Skip to main content
PMC home page
Biochemical Journal logoLink to Biochemical Journal
. 1990 Aug 1;269(3):831–834. doi: 10.1042/bj2690831

The novel inositol lipid phosphatidylinositol 3,4-bisphosphate is produced by human blood platelets upon thrombin stimulation.

C Sultan 1, M Breton 1, G Mauco 1, P Grondin 1, M Plantavid 1, H Chap 1
PMCID: PMC1131663  PMID: 2167665

Abstract

Radioactive PtdIns(3)P was detected in human platelets incubated with [32P]Pi, but remained unaffected by thrombin treatment. In contrast, [32P]PtdIns(3,4)P2 was absent from resting platelets, but was produced by thrombin-activated platelets in a dose- and time-dependent manner. [32P]PtdInsP3 was never found under these conditions. These changes are similar to those elicited in other cells by platelet-derived growth factor or the oncogene product pp60c-src.

Full text

PDF
832

Images in this article

832Fig. 2.
on p.832

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ASTER R. H., JANDL J. H. PLATELET SEQUESTRATION IN MAN. I. METHODS. J Clin Invest. 1964 May;43:843–855. doi: 10.1172/JCI104970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Ardlie N. G., Packham M. A., Mustard J. F. Adenosine diphosphate-induced platelet aggregation in suspensions of washed rabbit platelets. Br J Haematol. 1970 Jul;19(1):7–17. doi: 10.1111/j.1365-2141.1970.tb01596.x. [DOI] [PubMed] [Google Scholar]
  3. Auger K. R., Serunian L. A., Soltoff S. P., Libby P., Cantley L. C. PDGF-dependent tyrosine phosphorylation stimulates production of novel polyphosphoinositides in intact cells. Cell. 1989 Apr 7;57(1):167–175. doi: 10.1016/0092-8674(89)90182-7. [DOI] [PubMed] [Google Scholar]
  4. Berridge M. J. The Albert Lasker Medical Awards. Inositol trisphosphate, calcium, lithium, and cell signaling. JAMA. 1989 Oct 6;262(13):1834–1841. [PubMed] [Google Scholar]
  5. Bryckaert M. C., Rendu F., Tobelem G., Caen J. PDGF modifies phosphoinositide metabolism and inhibits aggregation and release in human platelets. Biochem Biophys Res Commun. 1986 Feb 26;135(1):52–57. doi: 10.1016/0006-291x(86)90941-1. [DOI] [PubMed] [Google Scholar]
  6. Bryckaert M. C., Rendu F., Tobelem G., Wasteson A. Collagen-induced binding to human platelets of platelet-derived growth factor leading to inhibition of P43 and P20 phosphorylation. J Biol Chem. 1989 Mar 15;264(8):4336–4341. [PubMed] [Google Scholar]
  7. Coughlin S. R., Escobedo J. A., Williams L. T. Role of phosphatidylinositol kinase in PDGF receptor signal transduction. Science. 1989 Mar 3;243(4895):1191–1194. doi: 10.1126/science.2466336. [DOI] [PubMed] [Google Scholar]
  8. Courtneidge S. A., Heber A. An 81 kd protein complexed with middle T antigen and pp60c-src: a possible phosphatidylinositol kinase. Cell. 1987 Sep 25;50(7):1031–1037. doi: 10.1016/0092-8674(87)90169-3. [DOI] [PubMed] [Google Scholar]
  9. Golden A., Nemeth S. P., Brugge J. S. Blood platelets express high levels of the pp60c-src-specific tyrosine kinase activity. Proc Natl Acad Sci U S A. 1986 Feb;83(4):852–856. doi: 10.1073/pnas.83.4.852. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Gonzalez-Sastre F., Folch-Pi J. Thin-layer chromatography of the phosphoinositides. J Lipid Res. 1968 Jul;9(4):532–533. [PubMed] [Google Scholar]
  11. HOKIN L. E., HOKIN M. R. Effects of acetylcholine on the turnover of phosphoryl units in individual phospholipids of pancreas slices and brain cortex slices. Biochim Biophys Acta. 1955 Sep;18(1):102–110. doi: 10.1016/0006-3002(55)90013-5. [DOI] [PubMed] [Google Scholar]
