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. 1993 Jan 1;289(Pt 1):179–184. doi: 10.1042/bj2890179

Hyaluronate synthase: cloning and sequencing of the gene from Streptococcus sp.

M Lansing 1, S Lellig 1, A Mausolf 1, I Martini 1, F Crescenzi 1, M O'Regan 1, P Prehm 1
PMCID: PMC1132147  PMID: 8424756

Abstract

The complete nucleotide sequence of hyaluronate synthase from Streptococcus sp. and its flanking regions is presented. The gene locus was designated has. Southern-blotting results suggested that the gene was conserved in hyaluronate-producing streptococci. A putative translation-initiation codon was identified and the open reading frame consists of 1566 bp, specifying a protein of 56 kDa. Sequences resembling the promoter and ribosome-binding site of Gram-positive organisms are found upstream of the synthase. The predicted amino-acid sequence reveals the presence of a 35-residue signal peptide. The sequence has some similarity to bacterial peptide-binding proteins.

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Selected References

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