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. 1990 Apr 1;267(1):269–271. doi: 10.1042/bj2670269

Oral administration of vanadate to streptozotocin-diabetic rats restores the glucose-induced activation of liver glycogen synthase.

M Bollen 1, M Miralpeix 1, F Ventura 1, B Toth 1, R Bartrons 1, W Stalmans 1
PMCID: PMC1131277  PMID: 2109604

Abstract

Isolated hepatocytes from streptozotocin-diabetic rats failed to respond to a glucose load with an activation of glycogen synthase. This lesion was associated with severely decreased activities of glycogen-synthase phosphatase and of glucokinase. All these defects were abolished after consumption for 13-18 days of drinking water containing Na3VO4 (0.7 mg/ml), and they were partially restored after 3.5 days, when the blood glucose concentration was already normalized. In all conditions the maximal extent of activation of glycogen synthase in cells closely parallelled the activity of glycogen-synthase phosphatase.

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Selected References

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  1. Bendayan M., Gingras D. Effect of vanadate administration on blood glucose and insulin levels as well as on the exocrine pancreatic function in streptozotocin-diabetic rats. Diabetologia. 1989 Aug;32(8):561–567. doi: 10.1007/BF00285328. [DOI] [PubMed] [Google Scholar]
  2. Bollen M., Hue L., Stalmans W. Effects of glucose on phosphorylase and glycogen synthase in hepatocytes from diabetic rats. Biochem J. 1983 Mar 15;210(3):783–787. doi: 10.1042/bj2100783. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bollen M., Keppens S., Stalmans W. Differences in liver glycogen-synthase phosphatase activity in rodents with spontaneous insulin-dependent and non-insulin-dependent diabetes. Diabetologia. 1988 Sep;31(9):711–713. doi: 10.1007/BF00278757. [DOI] [PubMed] [Google Scholar]
  4. Bollen M., Stalmans W. The hepatic defect in glycogen synthesis in chronic diabetes involves the G-component of synthase phosphatase. Biochem J. 1984 Jan 15;217(2):427–434. doi: 10.1042/bj2170427. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Bosch F., Ariño J., Gómez-Foix A. M., Guinovart J. J. Glycogenolytic, noninsulin-like effects of vanadate on rat hepatocyte glycogen synthase and phosphorylase. J Biol Chem. 1987 Jan 5;262(1):218–222. [PubMed] [Google Scholar]
  6. Brichard S. M., Okitolonda W., Henquin J. C. Long term improvement of glucose homeostasis by vanadate treatment in diabetic rats. Endocrinology. 1988 Oct;123(4):2048–2053. doi: 10.1210/endo-123-4-2048. [DOI] [PubMed] [Google Scholar]
  7. Clark A. S., Fagan J. M., Mitch W. E. Selectivity of the insulin-like actions of vanadate on glucose and protein metabolism in skeletal muscle. Biochem J. 1985 Nov 15;232(1):273–276. doi: 10.1042/bj2320273. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Doperé F., Vanstapel F., Stalmans W. Glycogen-synthase phosphatase activity in rat liver. Two protein components and their requirement for the activation of different types of substrate. Eur J Biochem. 1980 Feb;104(1):137–146. doi: 10.1111/j.1432-1033.1980.tb04409.x. [DOI] [PubMed] [Google Scholar]
  9. Gil J., Miralpeix M., Carreras J., Bartrons R. Insulin-like effects of vanadate on glucokinase activity and fructose 2,6-bisphosphate levels in the liver of diabetic rats. J Biol Chem. 1988 Feb 5;263(4):1868–1871. [PubMed] [Google Scholar]
  10. Green A. The insulin-like effect of sodium vanadate on adipocyte glucose transport is mediated at a post-insulin-receptor level. Biochem J. 1986 Sep 15;238(3):663–669. doi: 10.1042/bj2380663. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Heyliger C. E., Tahiliani A. G., McNeill J. H. Effect of vanadate on elevated blood glucose and depressed cardiac performance of diabetic rats. Science. 1985 Mar 22;227(4693):1474–1477. doi: 10.1126/science.3156405. [DOI] [PubMed] [Google Scholar]
  12. Jackson T. K., Salhanick A. I., Sparks J. D., Sparks C. E., Bolognino M., Amatruda J. M. Insulin-mimetic effects of vanadate in primary cultures of rat hepatocytes. Diabetes. 1988 Sep;37(9):1234–1240. doi: 10.2337/diab.37.9.1234. [DOI] [PubMed] [Google Scholar]
  13. Meyerovitch J., Farfel Z., Sack J., Shechter Y. Oral administration of vanadate normalizes blood glucose levels in streptozotocin-treated rats. Characterization and mode of action. J Biol Chem. 1987 May 15;262(14):6658–6662. [PubMed] [Google Scholar]
  14. Miller T. B., Jr Effects of diabetes on glucose regulation of enzymes involved in hepatic glycogen metabolism. Am J Physiol. 1978 Jan;234(1):E13–E19. doi: 10.1152/ajpendo.1978.234.1.E13. [DOI] [PubMed] [Google Scholar]
  15. Miralpeix M., Gil J., Rosa J. L., Carreras J., Bartrons R. Vanadate counteracts glucagon effects in isolated rat hepatocytes. Life Sci. 1989;44(20):1491–1497. doi: 10.1016/0024-3205(89)90328-7. [DOI] [PubMed] [Google Scholar]
  16. Mooney R. A., Bordwell K. L., Luhowskyj S., Casnellie J. E. The insulin-like effect of vanadate on lipolysis in rat adipocytes is not accompanied by an insulin-like effect on tyrosine phosphorylation. Endocrinology. 1989 Jan;124(1):422–429. doi: 10.1210/endo-124-1-422. [DOI] [PubMed] [Google Scholar]
  17. Role of vanadium in biology. Symposium summary. Fed Proc. 1986 Feb;45(2):123–132. [PubMed] [Google Scholar]
  18. Rossetti L., Lauglin M. R. Correction of chronic hyperglycemia with vanadate, but not with phlorizin, normalizes in vivo glycogen repletion and in vitro glycogen synthase activity in diabetic skeletal muscle. J Clin Invest. 1989 Sep;84(3):892–899. doi: 10.1172/JCI114250. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Strout H. V., Vicario P. P., Saperstein R., Slater E. E. The insulin-mimetic effect of vanadate is not correlated with insulin receptor tyrosine kinase activity nor phosphorylation in mouse diaphragm in vivo. Endocrinology. 1989 Apr;124(4):1918–1924. doi: 10.1210/endo-124-4-1918. [DOI] [PubMed] [Google Scholar]
  20. Tamura S., Brown T. A., Whipple J. H., Fujita-Yamaguchi Y., Dubler R. E., Cheng K., Larner J. A novel mechanism for the insulin-like effect of vanadate on glycogen synthase in rat adipocytes. J Biol Chem. 1984 May 25;259(10):6650–6658. [PubMed] [Google Scholar]
  21. Tolman E. L., Barris E., Burns M., Pansini A., Partridge R. Effects of vanadium on glucose metabolism in vitro. Life Sci. 1979 Sep 24;25(13):1159–1164. doi: 10.1016/0024-3205(79)90138-3. [DOI] [PubMed] [Google Scholar]
  22. Tóth B., Bollen M., Stalmans W. Acute regulation of hepatic protein phosphatases by glucagon, insulin, and glucose. J Biol Chem. 1988 Oct 5;263(28):14061–14066. [PubMed] [Google Scholar]

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