calcium pool increases hepatocyte tight junctional permeability in the perfused rat liver. FEBS Lett. 1991 Mar 11;280(1):84–86. doi: 10.1016/0014-5793(91)80209-l. [DOI] [PubMed] [Google Scholar]
  16. Lowe P. J., Miyai K., Steinbach J. H., Hardison W. G. Hormonal regulation of hepatocyte tight junctional permeability. Am J Physiol. 1988 Oct;255(4 Pt 1):G454–G461. doi: 10.1152/ajpgi.1988.255.4.G454. [DOI] [PubMed] [Google Scholar]
  17. Mauger J. P., Claret M. Calcium channels in hepatocytes. J Hepatol. 1988 Oct;7(2):278–282. doi: 10.1016/s0168-8278(88)80492-6. [DOI] [PubMed] [Google Scholar]
  18. Morgan N. G., Blackmore P. F., Exton J. H. Modulation of the alpha 1-adrenergic control of hepatocyte calcium redistribution by increases in cyclic AMP. J Biol Chem. 1983 Apr 25;258(8):5110–5116. [PubMed] [Google Scholar]
  19. Nathanson M. H., Boyer J. L. Mechanisms and regulation of bile secretion. Hepatology. 1991 Sep;14(3):551–566. [PubMed] [Google Scholar]
  20. Reinhart P. H., Taylor W. M., Bygrave F. L. Calcium ion fluxes induced by the action of alpha-adrenergic agonists in perfused rat liver. Biochem J. 1982 Dec 15;208(3):619–630. doi: 10.1042/bj2080619. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Reinhart P. H., Taylor W. M., Bygrave F. L. Studies on alpha-adrenergic-induced respiration and glycogenolysis in perfused rat liver. J Biol Chem. 1982 Feb 25;257(4):1906–1912. [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES