Skip to main content
PMC home page
Journal of Clinical Pathology logoLink to Journal of Clinical Pathology
. 1987 Jun;40(6):593–600. doi: 10.1136/jcp.40.6.593

Colonic pericrypt sheath cells: characterisation of cell type with new monoclonal antibody.

P I Richman, R Tilly, J R Jass, W F Bodmer
PMCID: PMC1141045  PMID: 3301906

Abstract

A new monoclonal antibody, PR 2D3, was raised against a crude homogenate of normal colorectal mucosa and found to react with the cells in the pericrypt sheath. It also reacted with smooth muscle throughout the body and, in specific sites, with those mesenchymal cells known as myofibroblasts. It did not react with cardiac or skeletal muscle, nor with fibroblasts. PR 2D3 is an IgG1 antibody and identifies a membrane component of about 140 K molecular weight. The pericrypt cells have been described as fibroblasts, but in view of the specificity of PR 2D3 for smooth muscle, and its selective staining of the colonic pericrypt cells, this cell type was re-examined for other smooth muscle properties. Ultrastructurally, the cells had many characteristics in common with smooth muscle and were identical with the myofibroblasts of the umbilical cord. On immunocytochemical examination they were found to contain desmin, myosin, and filamin. The confirmation that the pericrypt cells are myofibroblasts suggest that they have both contractile and secretory roles.

Full text

PDF
595

Images in this article

597Fig 6
on p.597
595Fig 1
on p.595
595Fig 2
on p.595
595Fig 3
on p.595
596Fig 4
on p.596
596Fig 5
on p.596
598Fig 7
on p.598
598Fig 8
on p.598

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Balázs M., Kovács A. The 'transitional' mucosa adjacent to large bowel carcinoma--electron microscopic features and myofibroblast reaction. Histopathology. 1982 Sep;6(5):617–629. doi: 10.1111/j.1365-2559.1982.tb02755.x. [DOI] [PubMed] [Google Scholar]
  2. DEANE H. W. SOME ELECTRON MICROSCOPIC OBSERVATIONS ON THE LAMINA PROPRIA OF THE GUT, WITH COMMENTS ON THE CLOSE ASSOCIATION OF MACROPHAGES, PLASMA CELLS, AND EOSINOPHILS. Anat Rec. 1964 Jul;149:453–473. doi: 10.1002/ar.1091490315. [DOI] [PubMed] [Google Scholar]
  3. Donnellan W. L. The structure of the colonic mucosa. The epithelium and subepithelial reticulohistiocytic complex. Gastroenterology. 1965 Nov;49(5):496–514. [PubMed] [Google Scholar]
  4. Hwang W. S., Kelly J. K., Shaffer E. A., Hershfield N. B. Collagenous colitis: a disease of pericryptal fibroblast sheath? J Pathol. 1986 May;149(1):33–40. doi: 10.1002/path.1711490108. [DOI] [PubMed] [Google Scholar]
  5. Kaye G. I., Lane N., Pascal R. R. Colonic pericryptal fibroblast sheath: replication, migration, and cytodifferentiation of a mesenchymal cell system in adult tissue. II. Fine structural aspects of normal rabbit and human colon. Gastroenterology. 1968 May;54(5):852–865. [PubMed] [Google Scholar]
  6. Lee E. Y., Parry G., Bissell M. J. Modulation of secreted proteins of mouse mammary epithelial cells by the collagenous substrata. J Cell Biol. 1984 Jan;98(1):146–155. doi: 10.1083/jcb.98.1.146. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Majno G., Gabbiani G., Hirschel B. J., Ryan G. B., Statkov P. R. Contraction of granulation tissue in vitro: similarity to smooth muscle. Science. 1971 Aug 6;173(3996):548–550. doi: 10.1126/science.173.3996.548. [DOI] [PubMed] [Google Scholar]
  8. Marsh M. N., Trier J. S. Morphology and cell proliferation of subepithelial fibroblasts in adult mouse jejunum. I. Structural features. Gastroenterology. 1974 Oct;67(4):622–635. [PubMed] [Google Scholar]
  9. Maskens A. P., Rahier J. R., Meersseman F. P., Dujardin-Loits R. M., Haot J. G. Cell proliferation of pericryptal fibroblasts in the rat colon mucosa. Gut. 1979 Sep;20(9):775–779. doi: 10.1136/gut.20.9.775. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Mathan M., Hermos J. A., Trier J. S. Structural features of the epithelio-mesenchymal interface of rat duodenal mucosa during development. J Cell Biol. 1972 Mar;52(3):577–588. doi: 10.1083/jcb.52.3.577. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Neal J. V., Potten C. S. Description and basic cell kinetics of the murine pericryptal fibroblast sheath. Gut. 1981 Jan;22(1):19–24. doi: 10.1136/gut.22.1.19. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Parker F. G., Barnes E. N., Kaye G. I. The pericryptal fibroblast sheath. IV. Replication, migration, and differentiation of the subepithelial fibroblasts of the crypt and villus of the rabbit jejunum. Gastroenterology. 1974 Oct;67(4):607–621. [PubMed] [Google Scholar]
  13. Parry E. W. Some electron microscope observations on the mesenchymal structures of full-term umbilical cord. J Anat. 1970 Nov;107(Pt 3):505–518. [PMC free article] [PubMed] [Google Scholar]
  14. Pascal R. R., Kaye G. I., Lane N. Colonic pericryptal fibroblast sheath: replication, migration, and cytodifferentiation of a mesenchymal cell system in adult tissue. I. Autoradiographic studies of normal rabbit colon. Gastroenterology. 1968 May;54(5):835–851. [PubMed] [Google Scholar]
  15. Polak J. A., Bloom S. R. Localization of regulatory peptides in the gut. Br Med Bull. 1982 Sep;38(3):303–307. doi: 10.1093/oxfordjournals.bmb.a071777. [DOI] [PubMed] [Google Scholar]
  16. Small J. V., Fürst D. O., De Mey J. Localization of filamin in smooth muscle. J Cell Biol. 1986 Jan;102(1):210–220. doi: 10.1083/jcb.102.1.210. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Towbin H., Staehelin T., Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350–4354. doi: 10.1073/pnas.76.9.4350. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Wissler R. W. The arterial medial cell, smooth muscle, or multifunctional mesenchyme? Circulation. 1967 Jul;36(1):1–4. doi: 10.1161/01.cir.36.1.1. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Clinical Pathology are provided here courtesy of BMJ Publishing Group

RESOURCES