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. 1990 Feb 1;265(3):899–902. doi: 10.1042/bj2650899

Chorismate synthase. Pre-steady-state kinetics of phosphate release from 5-enolpyruvylshikimate 3-phosphate.

T R Hawkes 1, T Lewis 1, J R Coggins 1, D M Mousdale 1, D J Lowe 1, R N Thorneley 1
PMCID: PMC1133717  PMID: 2407239

Abstract

The pre-steady-state kinetics of phosphate formation from 5-enolpyruvylshikimate 3-phosphate catalysed by Escherichia coli chorismate synthase (EC 4.6.1.4) were studied by a rapid-acid-quench technique at 25 degrees C at pH 7.5. No pre-steady-state 'burst' or 'lag' phase was observed, showing that phosphate is released concomitant with the rate-limiting step of the enzyme. The implications of this result for the mechanism of action of chorismate synthase are discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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