Skip to main content
PMC home page
Biochemical Journal logoLink to Biochemical Journal
. 1995 Oct 1;311(Pt 1):41–44. doi: 10.1042/bj3110041

Putative capacitative calcium entry channels: expression of Drosophila trp and evidence for the existence of vertebrate homologues.

C C Petersen 1, M J Berridge 1, M F Borgese 1, D L Bennett 1
PMCID: PMC1136116  PMID: 7575478

Abstract

Capacitative calcium entry is a major pathway through which intracellular calcium stores are refilled after stimulation. It has been suggested that the protein encoded by the transient receptor potential (trp) gene expressed in Drosophila photoreceptors may be homologous with capacitative calcium entry channels. Expression of the trp gene product in Xenopus oocytes led to significant increases in calcium entry only when the intracellular calcium stores were depleted. Previous investigations have found trp to be uniquely expressed in Drosophila photoreceptors, but PCR cloning shows that homologous proteins exist in Calliphora, mouse brain and Xenopus oocytes. It is thus possible that capacitative calcium entry in Xenopus oocytes is mediated by a homologue of trp.

Full text

PDF
41

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Berridge M. J. Inositol trisphosphate and calcium signalling. Nature. 1993 Jan 28;361(6410):315–325. doi: 10.1038/361315a0. [DOI] [PubMed] [Google Scholar]
  2. Clapham D. E. Calcium signaling. Cell. 1995 Jan 27;80(2):259–268. doi: 10.1016/0092-8674(95)90408-5. [DOI] [PubMed] [Google Scholar]
  3. Hardie R. C., Minke B. Novel Ca2+ channels underlying transduction in Drosophila photoreceptors: implications for phosphoinositide-mediated Ca2+ mobilization. Trends Neurosci. 1993 Sep;16(9):371–376. doi: 10.1016/0166-2236(93)90095-4. [DOI] [PubMed] [Google Scholar]
  4. Hoth M., Penner R. Depletion of intracellular calcium stores activates a calcium current in mast cells. Nature. 1992 Jan 23;355(6358):353–356. doi: 10.1038/355353a0. [DOI] [PubMed] [Google Scholar]
  5. Hu Y., Schilling W. P. Receptor-mediated activation of recombinant Trpl expressed in Sf9 insect cells. Biochem J. 1995 Jan 15;305(Pt 2):605–611. doi: 10.1042/bj3050605. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hu Y., Vaca L., Zhu X., Birnbaumer L., Kunze D. L., Schilling W. P. Appearance of a novel Ca2+ influx pathway in Sf9 insect cells following expression of the transient receptor potential-like (trpl) protein of Drosophila. Biochem Biophys Res Commun. 1994 Jun 15;201(2):1050–1056. doi: 10.1006/bbrc.1994.1808. [DOI] [PubMed] [Google Scholar]
  7. Lupu-Meiri M., Beit-Or A., Christensen S. B., Oron Y. Calcium entry in Xenopus oocytes: effects of inositol trisphosphate, thapsigargin and DMSO. Cell Calcium. 1993 Feb;14(2):101–110. doi: 10.1016/0143-4160(93)90080-p. [DOI] [PubMed] [Google Scholar]
  8. Montell C., Rubin G. M. Molecular characterization of the Drosophila trp locus: a putative integral membrane protein required for phototransduction. Neuron. 1989 Apr;2(4):1313–1323. doi: 10.1016/0896-6273(89)90069-x. [DOI] [PubMed] [Google Scholar]
  9. Parekh A. B., Terlau H., Stühmer W. Depletion of InsP3 stores activates a Ca2+ and K+ current by means of a phosphatase and a diffusible messenger. Nature. 1993 Aug 26;364(6440):814–818. doi: 10.1038/364814a0. [DOI] [PubMed] [Google Scholar]
  10. Penner R., Fasolato C., Hoth M. Calcium influx and its control by calcium release. Curr Opin Neurobiol. 1993 Jun;3(3):368–374. doi: 10.1016/0959-4388(93)90130-q. [DOI] [PubMed] [Google Scholar]
  11. Petersen C. C., Berridge M. J. G-protein regulation of capacitative calcium entry may be mediated by protein kinases A and C in Xenopus oocytes. Biochem J. 1995 May 1;307(Pt 3):663–668. doi: 10.1042/bj3070663. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Petersen C. C., Berridge M. J. The regulation of capacitative calcium entry by calcium and protein kinase C in Xenopus oocytes. J Biol Chem. 1994 Dec 23;269(51):32246–32253. [PubMed] [Google Scholar]
  13. Phillips A. M., Bull A., Kelly L. E. Identification of a Drosophila gene encoding a calmodulin-binding protein with homology to the trp phototransduction gene. Neuron. 1992 Apr;8(4):631–642. doi: 10.1016/0896-6273(92)90085-r. [DOI] [PubMed] [Google Scholar]
  14. Pollock J. A., Assaf A., Peretz A., Nichols C. D., Mojet M. H., Hardie R. C., Minke B. TRP, a protein essential for inositide-mediated Ca2+ influx is localized adjacent to the calcium stores in Drosophila photoreceptors. J Neurosci. 1995 May;15(5 Pt 2):3747–3760. doi: 10.1523/JNEUROSCI.15-05-03747.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Putney J. W., Jr, Bird G. S. The signal for capacitative calcium entry. Cell. 1993 Oct 22;75(2):199–201. doi: 10.1016/0092-8674(93)80061-i. [DOI] [PubMed] [Google Scholar]
  16. Putney J. W., Jr Capacitative calcium entry revisited. Cell Calcium. 1990 Nov-Dec;11(10):611–624. doi: 10.1016/0143-4160(90)90016-n. [DOI] [PubMed] [Google Scholar]
  17. Vaca L., Sinkins W. G., Hu Y., Kunze D. L., Schilling W. P. Activation of recombinant trp by thapsigargin in Sf9 insect cells. Am J Physiol. 1994 Nov;267(5 Pt 1):C1501–C1505. doi: 10.1152/ajpcell.1994.267.5.C1501. [DOI] [PubMed] [Google Scholar]
  18. Wong F., Schaefer E. L., Roop B. C., LaMendola J. N., Johnson-Seaton D., Shao D. Proper function of the Drosophila trp gene product during pupal development is important for normal visual transduction in the adult. Neuron. 1989 Jul;3(1):81–94. doi: 10.1016/0896-6273(89)90117-7. [DOI] [PubMed] [Google Scholar]
  19. Zweifach A., Lewis R. S. Mitogen-regulated Ca2+ current of T lymphocytes is activated by depletion of intracellular Ca2+ stores. Proc Natl Acad Sci U S A. 1993 Jul 1;90(13):6295–6299. doi: 10.1073/pnas.90.13.6295. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES