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. 1986 Feb 15;234(1):221–223. doi: 10.1042/bj2340221

The affinity of newly synthesized proteoglycan for hyaluronic acid can be enhanced by exposure to mild alkali.

A H Plaas, J D Sandy
PMCID: PMC1146549  PMID: 3707543

Abstract

The affinities for hyaluronic acid of newly synthesized proteoglycan from post-confluent rabbit chondrocyte cultures and purified bovine proteoglycan monomer were compared. In mixtures prepared at pH 6.8 the newly synthesized proteoglycan had the lower affinity; however, in mixtures incubated at pH 8.5 for 24 h before addition of hyaluronic acid, the newly synthesized proteoglycan exhibited a markedly higher affinity than the bovine monomer. The results suggest that proteoglycan secreted without associated link protein [Plaas, Sandy & Muir (1983) Biochem. J. 214, 855-864] has a low affinity for hyaluronate and that this may be increased during subsequent extracellular processing.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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