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. 1994 Nov 15;304(Pt 1):57–60. doi: 10.1042/bj3040057

Thapsigargin-induced nuclear calcium signals in rat basophilic leukaemia cells.

Y Horikoshi 1, T Furuno 1, R Teshima 1, J Sawada 1, M Nakanishi 1
PMCID: PMC1137451  PMID: 7998957

Abstract

By a confocal fluorescence microscope with an argon-ion laser (488 nm) and a He-Cd laser (325 nm) we have studied thapsigargin-induced calcium signals in individual rat basophilic leukaemia (RBL-2H3) cells. In the presence or absence of external calcium ions, thapsigargin-induced calcium signals were transferred to the nucleus as well as to the cytoplasm of RBL-2H3 cells. The calcium signals were generally much stronger in the nucleus than in the cytoplasm. However, some of the RBL-2H3 cells had apparently reduced nuclear calcium signals. They had a basophil-like bilobed (multilobed) nucleus, although most RBL-2H3 cells had a mast-cell-like monolobed nucleus. In the cells with a bilobed nucleus, IgE-receptor-mediated calcium signals were neither transferred to the nucleus nor to the cytoplasm. The results gave a new insight into the understanding of the mechanism of the nuclear calcium signals in RBL-2H3 cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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