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. 1979 Nov;32(11):1168–1173. doi: 10.1136/jcp.32.11.1168

Identification of viridans streptococci on the Minitek Miniaturised Differentiation System.

Y Holloway, M Schaareman, J Dankert
PMCID: PMC1145919  PMID: 389954

Abstract

A total of 227 strains of viridans streptococci were simultaneously identified on the Minitek Miniaturised System (BBL) and by a conventional method according to Colman and Williams. The Minitek discs were each overlaid with a drop of sterile liquid paraffin, and the trays were incubated in GaSPak jars (BBL) with CO2 generator envelopes. Identification was possible three to four days earlier than with the conventional method. The results were found to be in agreement with the conventional method. Compared to the identification schemes of Cowan and Steel and of Facklam, the results were also in good agreement. Minor differences were found in the number of positive and negative results in those reactions that are variable in all three schemes.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Facklam R. R. Physiological differentiation of viridans streptococci. J Clin Microbiol. 1977 Feb;5(2):184–201. doi: 10.1128/jcm.5.2.184-201.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Finklea P. J., Cole M. S., Sodeman T. M. Clinical evaluation of the minitek differential system for identification of Enterobacteriaceae. J Clin Microbiol. 1976 Nov;4(5):400–404. doi: 10.1128/jcm.4.5.400-404.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Finland M., Barnes M. W. Changing etiology of bacterial endocarditis in the antibacterial era. Experiences at Boston City Hospital 1933-1965. Ann Intern Med. 1970 Mar;72(3):341–348. doi: 10.7326/0003-4819-72-3-341. [DOI] [PubMed] [Google Scholar]
  4. Gibbons R. J., Fitzgerald R. J. Dextran-induced agglutination of Streptococcus mutans, and its potential role in the formation of microbial dental plaques. J Bacteriol. 1969 May;98(2):341–346. doi: 10.1128/jb.98.2.341-346.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hansen S. L., Hardesty D. R., Myers B. M. Evaluation of the BBL Minitek system for the identification of Enterobacteriaceae. Appl Microbiol. 1974 Nov;28(5):798–801. doi: 10.1128/am.28.5.798-801.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hansen S. L., Stewart B. J. Comparison of API and Minitek to Center for Disease Control methods for the biochemical characterization of anaerobes. J Clin Microbiol. 1976 Sep;4(3):227–231. doi: 10.1128/jcm.4.3.227-231.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Horstmeier C., Washington J. A., 2nd Microbiological study of streptococcal bacteremia. Appl Microbiol. 1973 Oct;26(4):589–591. doi: 10.1128/am.26.4.589-591.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Johnson D. H., Rosenthal A., Nadas A. S. A forty-year review of bacterial endocarditis in infancy and childhood. Circulation. 1975 Apr;51(4):581–588. doi: 10.1161/01.cir.51.4.581. [DOI] [PubMed] [Google Scholar]
  9. KRAUS F. W., NICKERSON J. F., PERRY W. I., WALKER A. P. Peroxide and peroxidogenic bacteria in human saliva. J Bacteriol. 1957 Jun;73(6):727–735. doi: 10.1128/jb.73.6.727-735.1957. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kiehn T. E., Brennan K., Ellner P. D. Evaluation of the Minitek system for identification of Enterobacteriaceae. Appl Microbiol. 1974 Oct;28(4):668–671. doi: 10.1128/am.28.4.668-671.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Linke H. A. New method for the isolation of Streptococcus mutans and its differentiation from other oral streptococci. J Clin Microbiol. 1977 Jun;5(6):604–609. doi: 10.1128/jcm.5.6.604-609.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Loesche W. J., Syed S. A. The predominant cultivable flora of carious plaque and carious dentine. Caries Res. 1973;7(3):201–216. doi: 10.1159/000259844. [DOI] [PubMed] [Google Scholar]
  13. Parker M. T., Ball L. C. Streptococci and aerococci associated with systemic infection in man. J Med Microbiol. 1976 Aug;9(3):275–302. doi: 10.1099/00222615-9-3-275. [DOI] [PubMed] [Google Scholar]
  14. Pelletier L. L., Jr, Coyle M., Petersdorf R. Dextran production as a possible virulence factor in streptococcal endocarditis. Proc Soc Exp Biol Med. 1978 Jul;158(3):415–420. doi: 10.3181/00379727-158-40216. [DOI] [PubMed] [Google Scholar]
  15. Schnurr L. P., Ball A. P., Geddes A. M., Gray J., McGhie D. Bacterial endocarditis in England in the 1970's: a review of 70 patients. Q J Med. 1977 Oct;46(184):499–512. [PubMed] [Google Scholar]
  16. Stargel D., Thompson F. S., Phillips S. E., Lombard G. L., Dowell V. R., Jr Modification of the Minitek Miniaturized Differentiation System for characterization of anaerobic bacteria. J Clin Microbiol. 1976 Mar;3(3):291–301. doi: 10.1128/jcm.3.3.291-301.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]

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