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. 1990 Aug 15;270(1):97–102. doi: 10.1042/bj2700097

Identification, sequencing and expression of an integral membrane protein of the trans-Golgi network (TGN38).

J P Luzio 1, B Brake 1, G Banting 1, K E Howell 1, P Braghetta 1, K K Stanley 1
PMCID: PMC1131683  PMID: 2204342

Abstract

Organelle-specific integral membrane proteins were identified by a novel strategy which gives rise to monospecific antibodies to these proteins as well as to the cDNA clones encoding them. A cDNA expression library was screened with a polyclonal antiserum raised against Triton X-114-extracted organelle proteins and clones were then grouped using antibodies affinity-purified on individual fusion proteins. The identification, molecular cloning and sequencing are described of a type 1 membrane protein (TGN38) which is located specifically in the trans-Golgi network.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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