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. 1994 Feb 1;297(Pt 3):581–584. doi: 10.1042/bj2970581

Regulation of hepatic level of fatty-acid-binding protein by hormones and clofibric acid in the rat.

S Nakagawa 1, Y Kawashima 1, A Hirose 1, H Kozuka 1
PMCID: PMC1137873  PMID: 8110197

Abstract

Regulation of the hepatic level of fatty-acid-binding protein (FABP) by hormones and p-chlorophenoxyisobutyric acid (clofibric acid) was studied. The hepatic level of FABP, measured as the oleic acid-binding capacity of the cytosolic FABP fraction, was decreased in streptozotocin-diabetic rats. The level of FABP was markedly increased in adrenalectomized rats, and the elevation was prevented by the administration of dexamethasone. Hypothyroidism decreased the level of FABP and hyperthyroidism increased it. A high correlation between the incorporation of [14C]oleic acid in vivo into hepatic triacylglycerol and the level of FABP was found for normal, diabetic and adrenalectomized rats. The level of FABP was increased by administration of clofibric acid to rats in any altered hormonal states, as was microsomal 1-acylglycerophosphocholine (1-acyl-GPC) acyltransferase, a peroxisome-proliferator-responsive parameter. These results suggest that the hepatic level of FABP is under regulation by multiple hormones and that clofibric acid induces FABP and 1-acyl-GPC acyltransferase by a mechanism which may be distinct from that by which hormones regulate the level of FABP.

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Selected References

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  1. BLIGH E. G., DYER W. J. A rapid method of total lipid extraction and purification. Can J Biochem Physiol. 1959 Aug;37(8):911–917. doi: 10.1139/o59-099. [DOI] [PubMed] [Google Scholar]
  2. Bass N. M., Manning J. A., Ockner R. K., Gordon J. I., Seetharam S., Alpers D. H. Regulation of the biosynthesis of two distinct fatty acid-binding proteins in rat liver and intestine. Influences of sex difference and of clofibrate. J Biol Chem. 1985 Feb 10;260(3):1432–1436. [PubMed] [Google Scholar]
  3. Bass N. M. The cellular fatty acid binding proteins: aspects of structure, regulation, and function. Int Rev Cytol. 1988;111:143–184. doi: 10.1016/s0074-7696(08)61733-7. [DOI] [PubMed] [Google Scholar]
  4. Bassuk J. A., Tsichlis P. N., Sorof S. Liver fatty acid binding protein is the mitosis-associated polypeptide target of a carcinogen in rat hepatocytes. Proc Natl Acad Sci U S A. 1987 Nov;84(21):7547–7551. doi: 10.1073/pnas.84.21.7547. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Blackburn G. R., Andrews J. P., Custer R. P., Sorof S. Early events during liver carcinogenesis involving two carcinogen:protein complexes. Cancer Res. 1981 Oct;41(10):4039–4049. [PubMed] [Google Scholar]
  6. Brandes R., Arad R. Liver cytosolic fatty acid-binding proteins. Effect of diabetes and starvation. Biochim Biophys Acta. 1983 Feb 7;750(2):334–339. doi: 10.1016/0005-2760(83)90037-1. [DOI] [PubMed] [Google Scholar]
  7. Brandes R., Kaikaus R. M., Lysenko N., Ockner R. K., Bass N. M. Induction of fatty acid binding protein by peroxisome proliferators in primary hepatocyte cultures and its relationship to the induction of peroxisomal beta-oxidation. Biochim Biophys Acta. 1990 Apr 23;1034(1):53–61. doi: 10.1016/0304-4165(90)90152-m. [DOI] [PubMed] [Google Scholar]
  8. Burrier R. E., Manson C. R., Brecher P. Binding of acyl-CoA to liver fatty acid binding protein: effect on acyl-CoA synthesis. Biochim Biophys Acta. 1987 Jun 23;919(3):221–230. doi: 10.1016/0005-2760(87)90261-x. [DOI] [PubMed] [Google Scholar]
  9. Custer R. P., Sorof S. Target polypeptide of a carcinogen is associated with normal mitosis and carcinogen-induced hyperplasias in adult hepatocytes. Proc Natl Acad Sci U S A. 1984 Nov;81(21):6738–6742. doi: 10.1073/pnas.81.21.6738. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Dutta-Roy A. K., Gopalswamy N., Trulzsch D. V. Prostaglandin E1 binds to Z protein of rat liver. Eur J Biochem. 1987 Feb 2;162(3):615–619. doi: 10.1111/j.1432-1033.1987.tb10683.x. [DOI] [PubMed] [Google Scholar]
  11. Eck M. G., Wynn J. O., Carter W. J., Faas F. H. Fatty acid desaturation in experimental diabetes mellitus. Diabetes. 1979 May;28(5):479–485. doi: 10.2337/diab.28.5.479. [DOI] [PubMed] [Google Scholar]
  12. Glatz J. F., Baerwaldt C. C., Veerkamp J. H., Kempen H. J. Diurnal variation of cytosolic fatty acid-binding protein content and of palmitate oxidation in rat liver and heart. J Biol Chem. 1984 Apr 10;259(7):4295–4300. [PubMed] [Google Scholar]
  13. Gordon J. I., Alpers D. H., Ockner R. K., Strauss A. W. The nucleotide sequence of rat liver fatty acid binding protein mRNA. J Biol Chem. 1983 Mar 10;258(5):3356–3363. [PubMed] [Google Scholar]
  14. Gordon J. I., Elshourbagy N., Lowe J. B., Liao W. S., Alpers D. H., Taylor J. M. Tissue specific expression and developmental regulation of two genes coding for rat fatty acid binding proteins. J Biol Chem. 1985 Feb 25;260(4):1995–1998. [PubMed] [Google Scholar]
  15. Issemann I., Green S. Activation of a member of the steroid hormone receptor superfamily by peroxisome proliferators. Nature. 1990 Oct 18;347(6294):645–650. doi: 10.1038/347645a0. [DOI] [PubMed] [Google Scholar]
  16. Kaikaus R. M., Bass N. M., Ockner R. K. Functions of fatty acid binding proteins. Experientia. 1990 Jun 15;46(6):617–630. doi: 10.1007/BF01939701. [DOI] [PubMed] [Google Scholar]
  17. Kawashima Y., Hirose A., Kozuka H. Selective increase in acylation of 1-acylglycerophosphorylcholine in livers of rats and mice by peroxisome proliferators. Biochim Biophys Acta. 1984 Apr 18;793(2):232–237. doi: 10.1016/0005-2760(84)90325-4. [DOI] [PubMed] [Google Scholar]
  18. Kawashima Y., Horii S., Matsunaga T., Hirose A., Adachi T., Kozuka H. Co-induction by peroxisome proliferators of microsomal 1-acylglycerophosphocholine acyltransferase with peroxisomal beta-oxidation in rat liver. Biochim Biophys Acta. 1989 Sep 25;1005(2):123–129. doi: 10.1016/0005-2760(89)90177-x. [DOI] [PubMed] [Google Scholar]
  19. Kawashima Y., Katoh H., Kozuka H. Differential effects of altered hormonal state on the induction of acyl-CoA hydrolases and peroxisomal beta-oxidation by clofibric acid. Biochim Biophys Acta. 1983 Feb 7;750(2):365–372. doi: 10.1016/0005-2760(83)90041-3. [DOI] [PubMed] [Google Scholar]
  20. Kawashima Y., Kozuka H. Increased activity of stearoyl-CoA desaturation in liver from rat fed clofibric acid. Biochim Biophys Acta. 1982 Dec 13;713(3):622–628. [PubMed] [Google Scholar]
  21. Kawashima Y., Kozuka H. Regulation of palmitoyl-CoA chain elongation and linoleoyl-CoA chain elongation in rat liver microsomes and the differential effects of peroxisome proliferators, insulin and thyroid hormone. Biochim Biophys Acta. 1985 Mar 27;834(1):118–123. doi: 10.1016/0005-2760(85)90183-3. [DOI] [PubMed] [Google Scholar]
  22. Kawashima Y., Musoh K., Kozuka H. Peroxisome proliferators enhance linoleic acid metabolism in rat liver. Increased biosynthesis of omega 6 polyunsaturated fatty acids. J Biol Chem. 1990 Jun 5;265(16):9170–9175. [PubMed] [Google Scholar]
