Skip to main content
PMC home page
Biochemical Journal logoLink to Biochemical Journal
. 1985 Jan 1;225(1):127–133. doi: 10.1042/bj2250127

Identification of intestinal cells responsive to calcitriol (1,25-dihydroxycholecalciferol).

M W Smith, M E Bruns, E D Lawson
PMCID: PMC1144560  PMID: 3838467

Abstract

The location of intestinal cells taken from the base of the crypt to the tip of the villus responsive to calcitriol (1,25-dihydroxycholecalciferol) and the distribution of [3H]calcitriol within the intestinal epithelium has been determined in vitamin D-deficient rats. The calcitriol responses examined were CaBP (Ca2+-binding protein) levels as measured by immunodiffusion and alkaline phosphatase levels as determined cytochemically. Calcitriol had no effect on villus structure or on enterocyte kinetics. This made it possible to compare levels of CaBP and alkaline phosphatase activity in enterocytes at different ages in rats at known times after hormone injection. Cells from both the crypt and villus synthesized CaBP in response to calcitriol. Alkaline phosphatase activity was not detectable in crypt cells, although a pool of precursor was produced in these cells in response to calcitriol. Enzyme activity was increased in all villus cells in response to calcitriol, but the quantitative description of this effect was very different from that found for calcitriol effects on CaBP synthesis. Calcitriol injected into vitamin D-deficient rats was detected, within 2h, in all cells of the crypt and villus. Most of the binding was to sites having a high affinity for the injected hormone.

Full text

PDF
131

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Armbrecht H. J., Zenser T. V., Davis B. B. Effect of vitamin D metabolites on intestinal calcium absorption and calcium-binding protein in young and adult rats. Endocrinology. 1980 Feb;106(2):469–475. doi: 10.1210/endo-106-2-469. [DOI] [PubMed] [Google Scholar]
  2. Arnold B. M., Kovacs K., Murray T. M. Cellular localization of intestinal calcium-binding protein in pig duodenum. Digestion. 1976;14(1):77–84. doi: 10.1159/000197801. [DOI] [PubMed] [Google Scholar]
  3. Bruns M. E., Fausto A., Avioli L. V. Placental calcium binding protein in rats. Apparent identity with vitamin D-dependent calcium binding protein from rat intestine. J Biol Chem. 1978 May 10;253(9):3186–3190. [PubMed] [Google Scholar]
  4. Gutschmidt S., Lange U., Riecken E. O. Kinetic characterization of unspecific alkaline phosphatase at different villus sites of rat jejunum. A quantitative histochemical study. Histochemistry. 1980;69(2):189–202. doi: 10.1007/BF00533136. [DOI] [PubMed] [Google Scholar]
  5. Jande S. S., Tolnai S., Lawson D. E. Immunohistochemical localization of vitamin D-dependent calcium-binding protein in duodenum, kidney, uterus and cerebellum of chickens. Histochemistry. 1981;71(1):99–116. doi: 10.1007/BF00592574. [DOI] [PubMed] [Google Scholar]
  6. King I. S., Paterson J. Y., Peacock M. A., Smith M. W., Syme G. Effect of diet upon enterocyte differentiation in the rat jejunum. J Physiol. 1983 Nov;344:465–481. doi: 10.1113/jphysiol.1983.sp014952. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Markwell M. A., Haas S. M., Bieber L. L., Tolbert N. E. A modification of the Lowry procedure to simplify protein determination in membrane and lipoprotein samples. Anal Biochem. 1978 Jun 15;87(1):206–210. doi: 10.1016/0003-2697(78)90586-9. [DOI] [PubMed] [Google Scholar]
  8. Morrissey R. L., Empson R. N., Jr, Zolock D. T., Bikle D. D., Bucci T. J. Intestinal response to 1alpha,25-dihydroxycholecalciferol. II. A timed study of the intracellular localization of calcium binding protein. Biochim Biophys Acta. 1978 Jan 3;538(1):34–41. doi: 10.1016/0304-4165(78)90249-0. [DOI] [PubMed] [Google Scholar]
  9. Sampson H. W., Krawitt E. L. A morphometric investigation of the duodenal mucosa of normal, vitamin D-deficient, and vitamin D-replete rats. Calcif Tissue Res. 1976 Dec 2;21(3):213–218. doi: 10.1007/BF02547398. [DOI] [PubMed] [Google Scholar]
  10. Shinki T., Takahashi N., Kawate N., Suda T. The possible role of calcium-binding protein induced by 1 alpha,25-dihydroxyvitamin D3 in the intestinal calcium transport mechanism. Endocrinology. 1982 Nov;111(5):1546–1551. doi: 10.1210/endo-111-5-1546. [DOI] [PubMed] [Google Scholar]
  11. Smith M. W., Moor R. M. Protein synthesis and secretion by hamster, rat and mouse jejunum. Comp Biochem Physiol B. 1984;78(2):379–387. doi: 10.1016/0305-0491(84)90046-4. [DOI] [PubMed] [Google Scholar]
  12. Spielvogel A. M., Farley R. D., Norman A. W. Studies on the mechanism of action of calciferol. V. Turnover time of chick intestinal epithelial cells in relation to the intestinal action of vitamin D. Exp Cell Res. 1972 Oct;74(2):359–366. doi: 10.1016/0014-4827(72)90388-6. [DOI] [PubMed] [Google Scholar]
  13. Szasz G. A kinetic photometric method for serum gamma-glutamyl transpeptidase. Clin Chem. 1969 Feb;15(2):124–136. [PubMed] [Google Scholar]
  14. Thomasset M., Cuisinier-Gleizes P., Mathieu H. 1,25-Dihydroxycholecalciferol: dynamics of the stimulation of duodenal calcium-binding protein, calcium transport and bone calcium mobilization in vitamin D and calcium-deficient rats. FEBS Lett. 1979 Nov 1;107(1):91–94. doi: 10.1016/0014-5793(79)80470-6. [DOI] [PubMed] [Google Scholar]
  15. Thorens B., Roth J., Norman A. W., Perrelet A., Orci L. Immunocytochemical localization of the vitamin D-dependent calcium binding protein in chick duodenum. J Cell Biol. 1982 Jul;94(1):115–122. doi: 10.1083/jcb.94.1.115. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Weiser M. M. Intestinal epithelial cell surface membrane glycoprotein synthesis. I. An indicator of cellular differentiation. J Biol Chem. 1973 Apr 10;248(7):2536–2541. [PubMed] [Google Scholar]
  17. Wilson P. W., Lawson D. E. Incorporation of labelled leucine into alkaline phosphatase in response to 1,25-dihydroxycholecalciferol in chick intestinal brush borders. Eur J Biochem. 1982 Jul;125(3):555–559. doi: 10.1111/j.1432-1033.1982.tb06718.x. [DOI] [PubMed] [Google Scholar]
  18. Zile M., Bunge E. C., Barsness L., Yamada S., Schnoes H. K., DeLuca H. F. Localization of 1,25-dihydroxyvitamin D3 in intestinal nuclei in vivo. Arch Biochem Biophys. 1978 Feb;186(1):15–24. doi: 10.1016/0003-9861(78)90458-7. [DOI] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES