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. 1978 Jul;31(7):659–665. doi: 10.1136/jcp.31.7.659

Specific and non-specific folate binding protein in normal and malignant human tissues

R Corrocher 1,2,1, G De Sandre 1,2, A Ambrosetti 1,2, M L Pachor 1,2, L M Bambara 1,2, A V Hoffbrand 1,2
PMCID: PMC1145370  PMID: 670421

Abstract

Binding of tritiated folic acid by supernatants prepared from extracts of normal and leukaemic leucocytes, normal mucosa, and malignant tumours from different parts of the gastrointestinal tract has been measured using Sephadex-gel filtration and albumin-coated charcoal techniques. Non-specific binding (measured by Sephadex G-75 gel filtration) was almost invariably greater than specific binding measured by albumin-coated charcoal separation of bound and unbound folate. In nine normal leucocyte extracts, binding measured by Sephadex G-75 filtration ranged from 1·3 to 18·2 (mean 8·2) pg/mg protein and by albumin-coated charcoal from 1·0 to 14·8 (mean 6·7) pg/mg protein. Raised specific binding was found in the extracts from leucocytes of eight of 14 patients with chronic granulocytic leukaemia, in four substantially so (389, 121, 108, 59·7 pg/mg protein), but was only marginally increased in one of eight cases of acute myeloid leukaemia and in two of five cases of chronic lymphocytic leukaemia. Binding was normal in the extracts of all three cases of acute lymphoblastic leukaemia tested.

Among the tissues of the gastrointestinal tract binding was greatest by the duodenal mucosa and liver. Extracts of carcinoma of the stomach and colon bound greater amounts of 3H-folic acid than the corresponding normal mucosal extracts but the differences were not large. Sephadex G-200 gel chromatography showed more than one binding peak in the extracts of liver and duodenum but only one peak in the other tissues of the gastrointestinal tract, and only one peak, of molecular weight either about 50 000 or over 200 000, in the leucocyte extracts.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Colman N., Herbert V. Total folate binding capacity of normal human plasma, and variations in uremia, cirrhosis, and pregnancy. Blood. 1976 Dec;48(6):911–921. [PubMed] [Google Scholar]
  2. Corrocher R., De Sandre G., Pacor M. L., Hoffbrand A. V. Hepatic protein binding of folate. Clin Sci Mol Med. 1974 Apr;46(4):551–554. doi: 10.1042/cs0460551. [DOI] [PubMed] [Google Scholar]
  3. DaCosta M., Rothenberg S. P., Kamen B. DNA synthesis in chronic myelogenous leukemia cells: comparison of results in cells containing folate binding factor to replicating cells without binder. Blood. 1972 May;39(5):621–627. [PubMed] [Google Scholar]
  4. Fischer C. D., da Costa M., Rothenberg S. P. The heterogeneity and properties of folate binding proteins from chronic myelogenous leukemia cells. Blood. 1975 Dec;46(6):855–867. [PubMed] [Google Scholar]
  5. Gorst D. W., Courtis M., Delamore I. W. Folic acid binding protein in acute myeloid leukaemia. J Clin Pathol. 1976 Jan;29(1):60–62. doi: 10.1136/jcp.29.1.60. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Lanzkowsky P., Erlandson M. E., Bezan A. I. Isolated defect of folic acid absorption associated with mental retardation and cerebral calcification. Blood. 1969 Oct;34(4):452–465. [PubMed] [Google Scholar]
  7. Leslie G. I., Rowe P. B. Folate binding by the brush border membrane proteins of small intestinal epithelial cells. Biochemistry. 1972 Apr 25;11(9):1696–1703. doi: 10.1021/bi00759a026. [DOI] [PubMed] [Google Scholar]
  8. Markkanen T., Pajula R. L., Virtanen S., Himanen P., Järvinen M. Binding of folic acid to serum proteins. V. Tritiated folic acid in the liver. Acta Haematol. 1975;53(6):329–335. doi: 10.1159/000208201. [DOI] [PubMed] [Google Scholar]
  9. Rothenberg S. P. A macromolecular factor in some leukemic cells which binds folic acid. Proc Soc Exp Biol Med. 1970 Feb;133(2):428–432. doi: 10.3181/00379727-133-34489. [DOI] [PubMed] [Google Scholar]
  10. Rothenberg S. P. Application of competitive ligand binding for the radioassay of vitamin B12 and folic acid. Metabolism. 1973 Aug;22(8):1075–1082. doi: 10.1016/0026-0495(73)90226-6. [DOI] [PubMed] [Google Scholar]
  11. Rothenbery S. P., da Costa M. Folate binding proteins and radioassay for folate. Clin Haematol. 1976 Oct;5(3):569–587. [PubMed] [Google Scholar]
  12. Waxman H. A. Congressman Henry A. Waxman. Investor Owned Hosp Rev. 1975 Jun-Jul;8(3):21-3, 29. [PubMed] [Google Scholar]
  13. Waxman S., Schreiber C. Characteristics of folic acid-binding protein in folate-deficient serum. Blood. 1973 Aug;42(2):291–301. [PubMed] [Google Scholar]
  14. Zamierowski M., Wagner C. High molecular weight complexes of folic acid in mammalian tissues. Biochem Biophys Res Commun. 1974 Sep 9;60(1):81–87. doi: 10.1016/0006-291x(74)90175-2. [DOI] [PubMed] [Google Scholar]
  15. Zettner A., Duly P. E. New evidence for a binding principle specific for folates as a normal constituent of human serum. Clin Chem. 1974 Oct;20(10):1313–1319. [PubMed] [Google Scholar]

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