Skip to main content
PMC home page
Journal of Clinical Pathology logoLink to Journal of Clinical Pathology
. 1981 Jan;34(1):93–98. doi: 10.1136/jcp.34.1.93

Comparison of ELISA and RIA for measurement of pneumococcal antibodies before and after vaccination with 14-valent pneumococcal capsular polysaccharide vaccine.

M Koskela, M Leinonen
PMCID: PMC1146421  PMID: 7007449

Abstract

Antibody responses to the 14-valent pneumococcal capsular polysaccharide vaccine in children under school age were measured by enzyme-linked immunosorbent assay (ELISA) and radioimmunoassay (RIA). Specific IgG and IgM antibodies were usually detectable by ELISA in the prevaccination sera, and one or both of them increased as a response to the vaccination. Specific IGA antibodies were detected by ELISA in a part of the post-vaccination sera only. The frequency of the IgA responses increased with the age of the children. The correlation of the ELISA results with RIA was good (r from 0.652 to 0.812) except for type 6A (r = 0.471).

Full text

PDF
93

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ammann A. J., Pelger R. J. Determination of antibody to pneumococcal polysaccharides with chromic chloride-treated human red blood cells and indirect hemagglutination. Appl Microbiol. 1972 Nov;24(5):679–683. doi: 10.1128/am.24.5.679-683.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Baker P. J., Stashak P. W., Prescott B. Use of erythrocytes sensitized with purified pneumococcal polysaccharides for the assay of antibody and antibody-producing cells. Appl Microbiol. 1969 Mar;17(3):422–426. doi: 10.1128/am.17.3.422-426.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Berntsson E., Broholm K. A., Kaijser B. Serological diagnosis of pneumococcal disease with enzyme-linked immunosorbent assay (ELISA). Scand J Infect Dis. 1978;10(3):177–181. doi: 10.3109/inf.1978.10.issue-3.04. [DOI] [PubMed] [Google Scholar]
  4. Borgoño J. M., McLean A. A., Vella P. P., Woodhour A. F., Canepa I., Davidson W. L., Hilleman M. R. Vaccination and revaccination with polyvalent pneumococcal polysaccharide vaccines in adults and infants. Proc Soc Exp Biol Med. 1978 Jan;157(1):148–154. doi: 10.3181/00379727-157-40010. [DOI] [PubMed] [Google Scholar]
  5. Butler J. E., Feldbush T. L., McGivern P. L., Stewart N. The enzyme-linked immunosorbent assay (ELISA): a measure of antibody concentration or affinity. Immunochemistry. 1978 Feb;15(2):131–136. doi: 10.1016/0161-5890(78)90053-6. [DOI] [PubMed] [Google Scholar]
  6. Callahan L. T., 3rd, Woodhour A. F., Meeker J. B., Hilleman M. R. Enzyme-linked immunosorbent assay for measurement of antibodies against pneumococcal polysaccharide antigens: comparison with radioimmunoassay. J Clin Microbiol. 1979 Oct;10(4):459–463. doi: 10.1128/jcm.10.4.459-463.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Carenfelt C. Pneumococcal antibodies in maxillary sinus secretion. Scand J Infect Dis. 1977;9(4):277–283. doi: 10.3109/inf.1977.9.issue-4.04. [DOI] [PubMed] [Google Scholar]
  8. Hilleman M. R., McLean A. A., Vella P. P., Weibel R. E., Woodhour A. F. Polyvalent pneumococcal polysaccharide vaccines. Bull World Health Organ. 1978;56(3):371–375. [PMC free article] [PubMed] [Google Scholar]
  9. Howie V. M., Ploussard J. H., Sloyer J. L., Johnston R. B., Jr Immunoglobulins of the middle ear fluid in acute otitis media: relationship to serum immunoglobulin concentrations and bacterial cultures. Infect Immun. 1973 Apr;7(4):589–593. doi: 10.1128/iai.7.4.589-593.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Keren D. F. Enzyme-linked immunosorbent assay for immunoglobulin G and immunoglobulin A antibodies to Shigella flexneri antigens. Infect Immun. 1979 May;24(2):441–448. doi: 10.1128/iai.24.2.441-448.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Leinikki P., Pässilä S. Solid phase antibody assay by means of enzyme conjugated to anti-immunoglobulin. J Clin Pathol. 1976 Dec;29(12):1116–1120. doi: 10.1136/jcp.29.12.1116. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Mouton R. P., Stoop J. W., Ballieux R. E., Mul N. A. Pneumococcal antibodies in IgA of serum and external secretions. Clin Exp Immunol. 1970 Aug;7(2):201–210. [PMC free article] [PubMed] [Google Scholar]
  13. Russell H., Edwards L. R., Wortham E. W., Facklam R. R. Enzyme-linked immunosorbent assay for detection of antibodies against Streptococcus pneumoniae capsular polysaccharides. J Clin Microbiol. 1980 Feb;11(2):198–199. doi: 10.1128/jcm.11.2.198-199.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Sloyer J. L., Jr, Howie V. M., Ploussard J. H., Ammann A. J., Austrian R., Johnston R. B., Jr Immune response to acute otitis media in children. I. Serotypes isolated and serum and middle ear fluid antibody in pneumococcal otitis media. Infect Immun. 1974 Jun;9(6):1028–1032. doi: 10.1128/iai.9.6.1028-1032.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Weibel R. E., Vella P. P., McLean A. A., Woodhour A. F., Davidson W. L., Hilleman M. R. Studies in human subjects of polyvalent pneumococcal vaccines (39894). Proc Soc Exp Biol Med. 1977 Oct;156(1):144–150. doi: 10.3181/00379727-156-39894. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Clinical Pathology are provided here courtesy of BMJ Publishing Group

RESOURCES