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. 1988 Sep;41(9):983–988. doi: 10.1136/jcp.41.9.983

Monoclonal antibodies show Listeria monocytogenes in necropsy tissue samples.

J McLauchlin 1, A Black 1, H T Green 1, J Q Nash 1, A G Taylor 1
PMCID: PMC1141657  PMID: 3142937

Abstract

Stable mouse monoclonal hybridoma cell lines secreting antibodies against Listeria monocytogenes were produced. Antibodies from two of these cell lines (designated CL2 and CL17) have been partially characterised. The specificities of these antibodies were assessed using indirect immunofluorescence antibody tests and L monocytogenes (166 strains) grown in vitro, other species of Listeria (21 strains), and bacteria from 14 other genera (87 strains). The antibodies were found to be specific for Listeria, and when used in combination, reacted with almost all strains of L monocytogenes. A simple and rapid direct immunofluorescence technique was developed, and the presence of L monocytogenes was shown in necropsy tissue from three patients where listeriosis had been confirmed by isolation of the bacterium. Bacteria were also confirmed using one of these antibodies in necropsy tissue from one further patient in whom listeriosis was suspected, but not confirmed by the cultivation of L monocytogenes.

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Selected References

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  1. Aalund O., Osebold J. W., Murphy F. A., Di Capua R. A. Antibody heterogeneity in experimental listeriosis. J Immunol. 1966 Jul;97(1):150–157. [PubMed] [Google Scholar]
  2. Antonissen A. C., van Kessel K. P., van Dijk H., Willers J. M. Development of a simple passive haemagglutination-inhibition assay for Listeria monocytogenes lipoteichoic acid. J Immunol Methods. 1981;44(3):351–357. doi: 10.1016/0022-1759(81)90053-3. [DOI] [PubMed] [Google Scholar]
  3. Busch L. A. Human listeriosis in the United States, 1967-1969. J Infect Dis. 1971 Mar;123(3):328–332. doi: 10.1093/infdis/123.3.328. [DOI] [PubMed] [Google Scholar]
  4. Centers for Disease Control (CDC) Listeriosis outbreak associated with Mexican-style cheese--California. MMWR Morb Mortal Wkly Rep. 1985 Jun 21;34(24):357–359. [PubMed] [Google Scholar]
  5. Dee R. R., Lorber B. Brain abscess due to Listeria monocytogenes: case report and literature review. Rev Infect Dis. 1986 Nov-Dec;8(6):968–977. doi: 10.1093/clinids/8.6.968. [DOI] [PubMed] [Google Scholar]
  6. EVELAND W. C. DEMONSTRATION OF LISTERIA MONOCYTOGENES IN DIRECT EXAMINATION OF SPINAL FLUID BY FLUORESCENT-ANTIBODY TECHNIQUE. J Bacteriol. 1963 Jun;85:1448–1450. doi: 10.1128/jb.85.6.1448-1450.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Fleming D. W., Cochi S. L., MacDonald K. L., Brondum J., Hayes P. S., Plikaytis B. D., Holmes M. B., Audurier A., Broome C. V., Reingold A. L. Pasteurized milk as a vehicle of infection in an outbreak of listeriosis. N Engl J Med. 1985 Feb 14;312(7):404–407. doi: 10.1056/NEJM198502143120704. [DOI] [PubMed] [Google Scholar]
  8. Gray M. L., Killinger A. H. Listeria monocytogenes and listeric infections. Bacteriol Rev. 1966 Jun;30(2):309–382. doi: 10.1128/br.30.2.309-382.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Green H. T., Macaulay M. B. Hospital outbreak of Listeria monocytogenes septicaemia: a problem of cross infection? Lancet. 1978 Nov 11;2(8098):1039–1040. doi: 10.1016/s0140-6736(78)92352-8. [DOI] [PubMed] [Google Scholar]
  10. Hether N. W., Jackson L. L. Lipoteichoic acid from Listeria monocytogenes. J Bacteriol. 1983 Nov;156(2):809–817. doi: 10.1128/jb.156.2.809-817.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Hone R., Marshall P., Cran H. Listeria monocytogenes meningitis in a healthy adult. J Ir Med Assoc. 1972 Feb 19;65(4):81–82. [PubMed] [Google Scholar]
  12. Hopfer R. L., Pinzon R., Wenglar M., Rolston K. V. Enzyme release of antigen from Streptococcus faecalis and Listeria monocytogenes cross-reactive with Lancefield group G typing reagents. J Clin Microbiol. 1985 Oct;22(4):677–679. doi: 10.1128/jcm.22.4.677-679.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Kennard C., Howard A. J., Scholtz C., Swash M. Infection of the brainstem by Listeria monocytogenes. J Neurol Neurosurg Psychiatry. 1979 Oct;42(10):931–933. doi: 10.1136/jnnp.42.10.931. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Khan M. A., Seaman A., Woodbine M. Immunofluorescent identification of Listeria monocytogenes. Zentralbl Bakteriol Orig A. 1977 Sep;239(1):62–69. [PubMed] [Google Scholar]
  15. Khong T. Y., Frappell J. M., Steel H. M., Stewart C. M., Burke M. Perinatal listeriosis. A report of six cases. Br J Obstet Gynaecol. 1986 Oct;93(10):1083–1087. doi: 10.1111/j.1471-0528.1986.tb07835.x. [DOI] [PubMed] [Google Scholar]
  16. Klatt E. C., Pavlova Z., Teberg A. J., Yonekura M. L. Epidemic perinatal listeriosis at autopsy. Hum Pathol. 1986 Dec;17(12):1278–1281. doi: 10.1016/s0046-8177(86)80572-x. [DOI] [PubMed] [Google Scholar]
  17. Larsen S. A., Jones W. L. Evaluation and standardization of an agglutination test for human listeriosis. Appl Microbiol. 1972 Jul;24(1):101–107. doi: 10.1128/am.24.1.101-107.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. McLauchlin J., Audurier A., Taylor A. G. The evaluation of a phage-typing system for Listeria monocytogenes for use in epidemiological studies. J Med Microbiol. 1986 Dec;22(4):357–365. doi: 10.1099/00222615-22-4-357. [DOI] [PubMed] [Google Scholar]
  19. McLauchlin J. Listeria monocytogenes, recent advances in the taxonomy and epidemiology of listeriosis in humans. J Appl Bacteriol. 1987 Jul;63(1):1–11. doi: 10.1111/j.1365-2672.1987.tb02411.x. [DOI] [PubMed] [Google Scholar]
  20. Minden P., McClatchy J. K., Farr R. S. Shared antigens between heterologous bacterial species. Infect Immun. 1972 Oct;6(4):574–582. doi: 10.1128/iai.6.4.574-582.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Nieman R. E., Lorber B. Listeriosis in adults: a changing pattern. Report of eight cases and review of the literature, 1968-1978. Rev Infect Dis. 1980 Mar-Apr;2(2):207–227. doi: 10.1093/clinids/2.2.207. [DOI] [PubMed] [Google Scholar]
  22. Pease P. E., Nicholls L., Stuart M. R. Evidence that precipitin cross-reactions between Listeria, Erysipelothrix and Bacillus licheniformis are not due to the Rantz antigen. J Gen Microbiol. 1972 Dec;73(3):567–569. doi: 10.1099/00221287-73-3-567. [DOI] [PubMed] [Google Scholar]
  23. Pollock S. S., Pollock T. M., Harrison M. J. Infection of the central nervous system by Listeria monocytogenes: a review of 54 adult and juvenile cases. Q J Med. 1984 Summer;53(211):331–340. [PubMed] [Google Scholar]
  24. Relier J. P. Perinatal and neonatal infections: listeriosis. J Antimicrob Chemother. 1979 May;5 (Suppl A):51–57. doi: 10.1093/jac/5.supplement_a.51. [DOI] [PubMed] [Google Scholar]
  25. Robertson M. H., Mussalli N. G., Aizad T. A., Okaro J. M., Banwell G. S. Two cases of perinatal listeriosis. Arch Dis Child. 1979 Jul;54(7):549–551. doi: 10.1136/adc.54.7.549. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Rocourt J., Hof H., Schrettenbrunner A., Malinverni R., Bille J. Méningite purulente aiguë à Listeria seeligeri chez un adulte immunocompétent. Schweiz Med Wochenschr. 1986 Feb 22;116(8):248–251. [PubMed] [Google Scholar]
  27. Rocourt J., Schrettenbrunner A., Seeliger H. P. Différenciation biochimique des groupes génomiques de Listeria monocytogenes (sensu lato). Ann Microbiol (Paris) 1983 Jan-Feb;134A(1):65–71. [PubMed] [Google Scholar]
  28. Rocourt J., Seeliger H. P. Distribution des espèces du genre Listeria. Zentralbl Bakteriol Mikrobiol Hyg A. 1985 May;259(3):317–330. [PubMed] [Google Scholar]
  29. Russell W. C., Patel G., Precious B., Sharp I., Gardner P. S. Monoclonal antibodies against adenovirus type 5: preparation and preliminary characterization. J Gen Virol. 1981 Oct;56(Pt 2):393–408. doi: 10.1099/0022-1317-56-2-393. [DOI] [PubMed] [Google Scholar]
  30. SEELIGER H. P., SULZBACHER F. Antigenic relationships between Listeria monocytogenes and Staphylococcus aureus. Can J Microbiol. 1956 May;2(3):220–231. doi: 10.1139/m56-027. [DOI] [PubMed] [Google Scholar]
  31. SMITH C. W., MARSHALL J. D., Jr, EVELAND W. C. Identification of Listeria monocytogenes by the fluorescent antibody technic. Proc Soc Exp Biol Med. 1960 Apr;103:842–845. doi: 10.3181/00379727-103-25691. [DOI] [PubMed] [Google Scholar]
  32. Samuel D., Patt R. J., Abuknesha R. A. A sensitive method of detecting proteins on dot and Western blots using a monoclonal antibody to FITC. J Immunol Methods. 1988 Mar 16;107(2):217–224. doi: 10.1016/0022-1759(88)90221-9. [DOI] [PubMed] [Google Scholar]
  33. Schlech W. F., 3rd, Lavigne P. M., Bortolussi R. A., Allen A. C., Haldane E. V., Wort A. J., Hightower A. W., Johnson S. E., King S. H., Nicholls E. S. Epidemic listeriosis--evidence for transmission by food. N Engl J Med. 1983 Jan 27;308(4):203–206. doi: 10.1056/NEJM198301273080407. [DOI] [PubMed] [Google Scholar]
  34. Trautmann M., Wagner J., Chahin M., Weinke T. Listeria meningitis: report of ten recent cases and review of current therapeutic recommendations. J Infect. 1985 Mar;10(2):107–114. doi: 10.1016/s0163-4453(85)91501-4. [DOI] [PubMed] [Google Scholar]
  35. WELSHIMER H. J. Staphylococcal antibody production in response to injections with Listeria monocytogenes. J Bacteriol. 1960 Mar;79:456–457. doi: 10.1128/jb.79.3.456-457.1960. [DOI] [PMC free article] [PubMed] [Google Scholar]
  36. Watson B. B., Eveland W. C. The application of the phage-fluorescent antiphage staining system in the specific identification of Listeria monocytogenes. I. Species specificity and immunofluorescent sensitivity of Listeria monocytogenes phage observed in smear preparations. J Infect Dis. 1965 Oct;115(4):363–369. doi: 10.1093/infdis/115.4.363. [DOI] [PubMed] [Google Scholar]

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