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. 1989 Nov 1;263(3):965–968. doi: 10.1042/bj2630965

Adaptative decrease in expression of the mRNA for uncoupling protein and subunit II of cytochrome c oxidase in rat brown adipose tissue during pregnancy and lactation.

I Martin 1, M Giralt 1, O Viñas 1, R Iglesias 1, T Mampel 1, F Villarroya 1
PMCID: PMC1133525  PMID: 2557014

Abstract

Uncoupling-protein (UCP) mRNA expression is decreased to 15% of virgin control levels between days 10 and 15 of pregnancy, and remains at these low values during late pregnancy and lactation. Abrupt weaning of mid-lactating rats causes a slight but significant increase in UCP mRNA. Expression of mRNA for subunit II of cytochrome c oxidase (COII) decreased to half that of virgin control in late pregnancy and during lactation. Whereas COII mRNA expression is in step with the known modifications of brown-fat mitochondria content during the breeding cycle of the rat, UCP mRNA expression appears to be diminished much earlier than the mitochondrial proton-conductance-pathway activity. On the other hand, the reactivity of brown fat to increase expression of UCP and COII mRNAs in response to acute cold or noradrenaline treatment is not impaired during lactation.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bianco A. C., Silva J. E. Intracellular conversion of thyroxine to triiodothyronine is required for the optimal thermogenic function of brown adipose tissue. J Clin Invest. 1987 Jan;79(1):295–300. doi: 10.1172/JCI112798. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bouillaud F., Ricquier D., Thibault J., Weissenbach J. Molecular approach to thermogenesis in brown adipose tissue: cDNA cloning of the mitochondrial uncoupling protein. Proc Natl Acad Sci U S A. 1985 Jan;82(2):445–448. doi: 10.1073/pnas.82.2.445. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Falcou R., Bouillaud F., Mory G., Apfelbaum M., Ricquier D. Increase of uncoupling protein and its mRNA in brown adipose tissue of rats fed on 'cafeteria diet'. Biochem J. 1985 Oct 1;231(1):241–244. doi: 10.1042/bj2310241. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Glaichenhaus N., Léopold P., Cuzin F. Increased levels of mitochondrial gene expression in rat fibroblast cells immortalized or transformed by viral and cellular oncogenes. EMBO J. 1986 Jun;5(6):1261–1265. doi: 10.1002/j.1460-2075.1986.tb04355.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Isler D., Trayhurn P., Lunn P. G. Brown adipose tissue metabolism in lactating rats: the effect of litter size. Ann Nutr Metab. 1984;28(2):101–109. doi: 10.1159/000176789. [DOI] [PubMed] [Google Scholar]
  6. Jacobsson A., Nedergaard J., Cannon B. alpha- and beta-adrenergic control of thermogenin mRNA expression in brown adipose tissue. Biosci Rep. 1986 Jul;6(7):621–631. doi: 10.1007/BF01114756. [DOI] [PubMed] [Google Scholar]
  7. Lomedico P. T., Saunders G. F. Preparation of pancreatic mRNA: cell-free translation of an insulin-immunoreactive polypeptide. Nucleic Acids Res. 1976 Feb;3(2):381–391. doi: 10.1093/nar/3.2.381. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Mory G., Bouillaud F., Combes-George M., Ricquier D. Noradrenaline controls the concentration of the uncoupling protein in brown adipose tissue. FEBS Lett. 1984 Jan 30;166(2):393–396. doi: 10.1016/0014-5793(84)80120-9. [DOI] [PubMed] [Google Scholar]
  9. Reichling S., Ridley R. G., Patel H. V., Harley C. B., Freeman K. B. Loss of brown adipose tissue uncoupling protein mRNA on deacclimation of cold-exposed rats. Biochem Biophys Res Commun. 1987 Feb 13;142(3):696–701. doi: 10.1016/0006-291x(87)91470-7. [DOI] [PubMed] [Google Scholar]
  10. Ricquier D., Bouillaud F., Toumelin P., Mory G., Bazin R., Arch J., Pénicaud L. Expression of uncoupling protein mRNA in thermogenic or weakly thermogenic brown adipose tissue. Evidence for a rapid beta-adrenoreceptor-mediated and transcriptionally regulated step during activation of thermogenesis. J Biol Chem. 1986 Oct 25;261(30):13905–13910. [PubMed] [Google Scholar]
  11. Trayhurn P., Ashwell M., Jennings G., Richard D., Stirling D. M. Effect of warm or cold exposure on GDP binding and uncoupling protein in rat brown fat. Am J Physiol. 1987 Feb;252(2 Pt 1):E237–E243. doi: 10.1152/ajpendo.1987.252.2.E237. [DOI] [PubMed] [Google Scholar]
  12. Trayhurn P., Douglas J. B., McGuckin M. M. Brown adipose tissue thermogenesis is 'suppressed' during lactation in mice. Nature. 1982 Jul 1;298(5869):59–60. doi: 10.1038/298059a0. [DOI] [PubMed] [Google Scholar]
  13. Trayhurn P., Jennings G. Functional atrophy of brown adipose tissue during lactation in mice. Effects of lactation and weaning on mitochondrial GDP binding and uncoupling protein. Biochem J. 1987 Nov 15;248(1):273–276. doi: 10.1042/bj2480273. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Trayhurn P., Wusteman M. C. Sympathetic activity in brown adipose tissue in lactating mice. Am J Physiol. 1987 Nov;253(5 Pt 1):E515–E520. doi: 10.1152/ajpendo.1987.253.5.E515. [DOI] [PubMed] [Google Scholar]
  15. Villarroya F., Felipe A., Mampel T. Reduced noradrenaline turnover in brown adipose tissue of lactating rats. Comp Biochem Physiol A Comp Physiol. 1987;86(3):481–483. doi: 10.1016/0300-9629(87)90529-9. [DOI] [PubMed] [Google Scholar]
  16. Villarroya F., Felipe A., Mampel T. Sequential changes in brown adipose tissue composition, cytochrome oxidase activity and GDP binding throughout pregnancy and lactation in the rat. Biochim Biophys Acta. 1986 Jun 19;882(2):187–191. doi: 10.1016/0304-4165(86)90154-6. [DOI] [PubMed] [Google Scholar]
  17. Villarroya F., Mampel T. Changes in brown adipose tissue lipoprotein lipase activity and lipogenesis rate during pregnancy and lactation in the rat. Biochem Int. 1986 Sep;13(3):511–519. [PubMed] [Google Scholar]
  18. Viñas O., Giralt M., Obregón M. J., Iglesias R., Villarroya F., Mampel T. Iodothyronine 5'-deiodinase activity and thyroid hormone content in brown adipose tissue during the breeding cycle of the rat. Biochem J. 1988 Oct 15;255(2):457–461. doi: 10.1042/bj2550457. [DOI] [PMC free article] [PubMed] [Google Scholar]

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