Skip to main content
PMC home page
Biochemical Journal logoLink to Biochemical Journal
. 1990 Aug 1;269(3):561–564. doi: 10.1042/bj2690561

Hydrodynamic properties of connective-tissue polysaccharides.

W D Comper 1, O Zamparo 1
PMCID: PMC1131623  PMID: 2117916

Abstract

The major hydrodynamic properties of the connective-tissue polysaccharides are those that describe polysaccharide-water interaction as embodied in their osmotic-pressure and hydraulic-conductivity properties. This study shows that, for polysaccharides such as chondroitin sulphate, hyaluronate and the heparin-like polysaccharides, their hydrodynamic properties depend primarily on the presence of the uronic residue and the nature of the glycosidic linkage. Other parameters such as the degree of N-acetylation and sulphation were found not to influence these properties to any great extent. These studies particularly delineate structural-functional aspects of the connective-tissue polysaccharides in terms of their primary structure.

Full text

PDF

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Avila J. L., Convit J. Inhibition of leucocytic lysosomal enzymes by glycosaminoglycans in vitro. Biochem J. 1975 Oct;152(1):57–64. doi: 10.1042/bj1520057. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Avila J. L., Convit J. Physicochemical characteristics of the glycosaminoglycan-lysosomal enzyme interaction in vitro. A model of control of leucocytic lysosomal activity. Biochem J. 1976 Nov 15;160(2):129–136. doi: 10.1042/bj1600129. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Blackwell J., Schodt K. P., Gelman R. A. Polysaccharide-polypeptide systems as models for heparin interactions. Fed Proc. 1977 Jan;36(1):98–101. [PubMed] [Google Scholar]
  4. Comper W. D., Laurent T. C. Physiological function of connective tissue polysaccharides. Physiol Rev. 1978 Jan;58(1):255–315. doi: 10.1152/physrev.1978.58.1.255. [DOI] [PubMed] [Google Scholar]
  5. Comper W. D., Williams R. P. Hydrodynamics of concentrated proteoglycan solutions. J Biol Chem. 1987 Oct 5;262(28):13464–13471. [PubMed] [Google Scholar]
  6. Elbein A. D. Interactions of polynucleotides and other polyelectrolytes with enzymes and other proteins. Adv Enzymol Relat Areas Mol Biol. 1974;40(0):29–64. doi: 10.1002/9780470122853.ch2. [DOI] [PubMed] [Google Scholar]
  7. Farndale R. W., Sayers C. A., Barrett A. J. A direct spectrophotometric microassay for sulfated glycosaminoglycans in cartilage cultures. Connect Tissue Res. 1982;9(4):247–248. doi: 10.3109/03008208209160269. [DOI] [PubMed] [Google Scholar]
  8. Lindahl U., Hök M. Glycosaminoglycans and their binding to biological macromolecules. Annu Rev Biochem. 1978;47:385–417. doi: 10.1146/annurev.bi.47.070178.002125. [DOI] [PubMed] [Google Scholar]
  9. Ruoslahti E. Structure and biology of proteoglycans. Annu Rev Cell Biol. 1988;4:229–255. doi: 10.1146/annurev.cb.04.110188.001305. [DOI] [PubMed] [Google Scholar]
  10. Taylor R. L., Shively J. E., Conrad H. E., Cifonelli J. A. Uronic acid composition of heparins and heparan sulfates. Biochemistry. 1973 Sep 11;12(19):3633–3637. doi: 10.1021/bi00743a010. [DOI] [PubMed] [Google Scholar]
  11. Urban J. P., Maroudas A., Bayliss M. T., Dillon J. Swelling pressures of proteoglycans at the concentrations found in cartilaginous tissues. Biorheology. 1979;16(6):447–464. doi: 10.3233/bir-1979-16609. [DOI] [PubMed] [Google Scholar]
  12. Zamparo O., Comper W. D. Hydraulic conductivity of chondroitin sulfate proteoglycan solutions. Arch Biochem Biophys. 1989 Oct;274(1):259–269. doi: 10.1016/0003-9861(89)90438-4. [DOI] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES