Skip to main content
Biochemical Journal logoLink to Biochemical Journal
. 1989 Jun 1;260(2):593–596. doi: 10.1042/bj2600593

The Ins(1,4,5)P3 binding site of bovine adrenocortical microsomes: function and regulation.

S Palmer 1, M J Wakelam 1
PMCID: PMC1138710  PMID: 2548481

Abstract

Adrenocortical microsomes possess a single population of Ins(1,4,5)P3-specific binding sites [IC50 5.9 +/- 0.9 nM; Palmer, Hughes, Lee & Wakelam (1988) Cell. Signalling 1, 147-156]. Competition studies showed that Ins(1:2-cyclic,4,5)P3 exhibits a 21-fold lower affinity for the site than Ins(1,4,5)P3 (IC50 124 +/- 16 nM). The affinity of the binding sites for Ins(1,4,5)P3 was not influenced by the non-hydrolysable GTP analogues GTP gamma S and Gpp[NH]p or by preincubation of the binding protein with a preparation of partially purified protein kinase C in the presence of ATP and TPA (12-O-tetradecanoylphorbol 13-acetate). These observations are discussed with reference to the identify and function of the Ins(1,4,5)P3 binding site.

Full text

PDF
593

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baukal A. J., Guillemette G., Rubin R., Spät A., Catt K. J. Binding sites for inositol trisphosphate in the bovine adrenal cortex. Biochem Biophys Res Commun. 1985 Dec 17;133(2):532–538. doi: 10.1016/0006-291x(85)90939-8. [DOI] [PubMed] [Google Scholar]
  2. Berridge M. J. Inositol trisphosphate and diacylglycerol as second messengers. Biochem J. 1984 Jun 1;220(2):345–360. doi: 10.1042/bj2200345. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Berridge M. J., Irvine R. F. Inositol trisphosphate, a novel second messenger in cellular signal transduction. Nature. 1984 Nov 22;312(5992):315–321. doi: 10.1038/312315a0. [DOI] [PubMed] [Google Scholar]
  4. Biden T. J., Altin J. G., Karjalainen A., Bygrave F. L. Stimulation of hepatic inositol 1,4,5-trisphosphate kinase activity by Ca2+-dependent and -independent mechanisms. Biochem J. 1988 Dec 15;256(3):697–701. doi: 10.1042/bj2560697. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Biden T. J., Vallar L., Wollheim C. B. Regulation of inositol 1,4,5-trisphosphate metabolism in insulin-secreting RINm5F cells. Biochem J. 1988 Apr 15;251(2):435–440. doi: 10.1042/bj2510435. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Brock T. A., Rittenhouse S. E., Powers C. W., Ekstein L. S., Gimbrone M. A., Jr, Alexander R. W. Phorbol ester and 1-oleoyl-2-acetylglycerol inhibit angiotensin activation of phospholipase C in cultured vascular smooth muscle cells. J Biol Chem. 1985 Nov 15;260(26):14158–14162. [PubMed] [Google Scholar]
  7. Brown K. D., Blakeley D. M., Hamon M. H., Laurie M. S., Corps A. N. Protein kinase C-mediated negative-feedback inhibition of unstimulated and bombesin-stimulated polyphosphoinositide hydrolysis in Swiss-mouse 3T3 cells. Biochem J. 1987 Aug 1;245(3):631–639. doi: 10.1042/bj2450631. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Burgess G. M., Irvine R. F., Berridge M. J., McKinney J. S., Putney J. W., Jr Actions of inositol phosphates on Ca2+ pools in guinea-pig hepatocytes. Biochem J. 1984 Dec 15;224(3):741–746. doi: 10.1042/bj2240741. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Crossley I., Swann K., Chambers E., Whitaker M. Activation of sea urchin eggs by inositol phosphates is independent of external calcium. Biochem J. 1988 May 15;252(1):257–262. doi: 10.1042/bj2520257. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Dawson A. P., Comerford J. G., Fulton D. V. The effect of GTP on inositol 1,4,5-trisphosphate-stimulated Ca2+ efflux from a rat liver microsomal fraction. Is a GTP-dependent protein phosphorylation involved? Biochem J. 1986 Mar 1;234(2):311–315. doi: 10.1042/bj2340311. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Dawson A. P. GTP enhances inositol trisphosphate-stimulated Ca2+ release from rat liver microsomes. FEBS Lett. 1985 Jun 3;185(1):147–150. doi: 10.1016/0014-5793(85)80759-6. [DOI] [PubMed] [Google Scholar]
  12. Guillemette G., Balla T., Baukal A. J., Spät A., Catt K. J. Intracellular receptors for inositol 1,4,5-trisphosphate in angiotensin II target tissues. J Biol Chem. 1987 Jan 25;262(3):1010–1015. [PubMed] [Google Scholar]
  13. Hawkins P. T., Berrie C. P., Morris A. J., Downes C. P. Inositol 1,2-cyclic 4,5-trisphosphate is not a product of muscarinic receptor-stimulated phosphatidylinositol 4,5-bisphosphate hydrolysis in rat parotid glands. Biochem J. 1987 Apr 1;243(1):211–218. doi: 10.1042/bj2430211. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Henne V., Piiper A., Söling H. D. Inositol 1,4,5-trisphosphate and 5'-GTP induce calcium release from different intracellular pools. FEBS Lett. 1987 Jun 22;218(1):153–158. doi: 10.1016/0014-5793(87)81037-2. [DOI] [PubMed] [Google Scholar]
