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. 1990 Mar 15;266(3):743–747. doi: 10.1042/bj2660743

The changes in hepatic enzyme expression caused by selenium deficiency and hypothyroidism in rats are produced by independent mechanisms.

G J Beckett 1, F Nicol 1, D Proudfoot 1, K Dyson 1, G Loucaides 1, J R Arthur 1
PMCID: PMC1131202  PMID: 2327962

Abstract

Selenium (Se) deficiency for 5 weeks in rats produced changes in the activity of a number of hepatic, renal and plasma enzymes. In animals whose food intake was restricted to 75% of normal for 2 weeks, Se deficiency produced significant increases in the activity of hepatic cytosolic 'malic' enzyme and mitochondrial alpha-glycerophosphate dehydrogenase (GPD), two enzymes that are particular sensitive to the thyroid-hormone concentrations in tissue. Propylthiouracil-induced hypothyroidism produced significant decreases in 'malic' enzyme and GPD activities. The effect of hypothyroidism on the activity of 'malic' enzyme, GPD and other enzymes studied in liver and plasma was often opposite to that seen in Se deficiency. Glutathione S-transferase (GST) activity was increased by both Se deficiency and hypothyroidism, but in hypothyroid animals further significant increases in GST were produced by Se deficiency. These data suggest that the changes in enzyme expression observed in Se deficiency are not caused by decreased tissue exposure to thyroid hormones.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arthur J. R., Boyne R. Superoxide dismutase and glutathione peroxidase activities in neutrophils from selenium deficient and copper deficient cattle. Life Sci. 1985 Apr 22;36(16):1569–1575. doi: 10.1016/0024-3205(85)90381-9. [DOI] [PubMed] [Google Scholar]
  2. Arthur J. R., Morrice P. C., Beckett G. J. Thyroid hormone concentrations in selenium deficient and selenium sufficient cattle. Res Vet Sci. 1988 Jul;45(1):122–123. [PubMed] [Google Scholar]
  3. Arthur J. R., Morrice P. C., Nicol F., Beddows S. E., Boyd R., Hayes J. D., Beckett G. J. The effects of selenium and copper deficiencies on glutathione S-transferase and glutathione peroxidase in rat liver. Biochem J. 1987 Dec 1;248(2):539–544. doi: 10.1042/bj2480539. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Beckett G. J., Beddows S. E., Morrice P. C., Nicol F., Arthur J. R. Inhibition of hepatic deiodination of thyroxine is caused by selenium deficiency in rats. Biochem J. 1987 Dec 1;248(2):443–447. doi: 10.1042/bj2480443. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Beckett G. J., MacDougall D. A., Nicol F., Arthur R. Inhibition of type I and type II iodothyronine deiodinase activity in rat liver, kidney and brain produced by selenium deficiency. Biochem J. 1989 May 1;259(3):887–892. doi: 10.1042/bj2590887. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Doran G. R., Wilkinson J. H. The origin of the elevated activities of creatine kinase and other enzymes in the sera of patients with myxoedema. Clin Chim Acta. 1975 Jul 23;62(2):203–211. doi: 10.1016/0009-8981(75)90229-6. [DOI] [PubMed] [Google Scholar]
  7. Habig W. H., Pabst M. J., Jakoby W. B. Glutathione S-transferases. The first enzymatic step in mercapturic acid formation. J Biol Chem. 1974 Nov 25;249(22):7130–7139. [PubMed] [Google Scholar]
  8. Hafeman D. G., Hoekstra W. G. Protection against carbon tetrachloride-induced lipid peroxidation in the rat by dietary vitamin E, selenium, and methionine as measured by ethane evolution. J Nutr. 1977 Apr;107(4):656–665. doi: 10.1093/jn/107.4.656. [DOI] [PubMed] [Google Scholar]
  9. Hill K. E., Burk R. F., Lane J. M. Effect of selenium depletion and repletion on plasma glutathione and glutathione-dependent enzymes in the rat. J Nutr. 1987 Jan;117(1):99–104. doi: 10.1093/jn/117.1.99. [DOI] [PubMed] [Google Scholar]
  10. LEE Y. P., LARDY H. A. INFLUENCE OF THYROID HORMONES ON L-ALPHA-GLYCEROPHOSPHATE DEHYDROGENASES AND OTHER DEHYDROGENASES IN VARIOUS ORGANS OF THE RAT. J Biol Chem. 1965 Mar;240:1427–1436. [PubMed] [Google Scholar]
  11. Lawrence R. A., Parkhill L. K., Burk R. F. Hepatic cytosolic non selenium-dependent glutathione peroxidase activity: its nature and the effect of selenium deficiency. J Nutr. 1978 Jun;108(6):981–987. doi: 10.1093/jn/108.6.981. [DOI] [PubMed] [Google Scholar]
  12. Masukawa T., Nishimura T., Iwata H. Differential changes of glutathione S-transferase activity by dietary selenium. Biochem Pharmacol. 1984 Aug 15;33(16):2635–2639. doi: 10.1016/0006-2952(84)90637-3. [DOI] [PubMed] [Google Scholar]
  13. Ratcliffe W. A., Challand G. S., Ratcliffe J. G. A critical evaluation of separation methods in radiommunoassay for total triiodothyronine and thyroxine in unextracted human serum. Ann Clin Biochem. 1974 Nov;11(6):224–229. doi: 10.1177/000456327401100166. [DOI] [PubMed] [Google Scholar]
  14. Reiter R., Wendel A. Selenium and drug metabolism--I. Multiple modulations of mouse liver enzymes. Biochem Pharmacol. 1983 Oct 15;32(20):3063–3067. doi: 10.1016/0006-2952(83)90250-2. [DOI] [PubMed] [Google Scholar]
  15. Reiter R., Wendel A. Selenium and drug metabolism--II. Independence of glutathione peroxidase and reversibility of hepatic enzyme modulations in deficient mice. Biochem Pharmacol. 1984 Jun 15;33(12):1923–1928. doi: 10.1016/0006-2952(84)90548-3. [DOI] [PubMed] [Google Scholar]
  16. Reiter R., Wendel A. Selenium and drug metabolism--III. Relation of glutathione-peroxidase and other hepatic enzyme modulations to dietary supplements. Biochem Pharmacol. 1985 Jul 1;34(13):2287–2290. doi: 10.1016/0006-2952(85)90783-x. [DOI] [PubMed] [Google Scholar]
  17. Tarentino A. L., Richert D. A., Westerfeld W. W. The concurrent induction of hepatic alpha-glycerophosphate dehydrogenase and malate dehydrogenase by thyroid hormone. Biochim Biophys Acta. 1966 Aug 24;124(2):205–309. [PubMed] [Google Scholar]
  18. Williams M. T., Carrington H., Herrera A. Stimulation of mouse liver glutathione S-transferase activity in propylthiouracil-treated mice in vivo by tri-iodothyronine. Biochem J. 1986 Jan 15;233(2):595–598. doi: 10.1042/bj2330595. [DOI] [PMC free article] [PubMed] [Google Scholar]

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