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. 1984 Aug 1;221(3):665–673. doi: 10.1042/bj2210665

Extraction, purification and identification of cytidine 3',5'-cyclic monophosphate from rat tissues.

R P Newton, S G Salih, B J Salvage, E E Kingston
PMCID: PMC1144096  PMID: 6089744

Abstract

The large-scale extraction and purification to homogeneity of cyclic CMP and its unequivocal identification are described. Rat liver, kidney, heart, spleen and lung tissues were subjected to a sequential purification procedure involving freeze-clamping, perchlorate extraction, alumina and boronate column chromatography, polyacrylamide-gel column electrophoresis and high-voltage paper electrophoresis. The purified sample co-chromatographed with authentic cyclic CMP on t.l.c. and high-pressure liquid chromatography and was positive in a cyclic CMP radio-immunoassay. The u.v., i.r. and p.m.r. spectra were each essentially identical with those of authentic cyclic CMP. Fast-atom bombardment of authentic cyclic CMP yielded a mass spectrum containing a molecular protonated ion: mass-ion-kinetic-energy scanning of this ion produced a spectrum unique to 3',5'-cyclic CMP. The extracted nucleotide produced an identical mass-ion-kinetic-energy spectrum.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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