Skip to main content
Biochemical Journal logoLink to Biochemical Journal
. 1986 Nov 15;240(1):1–12. doi: 10.1042/bj2400001

The purification of eukaryotic polypeptides synthesized in Escherichia coli.

F A Marston
PMCID: PMC1147368  PMID: 3548705

Full text

PDF
4

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adams J. M. On the release of the formyl group from nascent protein. J Mol Biol. 1968 May 14;33(3):571–589. doi: 10.1016/0022-2836(68)90307-0. [DOI] [PubMed] [Google Scholar]
  2. Adari H. Y., Rose K., Williams K. R., Konigsberg W. H., Lin T. C., Spicer E. K. Cloning, nucleotide sequence, and overexpression of the bacteriophage T4 regA gene. Proc Natl Acad Sci U S A. 1985 Apr;82(7):1901–1905. doi: 10.1073/pnas.82.7.1901. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Allen G., Paynter C. A., Winther M. D. Production of epidermal growth factor in Escherichia coli from a synthetic gene. J Cell Sci Suppl. 1985;3:29–38. doi: 10.1242/jcs.1985.supplement_3.4. [DOI] [PubMed] [Google Scholar]
  4. Anfinsen C. B. Principles that govern the folding of protein chains. Science. 1973 Jul 20;181(4096):223–230. doi: 10.1126/science.181.4096.223. [DOI] [PubMed] [Google Scholar]
  5. Arakawa T., Alton N. K., Hsu Y. R. Preparation and characterization of recombinant DNA-derived human interferon-gamma. J Biol Chem. 1985 Nov 25;260(27):14435–14439. [PubMed] [Google Scholar]
  6. Boss M. A., Kenten J. H., Wood C. R., Emtage J. S. Assembly of functional antibodies from immunoglobulin heavy and light chains synthesised in E. coli. Nucleic Acids Res. 1984 May 11;12(9):3791–3806. doi: 10.1093/nar/12.9.3791. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Botterman J., Zabeau M. High-level production of the EcoRI endonuclease under the control of the pL promoter of bacteriophage lambda. Gene. 1985;37(1-3):229–239. doi: 10.1016/0378-1119(85)90277-x. [DOI] [PubMed] [Google Scholar]
  8. Brosius J. Toxicity of an overproduced foreign gene product in Escherichia coli and its use in plasmid vectors for the selection of transcription terminators. Gene. 1984 Feb;27(2):161–172. doi: 10.1016/0378-1119(84)90137-9. [DOI] [PubMed] [Google Scholar]
  9. Cabilly S., Riggs A. D., Pande H., Shively J. E., Holmes W. E., Rey M., Perry L. J., Wetzel R., Heyneker H. L. Generation of antibody activity from immunoglobulin polypeptide chains produced in Escherichia coli. Proc Natl Acad Sci U S A. 1984 Jun;81(11):3273–3277. doi: 10.1073/pnas.81.11.3273. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Chan S. J., Weiss J., Konrad M., White T., Bahl C., Yu S. D., Marks D., Steiner D. F. Biosynthesis and periplasmic segregation of human proinsulin in Escherichia coli. Proc Natl Acad Sci U S A. 1981 Sep;78(9):5401–5405. doi: 10.1073/pnas.78.9.5401. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Chang Y., Bjeldanes L. F. Effects of dietary R-goitrin on hepatic and intestinal glutathione S-transferase, microsomal epoxide hydratase and ethoxycoumarin O-deethylase activities in the rat. Food Chem Toxicol. 1985 Oct;23(10):905–909. doi: 10.1016/0278-6915(85)90106-1. [DOI] [PubMed] [Google Scholar]
  12. Cheng Y. S. Increased cell buoyant densities of protein overproducing Escherichia coli cells. Biochem Biophys Res Commun. 1983 Feb 28;111(1):104–111. doi: 10.1016/s0006-291x(83)80123-5. [DOI] [PubMed] [Google Scholar]
  13. Cohen S. N., Chang A. C., Boyer H. W., Helling R. B. Construction of biologically functional bacterial plasmids in vitro. Proc Natl Acad Sci U S A. 1973 Nov;70(11):3240–3244. doi: 10.1073/pnas.70.11.3240. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Courtney M., Buchwalder A., Tessier L. H., Jaye M., Benavente A., Balland A., Kohli V., Lathe R., Tolstoshev P., Lecocq J. P. High-level production of biologically active human alpha 1-antitrypsin in Escherichia coli. Proc Natl Acad Sci U S A. 1984 Feb;81(3):669–673. doi: 10.1073/pnas.81.3.669. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Dallas W. S., Falkow S. Amino acid sequence homology between cholera toxin and Escherichia coli heat-labile toxin. Nature. 1980 Dec 4;288(5790):499–501. doi: 10.1038/288499a0. [DOI] [PubMed] [Google Scholar]
  16. De Maeyer E., Skup D., Prasad K. S., De Maeyer-Guignard J., Williams B., Meacock P., Sharpe G., Pioli D., Hennam J., Schuch W. Expression of a chemically synthesized human alpha 1 interferon gene. Proc Natl Acad Sci U S A. 1982 Jul;79(14):4256–4259. doi: 10.1073/pnas.79.14.4256. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Devos R., Plaetinck G., Cheroutre H., Simons G., Degrave W., Tavernier J., Remaut E., Fiers W. Molecular cloning of human interleukin 2 cDNA and its expression in E. coli. Nucleic Acids Res. 1983 Jul 11;11(13):4307–4323. doi: 10.1093/nar/11.13.4307. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Dowbenko D. J., Bell J. R., Benton C. V., Groopman J. E., Nguyen H., Vetterlein D., Capon D. J., Lasky L. A. Bacterial expression of the acquired immunodeficiency syndrome retrovirus p24 gag protein and its use as a diagnostic reagent. Proc Natl Acad Sci U S A. 1985 Nov;82(22):7748–7752. doi: 10.1073/pnas.82.22.7748. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Edge M. D., Camble R. Interferon synthesis by micro-organisms. Biotechnol Genet Eng Rev. 1984;2:215–252. doi: 10.1080/02648725.1984.10647800. [DOI] [PubMed] [Google Scholar]