  12. Kaplan D. R., Whitman M., Schaffhausen B., Pallas D. C., White M., Cantley L., Roberts T. M. Common elements in growth factor stimulation and oncogenic transformation: 85 kd phosphoprotein and phosphatidylinositol kinase activity. Cell. 1987 Sep 25;50(7):1021–1029. doi: 10.1016/0092-8674(87)90168-1. [DOI] [PubMed] [Google Scholar]
  13. Mauco G., Chap H., Simon M. F., Douste-Blazy L. Phosphatidic and lysophosphatidic acid production in phospholipase C-and thrombin-treated platelets. Possible involvement of a platelet lipase. Biochimie. 1978 Sep 29;60(6-7):653–661. doi: 10.1016/s0300-9084(78)80784-6. [DOI] [PubMed] [Google Scholar]
  14. Mauco G., Dajeans P., Chap H., Douste-Blazy L. Subcellular localization of inositol lipids in blood platelets as deduced from the use of labelled precursors. Biochem J. 1987 Jun 15;244(3):757–761. doi: 10.1042/bj2440757. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Michell R. H. Inositol phospholipids and cell surface receptor function. Biochim Biophys Acta. 1975 Mar 25;415(1):81–47. doi: 10.1016/0304-4157(75)90017-9. [DOI] [PubMed] [Google Scholar]
  16. Nolan R. D., Lapetina E. G. Thrombin stimulates the production of a novel polyphosphoinositide in human platelets. J Biol Chem. 1990 Feb 15;265(5):2441–2445. [PubMed] [Google Scholar]
  17. Parsons S. J., Creutz C. E. p60c-src activity detected in the chromaffin granule membrane. Biochem Biophys Res Commun. 1986 Jan 29;134(2):736–742. doi: 10.1016/s0006-291x(86)80482-x. [DOI] [PubMed] [Google Scholar]
  18. Pignataro O. P., Ascoli M. Epidermal growth factor increases the labeling of phosphatidylinositol 3,4-bisphosphate in MA-10 Leydig tumor cells. J Biol Chem. 1990 Jan 25;265(3):1718–1723. [PubMed] [Google Scholar]
  19. Rana R. S., Hokin L. E. Role of phosphoinositides in transmembrane signaling. Physiol Rev. 1990 Jan;70(1):115–164. doi: 10.1152/physrev.1990.70.1.115. [DOI] [PubMed] [Google Scholar]
  20. Rendu F., Lebret M., Danielian S., Fagard R., Levy-Toledano S., Fischer S. High pp60c-src level in human platelet dense bodies. Blood. 1989 May 1;73(6):1545–1551. [PubMed] [Google Scholar]
  21. Schacht J. Extraction and purification of polyphosphoinositides. Methods Enzymol. 1981;72:626–631. doi: 10.1016/s0076-6879(81)72054-8. [DOI] [PubMed] [Google Scholar]
  22. Tysnes O. B., Verhoeven A. J., Aarbakke G. M., Holmsen H. Phosphoinositide metabolism in resting and thrombin-stimulated human platelets. Evidence against metabolic heterogeneity. FEBS Lett. 1987 Jun 22;218(1):68–72. doi: 10.1016/0014-5793(87)81020-7. [DOI] [PubMed] [Google Scholar]
  23. Verhoeven A. J., Tysnes O. B., Aarbakke G. M., Cook C. A., Holmsen H. Turnover of the phosphomonoester groups of polyphosphoinositol lipids in unstimulated human platelets. Eur J Biochem. 1987 Jul 1;166(1):3–9. doi: 10.1111/j.1432-1033.1987.tb13475.x. [DOI] [PubMed] [Google Scholar]
  24. Whitman M., Downes C. P., Keeler M., Keller T., Cantley L. Type I phosphatidylinositol kinase makes a novel inositol phospholipid, phosphatidylinositol-3-phosphate. Nature. 1988 Apr 14;332(6165):644–646. doi: 10.1038/332644a0. [DOI] [PubMed] [Google Scholar]
  25. Whitman M., Kaplan D., Roberts T., Cantley L. Evidence for two distinct phosphatidylinositol kinases in fibroblasts. Implications for cellular regulation. Biochem J. 1987 Oct 1;247(1):165–174. doi: 10.1042/bj2470165. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES