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. 1994 Aug 15;302(Pt 1):5–9. doi: 10.1042/bj3020005

Receptor-mediated Mn2+ influx in rat hepatocytes: comparison of cells loaded with Fura-2 ester and cells microinjected with Fura-2 salt.

G E Kass 1, D L Webb 1, S C Chow 1, J Llopis 1, P O Berggren 1
PMCID: PMC1137183  PMID: 8068023

Abstract

In single Fura-2 ester-loaded hepatocytes, stimulation by vasopressin, but not emptying of the agonist-sensitive Ca2+ store by 2,5-di-(t-butyl)hydroquinone, resulted in an increase in the rate of Fura-2 fluorescence-quenching by Mn2+. Similarly, in cells microinjected with Fura-2 salt, vasopressin stimulated Mn2+ entry while 2,5-di-(t-butyl)hydroquinone or thapsigargin did not. The pattern of Fura-2 quenching by Mn2+ only correlated with the movement of Mn2+ across the plasma membrane.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arends M. J., Wyllie A. H. Apoptosis: mechanisms and roles in pathology. Int Rev Exp Pathol. 1991;32:223–254. doi: 10.1016/b978-0-12-364932-4.50010-1. [DOI] [PubMed] [Google Scholar]
  2. Berridge M. J. Inositol trisphosphate and calcium signalling. Nature. 1993 Jan 28;361(6410):315–325. doi: 10.1038/361315a0. [DOI] [PubMed] [Google Scholar]
  3. Bird G. S., Putney J. W., Jr Inhibition of thapsigargin-induced calcium entry by microinjected guanine nucleotide analogues. Evidence for the involvement of a small G-protein in capacitative calcium entry. J Biol Chem. 1993 Oct 15;268(29):21486–21488. [PubMed] [Google Scholar]
  4. Byron K. L., Babnigg G., Villereal M. L. Bradykinin-induced Ca2+ entry, release, and refilling of intracellular Ca2+ stores. Relationships revealed by image analysis of individual human fibroblasts. J Biol Chem. 1992 Jan 5;267(1):108–118. [PubMed] [Google Scholar]
  5. Chow S. C., Kass G. E., Orrenius S. Two independently regulated Ca2+ entry mechanisms coexist in Jurkat T cells during T cell receptor antigen activation. Biochem J. 1993 Jul 15;293(Pt 2):395–398. doi: 10.1042/bj2930395. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Clementi E., Scheer H., Zacchetti D., Fasolato C., Pozzan T., Meldolesi J. Receptor-activated Ca2+ influx. Two independently regulated mechanisms of influx stimulation coexist in neurosecretory PC12 cells. J Biol Chem. 1992 Feb 5;267(4):2164–2172. [PubMed] [Google Scholar]
  7. Collins M. K., Lopez Rivas A. The control of apoptosis in mammalian cells. Trends Biochem Sci. 1993 Aug;18(8):307–309. doi: 10.1016/0968-0004(93)90042-l. [DOI] [PubMed] [Google Scholar]
  8. Connor J. A. Intracellular calcium mobilization by inositol 1,4,5-trisphosphate: intracellular movements and compartmentalization. Cell Calcium. 1993 Mar;14(3):185–200. doi: 10.1016/0143-4160(93)90066-f. [DOI] [PubMed] [Google Scholar]
  9. Ellis R. E., Yuan J. Y., Horvitz H. R. Mechanisms and functions of cell death. Annu Rev Cell Biol. 1991;7:663–698. doi: 10.1146/annurev.cb.07.110191.003311. [DOI] [PubMed] [Google Scholar]
  10. Ely J. A., Ambroz C., Baukal A. J., Christensen S. B., Balla T., Catt K. J. Relationship between agonist- and thapsigargin-sensitive calcium pools in adrenal glomerulosa cells. Thapsigargin-induced Ca2+ mobilization and entry. J Biol Chem. 1991 Oct 5;266(28):18635–18641. [PubMed] [Google Scholar]
  11. Fasolato C., Hoth M., Penner R. A GTP-dependent step in the activation mechanism of capacitative calcium influx. J Biol Chem. 1993 Oct 5;268(28):20737–20740. [PubMed] [Google Scholar]
  12. Fasolato C., Hoth M., Penner R. Multiple mechanisms of manganese-induced quenching of fura-2 fluorescence in rat mast cells. Pflugers Arch. 1993 May;423(3-4):225–231. doi: 10.1007/BF00374399. [DOI] [PubMed] [Google Scholar]
  13. Glennon M. C., Bird G. S., Kwan C. Y., Putney J. W., Jr Actions of vasopressin and the Ca(2+)-ATPase inhibitor, thapsigargin, on Ca2+ signaling in hepatocytes. J Biol Chem. 1992 Apr 25;267(12):8230–8233. [PubMed] [Google Scholar]
  14. Hajnóczky G., Gao E., Nomura T., Hoek J. B., Thomas A. P. Multiple mechanisms by which protein kinase A potentiates inositol 1,4,5-trisphosphate-induced Ca2+ mobilization in permeabilized hepatocytes. Biochem J. 1993 Jul 15;293(Pt 2):413–422. doi: 10.1042/bj2930413. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Hallam T. J., Rink T. J. Agonists stimulate divalent cation channels in the plasma membrane of human platelets. FEBS Lett. 1985 Jul 8;186(2):175–179. doi: 10.1016/0014-5793(85)80703-1. [DOI] [PubMed] [Google Scholar]
  16. Kass G. E., Duddy S. K., Moore G. A., Orrenius S. 2,5-Di-(tert-butyl)-1,4-benzohydroquinone rapidly elevates cytosolic Ca2+ concentration by mobilizing the inositol 1,4,5-trisphosphate-sensitive Ca2+ pool. J Biol Chem. 1989 Sep 15;264(26):15192–15198. [PubMed] [Google Scholar]
  17. Kass G. E., Llopis J., Chow S. C., Duddy S. K., Orrenius S. Receptor-operated calcium influx in rat hepatocytes. Identification and characterization using manganese. J Biol Chem. 1990 Oct 15;265(29):17486–17492. [PubMed] [Google Scholar]
  18. Kawanishi T., Blank L. M., Harootunian A. T., Smith M. T., Tsien R. Y. Ca2+ oscillations induced by hormonal stimulation of individual fura-2-loaded hepatocytes. J Biol Chem. 1989 Aug 5;264(22):12859–12866. [PubMed] [Google Scholar]
  19. Lemasters J. J., Gores G. J., Nieminen A. L., Dawson T. L., Wray B. E., Herman B. Multiparameter digitized video microscopy of toxic and hypoxic injury in single cells. Environ Health Perspect. 1990 Mar;84:83–94. doi: 10.1289/ehp.908483. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Llopis J., Chow S. B., Kass G. E., Gahm A., Orrenius S. Comparison between the effects of the microsomal Ca(2+)-translocase inhibitors thapsigargin and 2,5-di-(t-butyl)-1,4-benzohydroquinone on cellular calcium fluxes. Biochem J. 1991 Jul 15;277(Pt 2):553–556. doi: 10.1042/bj2770553. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Llopis J., Kass G. E., Gahm A., Orrenius S. Evidence for two pathways of receptor-mediated Ca2+ entry in hepatocytes. Biochem J. 1992 May 15;284(Pt 1):243–247. doi: 10.1042/bj2840243. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Merritt J. E., Jacob R., Hallam T. J. Use of manganese to discriminate between calcium influx and mobilization from internal stores in stimulated human neutrophils. J Biol Chem. 1989 Jan 25;264(3):1522–1527. [PubMed] [Google Scholar]
  23. Nathanson M. H., Burgstahler A. D. Subcellular distribution of cytosolic Ca2+ in isolated rat hepatocyte couplets: evaluation using confocal microscopy. Cell Calcium. 1992 Feb;13(2):89–98. doi: 10.1016/0143-4160(92)90002-a. [DOI] [PubMed] [Google Scholar]
  24. Parekh A. B., Terlau H., Stühmer W. Depletion of InsP3 stores activates a Ca2+ and K+ current by means of a phosphatase and a diffusible messenger. Nature. 1993 Aug 26;364(6440):814–818. doi: 10.1038/364814a0. [DOI] [PubMed] [Google Scholar]
  25. Perotti M., Toddei F., Mirabelli F., Vairetti M., Bellomo G., McConkey D. J., Orrenius S. Calcium-dependent DNA fragmentation in human synovial cells exposed to cold shock. FEBS Lett. 1990 Jan 1;259(2):331–334. doi: 10.1016/0014-5793(90)80040-p. [DOI] [PubMed] [Google Scholar]
  26. Putney J. W., Jr A model for receptor-regulated calcium entry. Cell Calcium. 1986 Feb;7(1):1–12. doi: 10.1016/0143-4160(86)90026-6. [DOI] [PubMed] [Google Scholar]
  27. Putney J. W., Jr Capacitative calcium entry revisited. Cell Calcium. 1990 Nov-Dec;11(10):611–624. doi: 10.1016/0143-4160(90)90016-n. [DOI] [PubMed] [Google Scholar]
  28. Randriamampita C., Tsien R. Y. Emptying of intracellular Ca2+ stores releases a novel small messenger that stimulates Ca2+ influx. Nature. 1993 Aug 26;364(6440):809–814. doi: 10.1038/364809a0. [DOI] [PubMed] [Google Scholar]
  29. Soloff B. L., Nagle W. A., Moss A. J., Jr, Henle K. J., Crawford J. T. Apoptosis induced by cold shock in vitro is dependent on cell growth phase. Biochem Biophys Res Commun. 1987 Jun 15;145(2):876–883. doi: 10.1016/0006-291x(87)91046-1. [DOI] [PubMed] [Google Scholar]
  30. Stauderman K. A., Pruss R. M. Dissociation of Ca2+ entry and Ca2+ mobilization responses to angiotensin II in bovine adrenal chromaffin cells. J Biol Chem. 1989 Nov 5;264(31):18349–18355. [PubMed] [Google Scholar]
  31. Steinberg S. F., Bilezikian J. P., Al-Awqati Q. Fura-2 fluorescence is localized to mitochondria in endothelial cells. Am J Physiol. 1987 Nov;253(5 Pt 1):C744–C747. doi: 10.1152/ajpcell.1987.253.5.C744. [DOI] [PubMed] [Google Scholar]
  32. Striggow F., Bohnensack R. Verapamil and diltiazem inhibit receptor-operated calcium channels and intracellular calcium oscillations in rat hepatocytes. FEBS Lett. 1993 Mar 8;318(3):341–344. doi: 10.1016/0014-5793(93)80542-3. [DOI] [PubMed] [Google Scholar]

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