Skip to main content
PMC home page
Biochemical Journal logoLink to Biochemical Journal
. 1989 Jan 1;257(1):259–264. doi: 10.1042/bj2570259

The renal Na+/Ca2+ exchange system is located exclusively in the distal tubule.

C Ramachandran 1, M G Brunette 1
PMCID: PMC1135564  PMID: 2920016

Abstract

The movement of Ca2+ across the basolateral plasma membrane was determined in purified preparations of this membrane isolated from rabbit proximal and distal convoluted tubules. The ATP-dependent Ca2+ uptake was present in basolateral membranes from both these tubular segments, but the activity was higher in the distal tubules. A very active Na+/Ca2+ exchange system was also demonstrated in the distal-tubular membranes, but in proximal-tubular membranes this exchange system was not demonstrable. The presence of Na+ outside the vesicles gradually inhibited the ATP-dependent Ca2+ uptake in the distal-tubular-membrane preparations, but remained without effect in those from the proximal tubules. The activity of the Na+/Ca2+ exchange system in the distal-tubular membranes was a function of the imposed Na+ gradient. These results suggest that the major differences in the characteristics of Ca2+ transport in the proximal and in the distal tubules are due to the high activity of a Na+/Ca2+ exchange system in the distal tubule and its virtual absence in the proximal tubule.

