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. 1994 Nov 15;304(Pt 1):57–60. doi: 10.1042/bj3040057

Thapsigargin-induced nuclear calcium signals in rat basophilic leukaemia cells.

Y Horikoshi 1, T Furuno 1, R Teshima 1, J Sawada 1, M Nakanishi 1
PMCID: PMC1137451  PMID: 7998957

Abstract

By a confocal fluorescence microscope with an argon-ion laser (488 nm) and a He-Cd laser (325 nm) we have studied thapsigargin-induced calcium signals in individual rat basophilic leukaemia (RBL-2H3) cells. In the presence or absence of external calcium ions, thapsigargin-induced calcium signals were transferred to the nucleus as well as to the cytoplasm of RBL-2H3 cells. The calcium signals were generally much stronger in the nucleus than in the cytoplasm. However, some of the RBL-2H3 cells had apparently reduced nuclear calcium signals. They had a basophil-like bilobed (multilobed) nucleus, although most RBL-2H3 cells had a mast-cell-like monolobed nucleus. In the cells with a bilobed nucleus, IgE-receptor-mediated calcium signals were neither transferred to the nucleus nor to the cytoplasm. The results gave a new insight into the understanding of the mechanism of the nuclear calcium signals in RBL-2H3 cells.

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Selected References

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  1. Arndt-Jovin D. J., Jovin T. M. Analysis and sorting of living cells according to deoxyribonucleic acid content. J Histochem Cytochem. 1977 Jul;25(7):585–589. doi: 10.1177/25.7.70450. [DOI] [PubMed] [Google Scholar]
  2. Barsumian E. L., Isersky C., Petrino M. G., Siraganian R. P. IgE-induced histamine release from rat basophilic leukemia cell lines: isolation of releasing and nonreleasing clones. Eur J Immunol. 1981 Apr;11(4):317–323. doi: 10.1002/eji.1830110410. [DOI] [PubMed] [Google Scholar]
  3. Basciano L. K., Berenstein E. H., Kmak L., Siraganian R. P. Monoclonal antibodies that inhibit IgE binding. J Biol Chem. 1986 Sep 5;261(25):11823–11831. [PubMed] [Google Scholar]
  4. Berridge M. J. Inositol trisphosphate and calcium signalling. Nature. 1993 Jan 28;361(6410):315–325. doi: 10.1038/361315a0. [DOI] [PubMed] [Google Scholar]
  5. Böttcher I., Ulrich M., Hirayama N., Ovary Z. Production of monoclonal mouse IgE antibodies with DNP specificity by hybrid cell lines. Int Arch Allergy Appl Immunol. 1980;61(2):248–250. doi: 10.1159/000232441. [DOI] [PubMed] [Google Scholar]
  6. Dar O., Pecht I. Fc epsilon receptor mediated Ca2+ influx into mast cells is modulated by the concentration of cytosolic free Ca2+ ions. FEBS Lett. 1992 Sep 28;310(2):123–128. doi: 10.1016/0014-5793(92)81311-9. [DOI] [PubMed] [Google Scholar]
  7. Furuno T., Hamano T., Nakanishi M. Receptor-mediated calcium signal playing a nuclear third messenger in the activation of antigen-specific B cells. Biophys J. 1993 Mar;64(3):665–669. doi: 10.1016/S0006-3495(93)81425-5. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Grynkiewicz G., Poenie M., Tsien R. Y. A new generation of Ca2+ indicators with greatly improved fluorescence properties. J Biol Chem. 1985 Mar 25;260(6):3440–3450. [PubMed] [Google Scholar]
  9. Ishizaka T., Sterk A. R., Daeron M., Becker E. L., Ishizaka K. Biochemical analysis of desensitization of mouse mast cells. J Immunol. 1985 Jul;135(1):492–501. [PubMed] [Google Scholar]
  10. Kato K., Nakanishi M., Arata Y., Teshima R., Terao T., Miyamoto H. Calcium influx in a single rat basophilic leukemia cell as revealed with a digital imaging fluorescence microscope. J Biochem. 1987 Jul;102(1):1–4. doi: 10.1093/oxfordjournals.jbchem.a122020. [DOI] [PubMed] [Google Scholar]
  11. Kato K., Nakanishi M., Arata Y., Teshima R., Terao T., Miyamoto H., Hanaoka F., Ui M., Masuzawa Y., Hiraishi S. Characterization of a new type of variant of rat basophilic leukemia 2H3 cells presenting a different pattern of calcium signal. Exp Cell Res. 1990 Jun;188(2):247–253. doi: 10.1016/0014-4827(90)90166-8. [DOI] [PubMed] [Google Scholar]
  12. Millard P. J., Gross D., Webb W. W., Fewtrell C. Imaging asynchronous changes in intracellular Ca2+ in individual stimulated tumor mast cells. Proc Natl Acad Sci U S A. 1988 Mar;85(6):1854–1858. doi: 10.1073/pnas.85.6.1854. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Millard P. J., Ryan T. A., Webb W. W., Fewtrell C. Immunoglobulin E receptor cross-linking induces oscillations in intracellular free ionized calcium in individual tumor mast cells. J Biol Chem. 1989 Nov 25;264(33):19730–19739. [PubMed] [Google Scholar]
  14. Nakato K., Furuno T., Inagaki K., Teshima R., Terao T., Nakanishi M. Cytosolic and intranuclear calcium signals in rat basophilic leukemia cells as revealed by a confocal fluorescence microscope. Eur J Biochem. 1992 Oct 15;209(2):745–749. doi: 10.1111/j.1432-1033.1992.tb17343.x. [DOI] [PubMed] [Google Scholar]
  15. Ryan T. A., Millard P. J., Webb W. W. Imaging [Ca2+]i dynamics during signal transduction. Cell Calcium. 1990 Feb-Mar;11(2-3):145–155. doi: 10.1016/0143-4160(90)90067-5. [DOI] [PubMed] [Google Scholar]
  16. Takemura H., Hughes A. R., Thastrup O., Putney J. W., Jr Activation of calcium entry by the tumor promoter thapsigargin in parotid acinar cells. Evidence that an intracellular calcium pool and not an inositol phosphate regulates calcium fluxes at the plasma membrane. J Biol Chem. 1989 Jul 25;264(21):12266–12271. [PubMed] [Google Scholar]
  17. Teshima R., Ikebuchi H., Terao T., Miyagawa T., Arata Y., Nakanishi M. A single cell observation of staurosporine effect on the Ca2+ signals in rat basophilic leukemia cells. FEBS Lett. 1990 Sep 17;270(1-2):115–118. doi: 10.1016/0014-5793(90)81247-l. [DOI] [PubMed] [Google Scholar]
  18. Thastrup O. Role of Ca2(+)-ATPases in regulation of cellular Ca2+ signalling, as studied with the selective microsomal Ca2(+)-ATPase inhibitor, thapsigargin. Agents Actions. 1990 Jan;29(1-2):8–15. doi: 10.1007/BF01964706. [DOI] [PubMed] [Google Scholar]
  19. Yamada H., Mizuguchi J., Nakanishi M. Antigen receptor-mediated calcium signals in B cells as revealed by confocal fluorescence microscopy. FEBS Lett. 1991 Jun 24;284(2):249–251. doi: 10.1016/0014-5793(91)80696-z. [DOI] [PubMed] [Google Scholar]

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