  23. Kawashima Y., Nakagawa S., Kozuka H. Effects of some hypolipidemic drugs and phthalic acid esters on fatty acid binding protein in rat liver. J Pharmacobiodyn. 1982 Oct;5(10):771–779. doi: 10.1248/bpb1978.5.771. [DOI] [PubMed] [Google Scholar]
  24. Kawashima Y., Nakagawa S., Tachibana Y., Kozuka H. Effects of peroxisome proliferators on fatty acid-binding protein in rat liver. Biochim Biophys Acta. 1983 Nov 1;754(1):21–27. doi: 10.1016/0005-2760(83)90077-2. [DOI] [PubMed] [Google Scholar]
  25. Kawashima Y., Tachibana Y., Nakagawa S., Kozuka H. Species difference of liver cytosolic fatty acid-binding protein in rat, mouse and guinea pig. Lipids. 1984 Jul;19(7):481–487. doi: 10.1007/BF02534479. [DOI] [PubMed] [Google Scholar]
  26. Khan S. H., Sorof S. Preferential binding of growth inhibitory prostaglandins by the target protein of a carcinogen. Proc Natl Acad Sci U S A. 1990 Dec;87(23):9401–9405. doi: 10.1073/pnas.87.23.9401. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Kushlan M. C., Gollan J. L., Ma W. L., Ockner R. K. Sex differences in hepatic uptake of long chain fatty acids in single-pass perfused rat liver. J Lipid Res. 1981 Mar;22(3):431–436. [PubMed] [Google Scholar]
  28. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  29. Marcus S. L., Miyata K. S., Zhang B., Subramani S., Rachubinski R. A., Capone J. P. Diverse peroxisome proliferator-activated receptors bind to the peroxisome proliferator-responsive elements of the rat hydratase/dehydrogenase and fatty acyl-CoA oxidase genes but differentially induce expression. Proc Natl Acad Sci U S A. 1993 Jun 15;90(12):5723–5727. doi: 10.1073/pnas.90.12.5723. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Mishkin S., Stein L., Fleischner G., Gatmaitan Z., Arias I. M. Z protein in hepatic uptake and esterification of long-chain fatty acids. Am J Physiol. 1975 Jun;228(6):1634–1640. doi: 10.1152/ajplegacy.1975.228.6.1634. [DOI] [PubMed] [Google Scholar]
  31. Ockner R. K., Lysenko N., Manning J. A., Monroe S. E., Burnett D. A. Sex steroid modulation of fatty acid utilization and fatty acid binding protein concentration in rat liver. J Clin Invest. 1980 May;65(5):1013–1023. doi: 10.1172/JCI109753. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Ockner R. K., Manning J. A., Poppenhausen R. B., Ho W. K. A binding protein for fatty acids in cytosol of intestinal mucosa, liver, myocardium, and other tissues. Science. 1972 Jul 7;177(4043):56–58. doi: 10.1126/science.177.4043.56. [DOI] [PubMed] [Google Scholar]
  33. Ohmi N., Bhargava M., Arias I. M. Binding of 3'-methyl-N,N-dimethyl-4-aminoazobenzene metabolites to rat liver cytosol proteins and ligandin subunits. Cancer Res. 1981 Sep;41(9 Pt 1):3461–3464. [PubMed] [Google Scholar]
  34. Paulussen R. J., Geelen M. J., Beynen A. C., Veerkamp J. H. Immunochemical quantitation of fatty-acid-binding proteins. I. Tissue and intracellular distribution, postnatal development and influence of physiological conditions on rat heart and liver FABP. Biochim Biophys Acta. 1989 Feb 6;1001(2):201–209. doi: 10.1016/0005-2760(89)90149-5. [DOI] [PubMed] [Google Scholar]
  35. Raza H., Pongubala J. R., Sorof S. Specific high affinity binding of lipoxygenase metabolites of arachidonic acid by liver fatty acid binding protein. Biochem Biophys Res Commun. 1989 Jun 15;161(2):448–455. doi: 10.1016/0006-291x(89)92619-3. [DOI] [PubMed] [Google Scholar]
  36. Reddy J. K., Lalwai N. D. Carcinogenesis by hepatic peroxisome proliferators: evaluation of the risk of hypolipidemic drugs and industrial plasticizers to humans. Crit Rev Toxicol. 1983;12(1):1–58. doi: 10.3109/10408448309029317. [DOI] [PubMed] [Google Scholar]
  37. Sheridan M., Wilkinson T. C., Wilton D. C. Studies on fatty acid-binding proteins. Changes in the concentration of hepatic fatty acid-binding protein during development in the rat. Biochem J. 1987 Mar 15;242(3):919–922. doi: 10.1042/bj2420919. [DOI] [PMC free article] [PubMed] [Google Scholar]
  38. Tugwood J. D., Issemann I., Anderson R. G., Bundell K. R., McPheat W. L., Green S. The mouse peroxisome proliferator activated receptor recognizes a response element in the 5' flanking sequence of the rat acyl CoA oxidase gene. EMBO J. 1992 Feb;11(2):433–439. doi: 10.1002/j.1460-2075.1992.tb05072.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  39. Veerkamp J. H., Peeters R. A., Maatman R. G. Structural and functional features of different types of cytoplasmic fatty acid-binding proteins. Biochim Biophys Acta. 1991 Jan 4;1081(1):1–24. doi: 10.1016/0005-2760(91)90244-c. [DOI] [PubMed] [Google Scholar]
  40. Wilkinson T. C., Wilton D. C. Studies on fatty acid-binding proteins. The detection and quantification of the protein from rat liver by using a fluorescent fatty acid analogue. Biochem J. 1986 Sep 1;238(2):419–424. doi: 10.1042/bj2380419. [DOI] [PMC free article] [PubMed] [Google Scholar]
  41. Wilkinson T. C., Wilton D. C. Studies on fatty acid-binding proteins. The diurnal variation shown by rat liver fatty acid-binding protein. Biochem J. 1987 Mar 15;242(3):913–917. doi: 10.1042/bj2420913. [DOI] [PMC free article] [PubMed] [Google Scholar]
  42. Zhang B., Marcus S. L., Sajjadi F. G., Alvares K., Reddy J. K., Subramani S., Rachubinski R. A., Capone J. P. Identification of a peroxisome proliferator-responsive element upstream of the gene encoding rat peroxisomal enoyl-CoA hydratase/3-hydroxyacyl-CoA dehydrogenase. Proc Natl Acad Sci U S A. 1992 Aug 15;89(16):7541–7545. doi: 10.1073/pnas.89.16.7541. [DOI] [PMC free article] [PubMed] [Google Scholar]

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