Skip to main content
PMC home page
Biochemical Journal logoLink to Biochemical Journal
. 1990 Apr 15;267(2):317–323. doi: 10.1042/bj2670317

Transcriptional regulation of rat liver protein disulphide-isomerase gene by insulin and in diabetes.

A Nieto 1, E Mira 1, J G Castaño 1
PMCID: PMC1131289  PMID: 1692206

Abstract

The mRNA encoding for rat protein disulphide-isomerase (PDI) increases 3-fold in the liver of diabetic rats and is accompanied by similar changes at the protein level. Long treatment (for 3 days) of diabetic rats with insulin reverses this effect of diabetes both at the mRNA and protein levels. The higher expression of rat PDI mRNA in diabetes is due to an increase in the transcriptional rate of the gene, and insulin treatment of diabetic animals produces within 30 min a decrease in the level of transcription of PDI gene, as judged by nuclear run-on transcription experiments performed in vivo. These results clearly show a role for insulin in the regulation of transcription of the gene encoding this multifunctional protein in rat liver.

Full text

PDF
322

Images in this article

319Fig. 1.
on p.319
320Fig. 2.
on p.320
320Fig. 3.
on p.320
321Fig. 4.
on p.321
321Fig. 5.
on p.321

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adams S. L., Sobel M. E., Howard B. H., Olden K., Yamada K. M., de Crombrugghe B., Pastan I. Levels of translatable mRNAs for cell surface protein, collagen precursors, and two membrane proteins are altered in Rous sarcoma virus-transformed chick embryo fibroblasts. Proc Natl Acad Sci U S A. 1977 Aug;74(8):3399–3403. doi: 10.1073/pnas.74.8.3399. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Anfinsen C. B., Scheraga H. A. Experimental and theoretical aspects of protein folding. Adv Protein Chem. 1975;29:205–300. doi: 10.1016/s0065-3233(08)60413-1. [DOI] [PubMed] [Google Scholar]
  3. Boado R. J., Campbell D. A., Chopra I. J. Nucleotide sequence of rat liver iodothyronine 5'-monodeiodinase (5' MD): its identity with the protein disulfide isomerase. Biochem Biophys Res Commun. 1988 Sep 30;155(3):1297–1304. doi: 10.1016/s0006-291x(88)81282-8. [DOI] [PubMed] [Google Scholar]
  4. Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. doi: 10.1006/abio.1976.9999. [DOI] [PubMed] [Google Scholar]
  5. Brownlee M., Cerami A. The biochemistry of the complications of diabetes mellitus. Annu Rev Biochem. 1981;50:385–432. doi: 10.1146/annurev.bi.50.070181.002125. [DOI] [PubMed] [Google Scholar]
  6. Bulleid N. J., Freedman R. B. Defective co-translational formation of disulphide bonds in protein disulphide-isomerase-deficient microsomes. Nature. 1988 Oct 13;335(6191):649–651. doi: 10.1038/335649a0. [DOI] [PubMed] [Google Scholar]
  7. Cheng S. Y., Gong Q. H., Parkison C., Robinson E. A., Appella E., Merlino G. T., Pastan I. The nucleotide sequence of a human cellular thyroid hormone binding protein present in endoplasmic reticulum. J Biol Chem. 1987 Aug 15;262(23):11221–11227. [PubMed] [Google Scholar]
  8. Cimbala M. A., Lamers W. H., Nelson K., Monahan J. E., Yoo-Warren H., Hanson R. W. Rapid changes in the concentration of phosphoenolpyruvate carboxykinase mRNA in rat liver and kidney. Effects of insulin and cyclic AMP. J Biol Chem. 1982 Jul 10;257(13):7629–7636. [PubMed] [Google Scholar]
  9. Edman J. C., Ellis L., Blacher R. W., Roth R. A., Rutter W. J. Sequence of protein disulphide isomerase and implications of its relationship to thioredoxin. Nature. 1985 Sep 19;317(6034):267–270. doi: 10.1038/317267a0. [DOI] [PubMed] [Google Scholar]