Full text

PDF
260

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Agus Z. S., Gardner L. B., Beck L. H., Goldberg M. Effects of parathyroid hormone on renal tubular reabsorption of calcium, sodium, and phosphate. Am J Physiol. 1973 May;224(5):1143–1148. doi: 10.1152/ajplegacy.1973.224.5.1143. [DOI] [PubMed] [Google Scholar]
  2. Brunette M. G., Blouin S., Chan M. High affinity Ca2+ -Mg2+ ATPase in the distal tubule of the mouse kidney. Can J Physiol Pharmacol. 1987 Oct;65(10):2093–2098. doi: 10.1139/y87-328. [DOI] [PubMed] [Google Scholar]
  3. Brunette M. G., Chan M., Lebrun M. Phosphatase activity along the nephron of mice with hypophosphatemic vitamin-D-resistant rickets. Kidney Int. 1981 Aug;20(2):181–187. doi: 10.1038/ki.1981.120. [DOI] [PubMed] [Google Scholar]
  4. Brunette M., Aras M. A microinjection study of nephron permeability to calcium and magnesium. Am J Physiol. 1971 Nov;221(5):1442–1448. doi: 10.1152/ajplegacy.1971.221.5.1442. [DOI] [PubMed] [Google Scholar]
  5. Costanzo L. S., Windhager E. E. Calcium and sodium transport by the distal convoluted tubule of the rat. Am J Physiol. 1978 Nov;235(5):F492–F506. doi: 10.1152/ajprenal.1978.235.5.F492. [DOI] [PubMed] [Google Scholar]
  6. Costanzo L. S., Windhager E. E. Effects of PTH, ADH, and cyclic AMP on distal tubular Ca and Na reabsorption. Am J Physiol. 1980 Nov;239(5):F478–F485. doi: 10.1152/ajprenal.1980.239.5.F478. [DOI] [PubMed] [Google Scholar]
  7. Doucet A., Katz A. I. High-affinity Ca-Mg-ATPase along the rabbit nephron. Am J Physiol. 1982 Apr;242(4):F346–F352. doi: 10.1152/ajprenal.1982.242.4.F346. [DOI] [PubMed] [Google Scholar]
  8. Duarte C. G., Watson J. F. Calcium reabsorption in proximal tubule of the dog nephron. Am J Physiol. 1967 Jun;212(6):1355–1360. doi: 10.1152/ajplegacy.1967.212.6.1355. [DOI] [PubMed] [Google Scholar]
  9. Edwards B. R., Baer P. G., Sutton R. A., Dirks J. H. Micropuncture study of diuretic effects on sodium and calcium reabsorption in the dog nephron. J Clin Invest. 1973 Oct;52(10):2418–2427. doi: 10.1172/JCI107432. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Gesek F. A., Wolff D. W., Strandhoy J. W. Improved separation method for rat proximal and distal renal tubules. Am J Physiol. 1987 Aug;253(2 Pt 2):F358–F365. doi: 10.1152/ajprenal.1987.253.2.F358. [DOI] [PubMed] [Google Scholar]
  11. Gmaj P., Murer H., Carafoli E. Localization and properties of a high-affinity (Ca2+ + Mg2+)-ATPase in isolated kidney cortex plasma membranes. FEBS Lett. 1982 Aug 2;144(2):226–230. doi: 10.1016/0014-5793(82)80643-1. [DOI] [PubMed] [Google Scholar]
  12. Gmaj P., Murer H., Kinne R. Calcium ion transport across plasma membranes isolated from rat kidney cortex. Biochem J. 1979 Mar 15;178(3):549–557. doi: 10.1042/bj1780549. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Greger R., Lang F., Oberleithner H. Distal site of calcium reabsorption in the rat nephron. Pflugers Arch. 1978 May 18;374(2):153–157. doi: 10.1007/BF00581296. [DOI] [PubMed] [Google Scholar]
  14. Hanai H., Ishida M., Liang C. T., Sacktor B. Parathyroid hormone increases sodium/calcium exchange activity in renal cells and the blunting of the response in aging. J Biol Chem. 1986 Apr 25;261(12):5419–5425. [PubMed] [Google Scholar]
  15. Jayakumar A., Cheng L., Liang C. T., Sacktor B. Sodium gradient-dependent calcium uptake in renal basolateral membrane vesicles. Effect of parathyroid hormone. J Biol Chem. 1984 Sep 10;259(17):10827–10833. [PubMed] [Google Scholar]
  16. Kelly M. H., Hamilton J. R. A micro-technique for the assay of intestinal alkaline phosphatase. Results in normal children and in children with celiac disease. Clin Biochem. 1970 Mar;3(1):33–43. [PubMed] [Google Scholar]
  17. Khalifa S., Mills S., Hruska K. A. Stimulation of calcium uptake by parathyroid hormone in renal brush-border membrane vesicles. Relationship to membrane phosphorylation. J Biol Chem. 1983 Dec 10;258(23):14400–14406. [PubMed] [Google Scholar]
  18. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  19. Le Grimellec C., Roinel N., Morel F. Simultaneous Mg, Ca, P,K,Na and Cl analysis in rat tubular fluid. I. During perfusion of either inulin or ferrocyanide. Pflugers Arch. 1973 May 23;340(3):181–196. doi: 10.1007/BF00586838. [DOI] [PubMed] [Google Scholar]
  20. Levy J., Gavin J. R., 3rd, Morimoto S., Hammerman M. R., Avioli L. V. Hormonal regulation of (Ca2+ + Mg2+)ATPase activity in canine renal basolateral membrane. Endocrinology. 1986 Dec;119(6):2405–2411. doi: 10.1210/endo-119-6-2405. [DOI] [PubMed] [Google Scholar]
  21. Murayama Y., Morel F., Le Grimellec C. Phosphate, calcium and magnesium transfers in proximal tubules and loops of Henle, as measured by single nephron microperfusion experiments in the rat. Pflugers Arch. 1972;333(1):1–16. doi: 10.1007/BF00586037. [DOI] [PubMed] [Google Scholar]
  22. Ng R. C., Rouse D., Suki W. N. Calcium transport in the rabbit superficial proximal convoluted tubule. J Clin Invest. 1984 Sep;74(3):834–842. doi: 10.1172/JCI111500. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Scoble J. E., Mills S., Hruska K. A. Calcium transport in canine renal basolateral membrane vesicles. Effects of parathyroid hormone. J Clin Invest. 1985 Apr;75(4):1096–1105. doi: 10.1172/JCI111803. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Shareghi G. R., Stoner L. C. Calcium transport across segments of the rabbit distal nephron in vitro. Am J Physiol. 1978 Oct;235(4):F367–F375. doi: 10.1152/ajprenal.1978.235.4.F367. [DOI] [PubMed] [Google Scholar]
  25. Sutton R. A., Wong N. L., Dirks J. H. Effects of parathyroid hormone on sodium and calcium transport in the dog nephron. Clin Sci Mol Med. 1976 Oct;51(4):345–351. doi: 10.1042/cs0510345. [DOI] [PubMed] [Google Scholar]
  26. Ullrich K. J., Capasso G., Rumrich G., Papavassiliou F., Klöss S. Coupling between proximal tubular transport processes. Studies with ouabain, SITS and HCO3-free solutions. Pflugers Arch. 1977 Apr 25;368(3):245–252. doi: 10.1007/BF00585203. [DOI] [PubMed] [Google Scholar]
  27. Ullrich K. J., Rumrich G., Klöss S. Active Ca2+ reabsorption in the proximal tubule of the rat kidney. Dependence on sodium- and buffer transport. Pflugers Arch. 1976 Aug 24;364(3):223–228. doi: 10.1007/BF00581759. [DOI] [PubMed] [Google Scholar]
  28. Vieyra A., Nachbin L., de Dios-Abad E., Goldfeld M., Meyer-Fernandes J. R., de Moraes L. Comparison between calcium transport and adenosine triphosphatase activity in membrane vesicles derived from rabbit kidney proximal tubules. J Biol Chem. 1986 Mar 25;261(9):4247–4255. [PubMed] [Google Scholar]
  29. Vinay P., Gougoux A., Lemieux G. Isolation of a pure suspension of rat proximal tubules. Am J Physiol. 1981 Oct;241(4):F403–F411. doi: 10.1152/ajprenal.1981.241.4.F403. [DOI] [PubMed] [Google Scholar]
  30. van Heeswijk M. P., Geertsen J. A., van Os C. H. Kinetic properties of the ATP-dependent Ca2+ pump and the Na+/Ca2+ exchange system in basolateral membranes from rat kidney cortex. J Membr Biol. 1984;79(1):19–31. doi: 10.1007/BF01868523. [DOI] [PubMed] [Google Scholar]
  31. van Os C. H. Transcellular calcium transport in intestinal and renal epithelial cells. Biochim Biophys Acta. 1987 Jun 24;906(2):195–222. doi: 10.1016/0304-4157(87)90012-8. [DOI] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES