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. 1992 Nov 15;288(Pt 1):19–22. doi: 10.1042/bj2880019

Trehalase from male accessory gland of an insect, Tenebrio molitor. cDNA sequencing and developmental profile of the gene expression.

M Takiguchi 1, T Niimi 1, Z H Su 1, T Yaginuma 1
PMCID: PMC1132073  PMID: 1445264

Abstract

A cDNA of alpha alpha-trehalase (EC 3.2.1.28) from a cDNA library of male bean-shaped accessory gland of the mealworm beetle, Tenebrio molitor, has been isolated by the homology screening approach. Sequence analysis of the cDNA (1830 bp) revealed that the cDNA encoded a protein of 555 amino acids with a calculated M(r) of 64457. The deduced amino acid sequence had significant similarities to rabbit small intestine and Escherichia coli trehalases. Northern blotting and semi-quantitative PCR analyses revealed that a trehalase transcript with about 2.0 kb was abundant in bean-shaped accessory glands. In the glands, the amount of trehalase transcript increased from 1 to 2 days after adult ecdysis. These tissue- and stage-specific gene expressions of trehalase corresponded to the tissue- and stage-specificity of trehalase activity.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Feinberg A. P., Vogelstein B. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem. 1983 Jul 1;132(1):6–13. doi: 10.1016/0003-2697(83)90418-9. [DOI] [PubMed] [Google Scholar]
  2. Grimnes K. A., Bricker C. S., Happ G. M. Ordered flow of secretion from accessory glands to specific layers of the spermatophore of mealworm beetles: demonstration with a monoclonal antibody. J Exp Zool. 1986 Nov;240(2):275–286. doi: 10.1002/jez.1402400214. [DOI] [PubMed] [Google Scholar]
  3. Gutierrez C., Ardourel M., Bremer E., Middendorf A., Boos W., Ehmann U. Analysis and DNA sequence of the osmoregulated treA gene encoding the periplasmic trehalase of Escherichia coli K12. Mol Gen Genet. 1989 Jun;217(2-3):347–354. doi: 10.1007/BF02464903. [DOI] [PubMed] [Google Scholar]
  4. Happ G. M. Maturation of the male reproductive system and its endocrine regulation. Annu Rev Entomol. 1992;37:303–320. doi: 10.1146/annurev.en.37.010192.001511. [DOI] [PubMed] [Google Scholar]
  5. Henikoff S. Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencing. Gene. 1984 Jun;28(3):351–359. doi: 10.1016/0378-1119(84)90153-7. [DOI] [PubMed] [Google Scholar]
  6. Ruf J., Wacker H., James P., Maffia M., Seiler P., Galand G., von Kieckebusch A., Semenza G., Matei N. Rabbit small intestinal trehalase. Purification, cDNA cloning, expression, and verification of glycosylphosphatidylinositol anchoring. J Biol Chem. 1990 Sep 5;265(25):15034–15039. [PubMed] [Google Scholar]
  7. Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Shinbo H., Yaginuma T., Happ G. M. Purification and characterization of a proline-rich secretory protein that is a precursor to a structural protein of an insect spermatophore. J Biol Chem. 1987 Apr 5;262(10):4794–4799. [PubMed] [Google Scholar]
  9. Takesue Y., Yokota K., Miyajima S., Taguchi R., Ikezawa H. Membrane anchors of alkaline phosphatase and trehalase associated with the plasma membrane of larval midgut epithelial cells of the silkworm, Bombyx mori. J Biochem. 1989 Jun;105(6):998–1001. doi: 10.1093/oxfordjournals.jbchem.a122794. [DOI] [PubMed] [Google Scholar]
  10. Winograd I. J., Szabo B. J., Coplen T. B., Riggs A. C., Kolesar P. T. Two-million-year record of deuterium depletion in great basin ground waters. Science. 1985 Feb 1;227(4686):519–522. doi: 10.1126/science.227.4686.519. [DOI] [PubMed] [Google Scholar]
  11. Yaginuma T., Happ G. M. 20-Hydroxyecdysone acts in the male pupa to commit accessory glands toward trehalase production in the adult mealworm beetle (Tenebrio molitor). Gen Comp Endocrinol. 1989 Feb;73(2):173–185. doi: 10.1016/0016-6480(89)90090-7. [DOI] [PubMed] [Google Scholar]
  12. Yaginuma T., Happ G. M. Trehalase from the bean-shaped accessory glands and the spermatophore of the male mealworm beetle, Tenebrio molitor. J Comp Physiol B. 1988;157(6):765–770. doi: 10.1007/BF00691007. [DOI] [PubMed] [Google Scholar]
  13. von Heijne G. A new method for predicting signal sequence cleavage sites. Nucleic Acids Res. 1986 Jun 11;14(11):4683–4690. doi: 10.1093/nar/14.11.4683. [DOI] [PMC free article] [PubMed] [Google Scholar]

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