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Journal of Clinical Pathology logoLink to Journal of Clinical Pathology
. 1989 Jul;42(7):719–722. doi: 10.1136/jcp.42.7.719

Comparison of western blot analysis and immunocytochemical detection of P-glycoprotein in multidrug resistant cells.

M L Friedlander 1, D R Bell 1, J Leary 1, R A Davey 1
PMCID: PMC1142021  PMID: 2569484

Abstract

A sensitive immunocytochemical technique was developed to detect a 170,000 dalton cell membrane glycoprotein (P-gp) in cell lines resistant to vincristine and vinblastine with varying degrees of resistance. P-gp was shown very clearly using the C219 monoclonal antibody and immunocytochemical detection with either antialkaline phosphate or peroxidase-antiperoxidase with silver gold intensification. There was good correlation between the results obtained with immunocytochemical detection of P-gp in single cells and Western blot analysis. The technique is easily performed and can detect P-gp in relatively small numbers of cells that Western blot analysis could miss and is suitable for clinical application.

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Selected References

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