  15. Henne V., Söling H. D. Guanosine 5'-triphosphate releases calcium from rat liver and guinea pig parotid gland endoplasmic reticulum independently of inositol 1,4,5-trisphosphate. FEBS Lett. 1986 Jul 7;202(2):267–273. doi: 10.1016/0014-5793(86)80699-8. [DOI] [PubMed] [Google Scholar]
  16. Imboden J. B., Pattison G. Regulation of inositol 1,4,5-trisphosphate kinase activity after stimulation of human T cell antigen receptor. J Clin Invest. 1987 May;79(5):1538–1541. doi: 10.1172/JCI112986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Irvine R. F., Letcher A. J., Lander D. J., Berridge M. J. Specificity of inositol phosphate-stimulated Ca2+ mobilization from Swiss-mouse 3T3 cells. Biochem J. 1986 Nov 15;240(1):301–304. doi: 10.1042/bj2400301. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Irvine R. F., Moor R. M. Micro-injection of inositol 1,3,4,5-tetrakisphosphate activates sea urchin eggs by a mechanism dependent on external Ca2+. Biochem J. 1986 Dec 15;240(3):917–920. doi: 10.1042/bj2400917. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Meyer T., Holowka D., Stryer L. Highly cooperative opening of calcium channels by inositol 1,4,5-trisphosphate. Science. 1988 Apr 29;240(4852):653–656. doi: 10.1126/science.2452482. [DOI] [PubMed] [Google Scholar]
  20. Misbahuddin M., Isosaki M., Houchi H., Oka M. Muscarinic receptor-mediated increase in cytoplasmic free Ca2+ in isolated bovine adrenal medullary cells. Effects of TMB-8 and phorbol ester TPA. FEBS Lett. 1985 Oct 7;190(1):25–28. doi: 10.1016/0014-5793(85)80419-1. [DOI] [PubMed] [Google Scholar]
  21. Niedel J. E., Kuhn L. J., Vandenbark G. R. Phorbol diester receptor copurifies with protein kinase C. Proc Natl Acad Sci U S A. 1983 Jan;80(1):36–40. doi: 10.1073/pnas.80.1.36. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Orellana S. A., Solski P. A., Brown J. H. Phorbol ester inhibits phosphoinositide hydrolysis and calcium mobilization in cultured astrocytoma cells. J Biol Chem. 1985 May 10;260(9):5236–5239. [PubMed] [Google Scholar]
  23. Palmer S., Hughes K. T., Lee D. Y., Wakelam M. J. Development of a novel, Ins(1,4,5)P3-specific binding assay. Its use to determine the intracellular concentration of Ins(1,4,5)P3 in unstimulated and vasopressin-stimulated rat hepatocytes. Cell Signal. 1989;1(2):147–156. doi: 10.1016/0898-6568(89)90004-1. [DOI] [PubMed] [Google Scholar]
  24. Shears S. B. Metabolism of the inositol phosphates produced upon receptor activation. Biochem J. 1989 Jun 1;260(2):313–324. doi: 10.1042/bj2600313. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Supattapone S., Danoff S. K., Theibert A., Joseph S. K., Steiner J., Snyder S. H. Cyclic AMP-dependent phosphorylation of a brain inositol trisphosphate receptor decreases its release of calcium. Proc Natl Acad Sci U S A. 1988 Nov;85(22):8747–8750. doi: 10.1073/pnas.85.22.8747. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Supattapone S., Worley P. F., Baraban J. M., Snyder S. H. Solubilization, purification, and characterization of an inositol trisphosphate receptor. J Biol Chem. 1988 Jan 25;263(3):1530–1534. [PubMed] [Google Scholar]
  27. Ueda T., Chueh S. H., Noel M. W., Gill D. L. Influence of inositol 1,4,5-trisphosphate and guanine nucleotides on intracellular calcium release within the N1E-115 neuronal cell line. J Biol Chem. 1986 Mar 5;261(7):3184–3192. [PubMed] [Google Scholar]
  28. Volpe P., Krause K. H., Hashimoto S., Zorzato F., Pozzan T., Meldolesi J., Lew D. P. "Calciosome," a cytoplasmic organelle: the inositol 1,4,5-trisphosphate-sensitive Ca2+ store of nonmuscle cells? Proc Natl Acad Sci U S A. 1988 Feb;85(4):1091–1095. doi: 10.1073/pnas.85.4.1091. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Willcocks A. L., Cooke A. M., Potter B. V., Nahorski S. R. Stereospecific recognition sites for [3H]inositol(1,4,5)-triphosphate in particulate preparations of rat cerebellum. Biochem Biophys Res Commun. 1987 Aug 14;146(3):1071–1078. doi: 10.1016/0006-291x(87)90756-x. [DOI] [PubMed] [Google Scholar]
  30. Willcocks A. L., Strupish J., Irvine R. F., Nahorski S. R. Inositol 1:2-cyclic,4,5-trisphosphate is only a weak agonist at inositol 1,4,5-trisphosphate receptors. Biochem J. 1989 Jan 1;257(1):297–300. doi: 10.1042/bj2570297. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Wilson D. B., Connolly T. M., Bross T. E., Majerus P. W., Sherman W. R., Tyler A. N., Rubin L. J., Brown J. E. Isolation and characterization of the inositol cyclic phosphate products of polyphosphoinositide cleavage by phospholipase C. Physiological effects in permeabilized platelets and Limulus photoreceptor cells. J Biol Chem. 1985 Nov 5;260(25):13496–13501. [PubMed] [Google Scholar]
  32. Wong N. S., Barker C. J., Shears S. B., Kirk C. J., Michell R. H. Inositol 1:2(cyclic),4,5-trisphosphate is not a major product of inositol phospholipid metabolism in vasopressin-stimulated WRK1 cells. Biochem J. 1988 May 15;252(1):1–5. doi: 10.1042/bj2520001. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES