Skip to main content
Journal of Clinical Pathology logoLink to Journal of Clinical Pathology
. 1989 Feb;42(2):206–209. doi: 10.1136/jcp.42.2.206

Biotin-labelled antigen screening test for toxoplasma IgM antibody.

A W Joss 1, L J Skinner 1, I L Moir 1, J M Chatterton 1, H Williams 1, D O Ho-Yen 1
PMCID: PMC1141829  PMID: 2921363

Abstract

A method for the simple preparation of biotin-labelled toxoplasma antigen was used with avidin peroxidase in an IgM-capture enzyme linked immunosorbent assay (BAM-ELISA). Although the overall predictive value of a positive result was only 38%, its low cost and 100% sensitivity makes it a very suitable screening test. Positive results can be confirmed by an alternative assay, thus providing a more economical and effective diagnostic service than either screening all sera by a commercial test or selecting sera for IgM testing.

Full text

PDF
206

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adler-Storthz K., Kendall C., Kennedy R. C., Henkel R. D., Dreesman G. R. Biotin-avidin-amplified enzyme immunoassay for detection of herpes simplex virus antigen in clinical specimens. J Clin Microbiol. 1983 Dec;18(6):1329–1334. doi: 10.1128/jcm.18.6.1329-1334.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Balfour A. H., Harford J. P. Detection of specific IgG and IgM antibodies to Toxoplasma gondii with a commercially available enzyme immunoassay kit system. J Clin Pathol. 1985 Jun;38(6):679–689. doi: 10.1136/jcp.38.6.679. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Buckley C. H., Butler E. B., Fox H. Cervical intraepithelial neoplasia. J Clin Pathol. 1982 Jan;35(1):1–13. doi: 10.1136/jcp.35.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Joss A. W., Skinner L. J., Chatterton J. M., Chisholm S. M., Williams H. D., Ho-Yen D. O. Simultaneous serological screening for congenital cytomegalovirus and toxoplasma infection. Public Health. 1988 Sep;102(5):409–417. doi: 10.1016/s0033-3506(88)80078-7. [DOI] [PubMed] [Google Scholar]
  5. Naot Y., Barnett E. V., Remington J. S. Method for avoiding false-positive results occurring in immunoglobulin M enzyme-linked immunosorbent assays due to presence of both rheumatoid factor and antinuclear antibodies. J Clin Microbiol. 1981 Jul;14(1):73–78. doi: 10.1128/jcm.14.1.73-78.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Payne R. A., Joynson D. H., Balfour A. H., Harford J. P., Fleck D. G., Mythen M., Saunders R. J. Public Health Laboratory Service enzyme linked immunosorbent assay for detecting Toxoplasma specific IgM antibody. J Clin Pathol. 1987 Mar;40(3):276–281. doi: 10.1136/jcp.40.3.276. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Sabin A. B., Feldman H. A. Dyes as Microchemical Indicators of a New Immunity Phenomenon Affecting a Protozoon Parasite (Toxoplasma). Science. 1948 Dec 10;108(2815):660–663. doi: 10.1126/science.108.2815.660. [DOI] [PubMed] [Google Scholar]
  8. Wielaard F., van Gruijthuijsen H., Duermeyer W., Joss A. W., Skinner L., Williams H., van Elven E. H. Diagnosis of acute toxoplasmosis by an enzyme immunoassay for specific immunoglobulin m antibodies. J Clin Microbiol. 1983 Jun;17(6):981–987. doi: 10.1128/jcm.17.6.981-987.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Williams K. A., Scott J. M., Macfarlane D. E., Williamson J. M., Elias-Jones T. F., Williams H. Congenital toxoplasmosis: a prospective survey in the West of Scotland. J Infect. 1981 Sep;3(3):219–229. doi: 10.1016/s0163-4453(81)90773-8. [DOI] [PubMed] [Google Scholar]
  10. van Loon A. M., van der Logt J. T., Heessen F. W., van der Veen J. Enzyme-linked immunosorbent assay that uses labeled antigen for detection of immunoglobulin M and A antibodies in toxoplasmosis: comparison with indirect immunofluorescence and double-sandwich enzyme-linked immunosorbent assay. J Clin Microbiol. 1983 Jun;17(6):997–1004. doi: 10.1128/jcm.17.6.997-1004.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Clinical Pathology are provided here courtesy of BMJ Publishing Group

RESOURCES