Skip to main content
PMC home page
Biochemical Journal logoLink to Biochemical Journal
. 1994 Dec 15;304(Pt 3):781–786. doi: 10.1042/bj3040781

Incorporation of carbon from photosynthetic products into 2-carboxyarabinitol-1-phosphate and 2-carboxyarabinitol.

P J Andralojc 1, G W Dawson 1, M A Parry 1, A J Keys 1
PMCID: PMC1137402  PMID: 7818481

Abstract

The synthesis of 2-carboxy-D-arabinitol-1-phosphate (CA1P), the naturally occurring inhibitor of ribulose-1,5-bisphosphate carboxylase/oxygenase, was studied in leaves of the French bean Phaseolus vulgaris, L. Leaves were supplied with air containing 14CO2 in the light then the plants were transferred to normal air in the light or in the dark. Leaf samples were frozen in liquid nitrogen, ground to a powder and extracted with acid. Lipids, pigments and cations were removed from the extract and CA1P and 2-carboxy-D-arabinitol (CA) recovered by anion exchange chromatography. The CA1P was further purified by its specific binding to purified ribulose-1,5-bisphosphate carboxylase/oxygenase. CA and CA1P were identified by chromatographic properties and n.m.r. spectra. When plants were kept for 15 h in darkness after exposure to 14CO2, up to 2.2% and 5.5% of the radioactivity in the extracts was present in CA1P and CA, respectively. The most radioactivity appeared in these compounds when photosynthesis from 14CO2 took place at low photosynthetic photon flux density (PPFD). Under such conditions, radioactivity was detected in CA1P after only 10 min. During subsequent exposure to normal air (12CO2) at low PPFD the amount of radioactivity in CA1P remained almost constant for 6 h; in darkness the rate of incorporation of radioactivity into CA1P reached a maximum after 2 h and the radioactivity was still increasing 6 h later. At low PPFD, the amount of CA1P in the leaves reached a maximum after 2 h. In darkness, the amount of CA1P began to increase rapidly after a lag of almost 1 h, well ahead of the increase in radioactivity in CA1P.

Full text

PDF
781

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Beck E., Stransky H., Fürbringer M. Synthesis of hamamelose-diphosphate by isolated spinach chloroplasts. FEBS Lett. 1971 Mar 16;13(4):229–234. doi: 10.1016/0014-5793(71)80542-2. [DOI] [PubMed] [Google Scholar]
  2. Berry J. A., Lorimer G. H., Pierce J., Seemann J. R., Meek J., Freas S. Isolation, identification, and synthesis of 2-carboxyarabinitol 1-phosphate, a diurnal regulator of ribulose-bisphosphate carboxylase activity. Proc Natl Acad Sci U S A. 1987 Feb;84(3):734–738. doi: 10.1073/pnas.84.3.734. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Holbrook G. P., Bowes G., Salvucci M. E. Degradation of 2-carboxyarabinitol 1-phosphate by a specific chloroplast phosphatase. Plant Physiol. 1989 Jun;90(2):673–678. doi: 10.1104/pp.90.2.673. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Isherwood F. A., Barrett F. C. Analysis of phosphate esters in plant material. Extraction and purification. Biochem J. 1967 Sep;104(3):922–933. doi: 10.1042/bj1040922. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Kingston-Smith A. H., Major I., Parry M. A., Keys A. J. Purification and properties of a phosphatase in French bean (Phaseolus vulgaris L.) leaves that hydrolyses 2'-carboxy-D-arabinitol 1-phosphate. Biochem J. 1992 Nov 1;287(Pt 3):821–825. doi: 10.1042/bj2870821. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Kobza J., Seemann J. R. Light-dependent kinetics of 2-carboxyarabinitol 1-phosphate metabolism and ribulose-1,5-bisphosphate carboxylase activity in vivo. Plant Physiol. 1989 Jan;89(1):174–179. doi: 10.1104/pp.89.1.174. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Moore B. D., Kobza J., Seemann J. R. Measurement of 2-carboxyarabinitol 1-phosphate in plant leaves by isotope dilution. Plant Physiol. 1991 May;96(1):208–213. doi: 10.1104/pp.96.1.208. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Moore B. D., Seemann J. R. Metabolism of 2'-carboxyarabinitol in leaves. Plant Physiol. 1992 Aug;99(4):1551–1555. doi: 10.1104/pp.99.4.1551. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Moore B. D., Sharkey T. D., Kobza J., Seemann J. R. Identification and levels of 2'-carboxyarabinitol in leaves. Plant Physiol. 1992 Aug;99(4):1546–1550. doi: 10.1104/pp.99.4.1546. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Paulsen J. M., Lane M. D. Spinach ribulose diphosphate carboxylase. I. Purification and properties of the enzyme. Biochemistry. 1966 Jul;5(7):2350–2357. doi: 10.1021/bi00871a025. [DOI] [PubMed] [Google Scholar]
  11. Salvucci M. E., Holbrook G. P. Purification and Properties of 2-Carboxy-d-Arabinitol 1-Phosphatase. Plant Physiol. 1989 Jun;90(2):679–685. doi: 10.1104/pp.90.2.679. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Vu C. V., Allen L. H., Bowes G. Effects of Light and Elevated Atmospheric CO(2) on the Ribulose Bisphosphate Carboxylase Activity and Ribulose Bisphosphate Level of Soybean Leaves. Plant Physiol. 1983 Nov;73(3):729–734. doi: 10.1104/pp.73.3.729. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES