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. 1992 May 15;284(Pt 1):61–65. doi: 10.1042/bj2840061

Release of choline by phospholipase D and a related phosphoric diester hydrolase in human erythrocytes. 1H spin-echo n.m.r. studies.

H Selle 1, B E Chapman 1, P W Kuchel 1
PMCID: PMC1132697  PMID: 1318026

Abstract

A previously detected phosphatidylcholine-specific phospholipase D from lysates of human red blood cells has been further characterized by 1H spin-echo n.m.r. spectroscopy. A second choline-releasing enzymic activity was observed after addition of glycerophosphocholine. Both of these phosphoric diester hydrolase activities were activated to different extents by different concentrations of calcium ions. Differences between the two activities were also observed on inhibition by barium and phosphate ions. These distinct, choline-yielding, reactions which occur in the cytoplasm of red blood cells may be involved in the regulation of the levels of membrane phosphatidylcholine.

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Selected References

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  1. Beilharz G. R., Middlehurst C. R., Kuchel P. W., Hunt G. E., Johnson G. F. An experimental study and computer simulation of the turnover of choline in erythrocytes of patients treated with lithium carbonate. Aust J Exp Biol Med Sci. 1986 Jun;64(Pt 3):271–289. doi: 10.1038/icb.1986.29. [DOI] [PubMed] [Google Scholar]
  2. Berridge M. J. Inositol lipids and DNA replication. Philos Trans R Soc Lond B Biol Sci. 1987 Dec 15;317(1187):525–536. doi: 10.1098/rstb.1987.0079. [DOI] [PubMed] [Google Scholar]
  3. Berridge M. J. Inositol trisphosphate and diacylglycerol as second messengers. Biochem J. 1984 Jun 1;220(2):345–360. doi: 10.1042/bj2200345. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Berridge M. J., Irvine R. F. Inositol phosphates and cell signalling. Nature. 1989 Sep 21;341(6239):197–205. doi: 10.1038/341197a0. [DOI] [PubMed] [Google Scholar]
  5. Beutler E., West C., Blume K. G. The removal of leukocytes and platelets from whole blood. J Lab Clin Med. 1976 Aug;88(2):328–333. [PubMed] [Google Scholar]
  6. Billah M. M., Anthes J. C. The regulation and cellular functions of phosphatidylcholine hydrolysis. Biochem J. 1990 Jul 15;269(2):281–291. doi: 10.1042/bj2690281. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Brown F. F., Campbell I. D., Kuchel P. W., Rabenstein D. C. Human erythrocyte metabolism studies by 1H spin echo NMR. FEBS Lett. 1977 Oct 1;82(1):12–16. doi: 10.1016/0014-5793(77)80875-2. [DOI] [PubMed] [Google Scholar]
  8. Chapman B. E., Beilharz G. R., York M. J., Kuchel P. W. Endogenous phospholipase and choline release in human erythrocytes: a study using 1H-NMR spectroscopy. Biochem Biophys Res Commun. 1982 Apr 29;105(4):1280–1287. doi: 10.1016/0006-291x(82)90925-1. [DOI] [PubMed] [Google Scholar]
  9. DILS R. R., HUBSCHER G. Metabolism of phospholipids. III. The effect of calcium ions on the incorporation of labelled choline into rat-liver microsomes. Biochim Biophys Acta. 1961 Jan 29;46:505–513. doi: 10.1016/0006-3002(61)90581-9. [DOI] [PubMed] [Google Scholar]
  10. Exton J. H. Signaling through phosphatidylcholine breakdown. J Biol Chem. 1990 Jan 5;265(1):1–4. [PubMed] [Google Scholar]
  11. GOTTFRIED E. L., RAPPORT M. M. THE BIOCHEMISTRY OF PLASMALOGENS. II. HEMOLYTIC ACTIVITY OF SOME PLASMALOGEN DERIVATIVES. J Lipid Res. 1963 Jan;4:57–62. [PubMed] [Google Scholar]
  12. Gerritsen W. J., Henricks P. A., de Kruijff B., van Deenen L. L. The transbilayer movement of phosphatidylcholine in vesicles reconstituted with intrinsic proteins from the human erythrocyte membrane. Biochim Biophys Acta. 1980 Aug 14;600(3):607–619. doi: 10.1016/0005-2736(80)90464-2. [DOI] [PubMed] [Google Scholar]
  13. HUBSCHER G. Metabolism of phospholipids. VI. The effect of metal ions on the incorporation of L-serine into phosphatidylserine. Biochim Biophys Acta. 1962 Mar 12;57:555–561. doi: 10.1016/0006-3002(62)91163-0. [DOI] [PubMed] [Google Scholar]
  14. Irvine R. F., Hemington N., Dawson R. M. The hydrolysis of phosphatidylinositol by lysosomal enzymes of rat liver and brain. Biochem J. 1978 Nov 15;176(2):475–484. doi: 10.1042/bj1760475. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Jaffé E. R., Gottfried E. L. Hereditary nonspherocytic hemolytic disease associated with an altered phospholipid composition of the erythrocytes. J Clin Invest. 1968 Jun;47(6):1375–1388. doi: 10.1172/JCI105829. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Johnson G., Kuchel P. W. Red-cell choline in manic-depressive patients taking lithium. N Engl J Med. 1980 Sep 18;303(12):705–705. doi: 10.1056/NEJM198009183031223. [DOI] [PubMed] [Google Scholar]
  17. Jope R. S., Jenden D. J., Ehrlich B. E., Diamond J. M. Choline accumulates in erythrocytes during lithium therapy. N Engl J Med. 1978 Oct 12;299(15):833–834. [PubMed] [Google Scholar]
  18. Kater L. A., Goetzl E. J., Austen K. F. Isolation of human eosinophil phospholipase D. J Clin Invest. 1976 May;57(5):1173–1180. doi: 10.1172/JCI108385. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Mulder E., van Deenen L. L. Metabolism of red-cell lipids. 3. Pathways for phospholipid renewal. Biochim Biophys Acta. 1965 Oct 4;106(2):348–356. doi: 10.1016/0005-2760(65)90043-3. [DOI] [PubMed] [Google Scholar]
  20. ROBERTSON A. F., LANDS W. E. METABOLISM OF PHOSPHOLIPIDS IN NORMAL AND SPHEROCYTIC HUMAN ERYTHROCYTES. J Lipid Res. 1964 Jan;5:88–93. [PubMed] [Google Scholar]
  21. Reed C. F. Phospholipid exchange between plasma and erythrocytes in man and the dog. J Clin Invest. 1968 Apr;47(4):749–760. doi: 10.1172/JCI105770. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Reglinski J., Smith W. E., Sturrock R. D. Spin-echo 1H NMR detected response of ergothioneine to oxidative stress in the intact human erythrocyte. Magn Reson Med. 1988 Feb;6(2):217–223. doi: 10.1002/mrm.1910060210. [DOI] [PubMed] [Google Scholar]
  23. Richards D. E., Irvine R. F., Dawson R. M. Hydrolysis of membrane phospholipids by phospholipases of rat liver lysosomes. Biochem J. 1979 Aug 15;182(2):599–606. doi: 10.1042/bj1820599. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Saito M., Kanfer J. Phosphatidohydrolase activity in a solubilized preparation from rat brain particulate fraction. Arch Biochem Biophys. 1975 Jul;169(1):318–323. doi: 10.1016/0003-9861(75)90346-x. [DOI] [PubMed] [Google Scholar]
  25. Scherphof G. L., van Deenen L. L. On the pathways of fatty acid incorporation into the lipids of subcellular particles of rat liver and into erythrocytes. Biochim Biophys Acta. 1966 Feb 14;113(2):417–420. doi: 10.1016/s0926-6593(66)80087-5. [DOI] [PubMed] [Google Scholar]
  26. Shea P. A., Small J. G., Hendrie H. C. Elevation of choline and glycine in red blood cells of psychiatric patients due to lithium treatment. Biol Psychiatry. 1981 Sep;16(9):825–830. [PubMed] [Google Scholar]
  27. Shohet S. B. Hemolysis and changes in erythrocyte membrane lipids. I. N Engl J Med. 1972 Mar 16;286(11):577–583. doi: 10.1056/NEJM197203162861105. [DOI] [PubMed] [Google Scholar]
  28. Shohet S. B., Livermore B. M., Nathan D. G., Jaffé E. R. Hereditary hemolytic anemia associated with abnormal membrane lipids: mechanism of accumulation of phosphatidyl choline. Blood. 1971 Oct;38(4):445–456. [PubMed] [Google Scholar]
  29. Shohet S. B., Nathan D. G., Livermore B. M., Feig S. A., Jaffé E. R. Hereditary hemolytic anemia associated with abnormal membrane lipid. II. Ion permeability and transport abnormalities. Blood. 1973 Jul;42(1):1–8. [PubMed] [Google Scholar]
  30. Shohet S. B. Release of phospholipid fatty acid from human erythrocytes. J Clin Invest. 1970 Sep;49(9):1668–1678. doi: 10.1172/JCI106384. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Shohet S. B. The apparent transfer of fatty acid from phosphatidylcholine to phosphatidylethanolamine in human erythrocytes. J Lipid Res. 1971 Mar;12(2):139–142. [PubMed] [Google Scholar]
  32. Stoll A. L., Cohen B. M., Hanin I. Erythrocyte choline concentrations in psychiatric disorders. Biol Psychiatry. 1991 Feb 15;29(4):309–321. doi: 10.1016/0006-3223(91)90216-9. [DOI] [PubMed] [Google Scholar]
  33. Taki T., Kanfer J. N. Partial purification and properties of a rat brain phospholipase. J Biol Chem. 1979 Oct 10;254(19):9761–9765. [PubMed] [Google Scholar]
  34. Taylor C. W. The role of G proteins in transmembrane signalling. Biochem J. 1990 Nov 15;272(1):1–13. doi: 10.1042/bj2720001. [DOI] [PMC free article] [PubMed] [Google Scholar]
  35. Wolf R. A., Gross R. W. Identification of neutral active phospholipase C which hydrolyzes choline glycerophospholipids and plasmalogen selective phospholipase A2 in canine myocardium. J Biol Chem. 1985 Jun 25;260(12):7295–7303. [PubMed] [Google Scholar]
  36. Wood P. L., Suranyi-Cadotte B., Schwartz G., Nair N. P. Platelet 3H-imipramine binding and red blood cell choline in affective disorders: indications of heterogeneous pathogenesis. Biol Psychiatry. 1983 Jun;18(6):715–719. [PubMed] [Google Scholar]

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