  10. Feinberg A. P., Vogelstein B. "A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity". Addendum. Anal Biochem. 1984 Feb;137(1):266–267. doi: 10.1016/0003-2697(84)90381-6. [DOI] [PubMed] [Google Scholar]
  11. Freedman R. B., Brockway B. E., Lambert N. Protein disulphide-isomerase and the formation of native disulphide bonds. Biochem Soc Trans. 1984 Dec;12(6):929–932. doi: 10.1042/bst0120929. [DOI] [PubMed] [Google Scholar]
  12. Freedman R. B. Protein disulfide isomerase: multiple roles in the modification of nascent secretory proteins. Cell. 1989 Jun 30;57(7):1069–1072. doi: 10.1016/0092-8674(89)90043-3. [DOI] [PubMed] [Google Scholar]
  13. Geetha-Habib M., Noiva R., Kaplan H. A., Lennarz W. J. Glycosylation site binding protein, a component of oligosaccharyl transferase, is highly similar to three other 57 kd luminal proteins of the ER. Cell. 1988 Sep 23;54(7):1053–1060. doi: 10.1016/0092-8674(88)90120-1. [DOI] [PubMed] [Google Scholar]
  14. Gibson R., Leavitt R., Kornfeld S., Schlesinger S. Synthesis and infectivity of vesicular stomatitis virus containing nonglycosylated G protein. Cell. 1978 Apr;13(4):671–679. doi: 10.1016/0092-8674(78)90217-9. [DOI] [PubMed] [Google Scholar]
  15. Granner D., Andreone T., Sasaki K., Beale E. Inhibition of transcription of the phosphoenolpyruvate carboxykinase gene by insulin. Nature. 1983 Oct 6;305(5934):549–551. doi: 10.1038/305549a0. [DOI] [PubMed] [Google Scholar]
  16. Günzler V., Brocks D., Henke S., Myllylä R., Geiger R., Kivirikko K. I. Syncatalytic inactivation of prolyl 4-hydroxylase by synthetic peptides containing the unphysiologic amino acid 5-oxaproline. J Biol Chem. 1988 Dec 25;263(36):19498–19504. [PubMed] [Google Scholar]
  17. Hickman S., Kulczycki A., Jr, Lynch R. G., Kornfeld S. Studies of the mechanism of tunicamycin in hibition of IgA and IgE secretion by plasma cells. J Biol Chem. 1977 Jun 25;252(12):4402–4408. [PubMed] [Google Scholar]
  18. Holroyde M. J., Allen M. B., Storer A. C., Warsy A. S., Chesher J. M., Trayer I. P., Cornish-Bowden A., Walker D. G. The purification in high yield and characterization of rat hepatic glucokinase. Biochem J. 1976 Feb 1;153(2):363–373. doi: 10.1042/bj1530363. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Iynedjian P. B., Möbius G., Seitz H. J., Wollheim C. B., Renold A. E. Tissue-specific expression of glucokinase: identification of the gene product in liver and pancreatic islets. Proc Natl Acad Sci U S A. 1986 Apr;83(7):1998–2001. doi: 10.1073/pnas.83.7.1998. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Jefferson L. S., Liao W. S., Peavy D. E., Miller T. B., Appel M. C., Taylor J. M. Diabetes-induced alterations in liver protein synthesis. Changes in the relative abundance of mRNAs for albumin and other plasma proteins. J Biol Chem. 1983 Jan 25;258(2):1369–1375. [PubMed] [Google Scholar]
  21. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  22. Lamers W. H., Hanson R. W., Meisner H. M. cAMP stimulates transcription of the gene for cytosolic phosphoenolpyruvate carboxykinase in rat liver nuclei. Proc Natl Acad Sci U S A. 1982 Sep;79(17):5137–5141. doi: 10.1073/pnas.79.17.5137. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Maxam A. M., Gilbert W. Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol. 1980;65(1):499–560. doi: 10.1016/s0076-6879(80)65059-9. [DOI] [PubMed] [Google Scholar]
