Skip to main content
PMC home page
Biochemical Journal logoLink to Biochemical Journal
. 1985 Jun 1;228(2):505–512. doi: 10.1042/bj2280505

Respiration-dependent uptake of dihydrostreptomycin by Escherichia coli. Its irreversible nature and lack of evidence for a uniport process.

W W Nichols, S N Young
PMCID: PMC1145009  PMID: 2409962

Abstract

The transport of [3H]dihydrostreptomycin into the cytoplasm of Escherichia coli was distinguished, by its respiration-dependent nature, from binding within the cell envelope. 1. Of the radiolabel in the cytoplasm, 70-90% was dissolved in, or quickly equilibrated with, the cytoplasmic aqueous phase because this proportion rapidly left cells treated with toluene or with butan-1-ol. 2. After a period of respiration-dependent uptake of [3H]dihydrostreptomycin, cells were washed repeatedly by centrifugation and resuspension. Radiolabel did not leave the cells at any appreciable rate. 3. Uptake of dihydrostreptomycin (at an exogenous concentration of 1 mg of base/ml) was monitored for 2h to an apparent equilibrium. Then the specific radioactivity of exogenous dihydrostreptomycin was raised without significantly altering its chemical concentration. There was no exchange of radiolabel between the exogenous pool and the cytoplasmic pool. 4. Dihydrostreptomycin was not taken up by respiring, cytoplasm-free membrane vesicles which accumulated L-proline in control experiments. These data support the view that respiration-dependent uptake of dihydrostreptomycin by E. coli is not simply a secondary translocation process such as uniport.

Full text

PDF
508

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ANAND N., DAVIS B. D., ARMITAGE A. K. Uptake of streptomycin by Escherichia coli. Nature. 1960 Jan 2;185:23–24. doi: 10.1038/185023a0. [DOI] [PubMed] [Google Scholar]
  2. Andry K., Bockrath R. C. Dihydrostreptomycin accumulation in E. coli. Nature. 1974 Oct 11;251(5475):534–536. doi: 10.1038/251534a0. [DOI] [PubMed] [Google Scholar]
  3. Bryan L. E., Kwan S. Roles of ribosomal binding, membrane potential, and electron transport in bacterial uptake of streptomycin and gentamicin. Antimicrob Agents Chemother. 1983 Jun;23(6):835–845. doi: 10.1128/aac.23.6.835. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bryan L. E., Van Den Elzen H. M. Effects of membrane-energy mutations and cations on streptomycin and gentamicin accumulation by bacteria: a model for entry of streptomycin and gentamicin in susceptible and resistant bacteria. Antimicrob Agents Chemother. 1977 Aug;12(2):163–177. doi: 10.1128/aac.12.2.163. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Bryan L. E., Van den Elzen H. M. Streptomycin accumulation in susceptible and resistant strains of Escherichia coli and Pseudomonas aeruginosa. Antimicrob Agents Chemother. 1976 Jun;9(6):928–938. doi: 10.1128/aac.9.6.928. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Chang F. N., Flaks J. G. Binding of dihydrostreptomycin to Escherichia coli ribosomes: kinetics of the reaction. Antimicrob Agents Chemother. 1972 Oct;2(4):308–319. doi: 10.1128/aac.2.4.308. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. DAVIS B. D., MINGIOLI E. S. Mutants of Escherichia coli requiring methionine or vitamin B12. J Bacteriol. 1950 Jul;60(1):17–28. doi: 10.1128/jb.60.1.17-28.1950. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. DUBIN D. T., HANCOCK R., DAVIS B. D. THE SEQUENCE OF SOME EFFECTS OF STREPTOMYCIN IN ESCHERICHIA COLI. Biochim Biophys Acta. 1963 Aug 13;74:476–489. doi: 10.1016/0006-3002(63)91390-8. [DOI] [PubMed] [Google Scholar]
  9. Damper P. D., Epstein W. Role of the membrane potential in bacterial resistance to aminoglycoside antibiotics. Antimicrob Agents Chemother. 1981 Dec;20(6):803–808. doi: 10.1128/aac.20.6.803. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Dills S. S., Apperson A., Schmidt M. R., Saier M. H., Jr Carbohydrate transport in bacteria. Microbiol Rev. 1980 Sep;44(3):385–418. doi: 10.1128/mr.44.3.385-418.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. HURWITZ C., ROSANO C. L. Accumulation of label from C14-streptomycin by Escherichia coli. J Bacteriol. 1962 Jun;83:1193–1201. doi: 10.1128/jb.83.6.1193-1201.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Hancock R. E. Aminoglycoside uptake and mode of action--with special reference to streptomycin and gentamicin. I. Antagonists and mutants. J Antimicrob Chemother. 1981 Oct;8(4):249–276. doi: 10.1093/jac/8.4.249. [DOI] [PubMed] [Google Scholar]
  13. Hancock R. E. Aminoglycoside uptake and mode of action-with special reference to streptomycin and gentamicin. II. Effects of aminoglycosides on cells. J Antimicrob Chemother. 1981 Dec;8(6):429–445. doi: 10.1093/jac/8.6.429. [DOI] [PubMed] [Google Scholar]
  14. Höltje J. V. Streptomycin uptake via an inducible polyamine transport system in Escherichia coli. Eur J Biochem. 1978 May 16;86(2):345–351. doi: 10.1111/j.1432-1033.1978.tb12316.x. [DOI] [PubMed] [Google Scholar]
  15. Jackson R. W., DeMoss J. A. Effects of toluene on Escherichia coli. J Bacteriol. 1965 Nov;90(5):1420–1425. doi: 10.1128/jb.90.5.1420-1425.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Kaback H. R. Transport across isolated bacterial cytoplasmic membranes. Biochim Biophys Acta. 1972 Aug 4;265(3):367–416. doi: 10.1016/0304-4157(72)90014-7. [DOI] [PubMed] [Google Scholar]
  17. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  18. Leifer Z., Engel R., Tropp B. E. Transport of 3,4-dihydroxybutyl-1-phosphonate, an analogue of sn-glycerol 3-phosphate. J Bacteriol. 1977 May;130(2):968–971. doi: 10.1128/jb.130.2.968-971.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. McQUILLEN K. The bacterial surface. IV. Effect of streptomycin on the electrophoretic mobility of Escherichia coli and Staphylococcus aureus. Biochim Biophys Acta. 1951 May;7(1):54–60. doi: 10.1016/0006-3002(51)90005-4. [DOI] [PubMed] [Google Scholar]
  20. Mitchell P. Translocations through natural membranes. Adv Enzymol Relat Areas Mol Biol. 1967;29:33–87. doi: 10.1002/9780470122747.ch2. [DOI] [PubMed] [Google Scholar]
  21. SPOTTS C. R., STANIER R. Y. Mechanism of streptomycin action on bacteria: a unitary hypothesis. Nature. 1961 Nov 18;192:633–637. doi: 10.1038/192633a0. [DOI] [PubMed] [Google Scholar]
  22. Tabor C. W., Tabor H. Transport systems for 1,4-diaminobutane, spermidine, and spermine in Escherichia coli. J Biol Chem. 1966 Aug 25;241(16):3714–3723. [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES