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. 1992 Aug 1;285(Pt 3):791–797. doi: 10.1042/bj2850791

Overexpression of the erythrocyte plasma membrane Ca2+ pump in COS-1 cells.

H P Adamo 1, A K Verma 1, M A Sanders 1, R Heim 1, J L Salisbury 1, E D Wieben 1, J T Penniston 1
PMCID: PMC1132865  PMID: 1323273

Abstract

A full-length cDNA corresponding to the hPMCA4 plasma membrane Ca2+ pump was assembled and expressed in COS-1 cells. The original sequence of hPMCA4 gave a very low expression. The mutation of the initiation translation site of this sequence to the consensus A/G-X-X-AUG-G increased the production of the protein. The Ca2+ pump activity in transfected cells was 1.5-3.5-fold higher than in controls. The Ca(2+)-dependence and the calmodulin stimulation of hPMCA4 expressed in COS-1 cells were comparable with those of the erythrocyte Ca2+ pump. Immunohistochemistry experiments showed that most of the expressed protein remained in intracellular membranes. Possible explanations for this targeting of the pump are discussed.

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Selected References

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