  24. Mira E., Castaño J. G. Insulin short-term control of rat liver alpha 2-microglobulin gene transcription. J Biol Chem. 1989 Oct 25;264(30):18209–18212. [PubMed] [Google Scholar]
  25. O'Farrell P. Z., Goodman H. M., O'Farrell P. H. High resolution two-dimensional electrophoresis of basic as well as acidic proteins. Cell. 1977 Dec;12(4):1133–1141. doi: 10.1016/0092-8674(77)90176-3. [DOI] [PubMed] [Google Scholar]
  26. Obata T., Kitagawa S., Gong Q. H., Pastan I., Cheng S. Y. Thyroid hormone down-regulates p55, a thyroid hormone-binding protein that is homologous to protein disulfide isomerase and the beta-subunit of prolyl-4-hydroxylase. J Biol Chem. 1988 Jan 15;263(2):782–785. [PubMed] [Google Scholar]
  27. Ohba H., Harano T., Omura T. Biosynthesis and turnover of a microsomal protein disulfide isomerase in rat liver. J Biochem. 1981 Mar;89(3):901–907. doi: 10.1093/oxfordjournals.jbchem.a133273. [DOI] [PubMed] [Google Scholar]
  28. Peavy D. E., Taylor J. M., Jefferson L. S. Correlation of albumin production rates and albumin mRNA levels in livers of normal, diabetic, and insulin-treated diabetic rats. Proc Natl Acad Sci U S A. 1978 Dec;75(12):5879–5883. doi: 10.1073/pnas.75.12.5879. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Pihlajaniemi T., Helaakoski T., Tasanen K., Myllylä R., Huhtala M. L., Koivu J., Kivirikko K. I. Molecular cloning of the beta-subunit of human prolyl 4-hydroxylase. This subunit and protein disulphide isomerase are products of the same gene. EMBO J. 1987 Mar;6(3):643–649. doi: 10.1002/j.1460-2075.1987.tb04803.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Prager D., Melmed S. Insulin regulates expression of the human growth hormone gene in transfected cells. J Biol Chem. 1988 Nov 15;263(32):16580–16585. [PubMed] [Google Scholar]
  31. Roy A. K., Chatterjee B., Prasad M. S., Unakar N. J. Role of insulin in the regulation of the hepatic messenger RNA for alpha 2u-globulin in diabetic rats. J Biol Chem. 1980 Dec 10;255(23):11614–11618. [PubMed] [Google Scholar]
  32. Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Sharma C., Dalferes E. R., Jr, Radhakrishnamurthy B., DePaolo C. J., Berenson G. S. Hepatic glycoprotein synthesis in streptozotocin diabetic rats. Biochem Int. 1987 Aug;15(2):395–401. [PubMed] [Google Scholar]
  34. Towbin H., Staehelin T., Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350–4354. doi: 10.1073/pnas.76.9.4350. [DOI] [PMC free article] [PubMed] [Google Scholar]
  35. Unterman R. D., Lynch K. R., Nakhasi H. L., Dolan K. P., Hamilton J. W., Cohn D. V., Feigelson P. Cloning and sequence of several alpha 2u-globulin cDNAs. Proc Natl Acad Sci U S A. 1981 Jun;78(6):3478–3482. doi: 10.1073/pnas.78.6.3478. [DOI] [PMC free article] [PubMed] [Google Scholar]
  36. Weinhouse S. Regulation of glucokinase in liver. Curr Top Cell Regul. 1976;11:1–50. [PubMed] [Google Scholar]
  37. Wilbur W. J., Lipman D. J. Rapid similarity searches of nucleic acid and protein data banks. Proc Natl Acad Sci U S A. 1983 Feb;80(3):726–730. doi: 10.1073/pnas.80.3.726. [DOI] [PMC free article] [PubMed] [Google Scholar]
  38. Yamauchi K., Yamamoto T., Hayashi H., Koya S., Takikawa H., Toyoshima K., Horiuchi R. Sequence of membrane-associated thyroid hormone binding protein from bovine liver: its identity with protein disulphide isomerase. Biochem Biophys Res Commun. 1987 Aug 14;146(3):1485–1492. doi: 10.1016/0006-291x(87)90817-5. [DOI] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES