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. 1994 May 1;299(Pt 3):825–829. doi: 10.1042/bj2990825

A rapid and simple method for the purification of transglutaminase from Streptoverticillium mobaraense.

U Gerber 1, U Jucknischke 1, S Putzien 1, H L Fuchsbauer 1
PMCID: PMC1138095  PMID: 7910736

Abstract

Transglutaminase from Streptoverticillium mobaraense was partially purified by ion-exchange chromatography on a weak acid material and hydrophobic chromatography. The separation with a strong acid ion-exchanger produces homogeneous transglutaminase, in a single step and with high yields, directly from the centrifuged and filtered culture fluid of the micro-organism. The procedure reproduced several times could be also carried out on a larger scale with the optimized parameters of the laboratory isolations. The purified enzyme demonstrated good storage stability.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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