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. 1989 Jan 15;257(2):599–602. doi: 10.1042/bj2570599

Incorporation of atmospheric oxygen into the carbonyl functionality of the protochlorophyllide isocyclic ring.

C J Walker 1, K E Mansfield 1, K M Smith 1, P A Castelfranco 1
PMCID: PMC1135620  PMID: 2930469

Abstract

Detached cucumber (Cucumis sativus L. var. Beit Alpha) cotyledons incubated in darkness with 5-aminolaevulinic acid and either 16O2 air (control) or 18O2 in N2 accumulated protochlorophyllide. This was converted into methyl phaeoporphyrin alpha 5 and analysed by mass spectrometry. The molecular ion of the methyl phaeoporphyrin alpha 5 derived from the 18O2 incubation was 2 mass units greater than that of the control, establishing that the oxo oxygen atom of the isocyclic ring is derived from atmospheric oxygen.

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Selected References

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