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. 1984 Nov 1;223(3):855–859. doi: 10.1042/bj2230855

Activation of phospholipase C and prostaglandin synthesis by [arginine]vasopressin in cultures.

J Pfeilschifter, A Kurtz, C Bauer
PMCID: PMC1144372  PMID: 6439189

Abstract

[Arginine]vasopressin (AVP) stimulates maximal prostaglandin E2 production in cultured rat renal mesangial cells within 2 min. As early as 10s after addition of AVP (10(-6)M) a significant loss of radioactivity from phosphatidylinositol 4,5-bisphosphate but not from phosphatidylinositol 4-phosphate and phosphatidylinositol was observed in cells prelabelled with 32Pi. Cells labelled with [14C]arachidonic acid showed an increase of label in 1,2-diacylglycerol after 15 s and in phosphatidic acid after 30 s upon stimulation with AVP. Pretreatment of the cells with indomethacin (10(-5)M) did not abolish the effect of AVP on the increased labelling of phosphatidic acid.

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Selected References

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