  20. Emr S. D., Hedgpeth J., Clément J. M., Silhavy T. J., Hofnung M. Sequence analysis of mutations that prevent export of lambda receptor, an Escherichia coli outer membrane protein. Nature. 1980 May 8;285(5760):82–85. doi: 10.1038/285082a0. [DOI] [PubMed] [Google Scholar]
  21. Fahey R. C., Hunt J. S., Windham G. C. On the cysteine and cystine content of proteins. Differences between intracellular and extracellular proteins. J Mol Evol. 1977 Nov 25;10(2):155–160. doi: 10.1007/BF01751808. [DOI] [PubMed] [Google Scholar]
  22. Freudl R., Schwarz H., Klose M., Movva N. R., Henning U. The nature of information, required for export and sorting, present within the outer membrane protein OmpA of Escherichia coli K-12. EMBO J. 1985 Dec 16;4(13A):3593–3598. doi: 10.1002/j.1460-2075.1985.tb04122.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. George H. J., L'Italien J. J., Pilacinski W. P., Glassman D. L., Krzyzek R. A. High-level expression in Escherichia coli of biologically active bovine growth hormone. DNA. 1985 Aug;4(4):273–281. doi: 10.1089/dna.1985.4.273. [DOI] [PubMed] [Google Scholar]
  24. Ghrayeb J., Kimura H., Takahara M., Hsiung H., Masui Y., Inouye M. Secretion cloning vectors in Escherichia coli. EMBO J. 1984 Oct;3(10):2437–2442. doi: 10.1002/j.1460-2075.1984.tb02151.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Goeddel D. V., Heyneker H. L., Hozumi T., Arentzen R., Itakura K., Yansura D. G., Ross M. J., Miozzari G., Crea R., Seeburg P. H. Direct expression in Escherichia coli of a DNA sequence coding for human growth hormone. Nature. 1979 Oct 18;281(5732):544–548. doi: 10.1038/281544a0. [DOI] [PubMed] [Google Scholar]
  26. Goeddel D. V., Kleid D. G., Bolivar F., Heyneker H. L., Yansura D. G., Crea R., Hirose T., Kraszewski A., Itakura K., Riggs A. D. Expression in Escherichia coli of chemically synthesized genes for human insulin. Proc Natl Acad Sci U S A. 1979 Jan;76(1):106–110. doi: 10.1073/pnas.76.1.106. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Goff S. A., Goldberg A. L. Production of abnormal proteins in E. coli stimulates transcription of lon and other heat shock genes. Cell. 1985 Jun;41(2):587–595. doi: 10.1016/s0092-8674(85)80031-3. [DOI] [PubMed] [Google Scholar]
  28. Gray G. L., Baldridge J. S., McKeown K. S., Heyneker H. L., Chang C. N. Periplasmic production of correctly processed human growth hormone in Escherichia coli: natural and bacterial signal sequences are interchangeable. Gene. 1985;39(2-3):247–254. doi: 10.1016/0378-1119(85)90319-1. [DOI] [PubMed] [Google Scholar]
  29. Gray P. W., Aggarwal B. B., Benton C. V., Bringman T. S., Henzel W. J., Jarrett J. A., Leung D. W., Moffat B., Ng P., Svedersky L. P. Cloning and expression of cDNA for human lymphotoxin, a lymphokine with tumour necrosis activity. Nature. 1984 Dec 20;312(5996):721–724. doi: 10.1038/312721a0. [DOI] [PubMed] [Google Scholar]
  30. Gribskov M., Burgess R. R. Overexpression and purification of the sigma subunit of Escherichia coli RNA polymerase. Gene. 1983 Dec;26(2-3):109–118. doi: 10.1016/0378-1119(83)90180-4. [DOI] [PubMed] [Google Scholar]
  31. Henning U., Cole S. T., Bremer E., Hindennach I., Schaller H. Gene fusions using the ompA gene coding for a major outer-membrane protein of Escherichia coli K12. Eur J Biochem. 1983 Nov 2;136(2):233–240. doi: 10.1111/j.1432-1033.1983.tb07732.x. [DOI] [PubMed] [Google Scholar]
  32. Hsu Y. R., Arakawa T. Structural studies on acid unfolding and refolding of recombinant human interferon gamma. Biochemistry. 1985 Dec 31;24(27):7959–7963. doi: 10.1021/bi00348a018. [DOI] [PubMed] [Google Scholar]
  33. Itakura K., Hirose T., Crea R., Riggs A. D., Heyneker H. L., Bolivar F., Boyer H. W. Expression in Escherichia coli of a chemically synthesized gene for the hormone somatostatin. Science. 1977 Dec 9;198(4321):1056–1063. doi: 10.1126/science.412251. [DOI] [PubMed] [Google Scholar]
  34. Jacob C. O., Leitner M., Zamir A., Salomon D., Arnon R. Priming immunization against cholera toxin and E. coli heat-labile toxin by a cholera toxin short peptide-beta-galactosidase hybrid synthesized in E. coli. EMBO J. 1985 Dec 1;4(12):3339–3343. doi: 10.1002/j.1460-2075.1985.tb04086.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  35. Jacobs P., Cravador A., Loriau R., Brockly F., Colau B., Chuchana P., van Elsen A., Herzog A., Bollen A. Molecular cloning, sequencing, and expression in Escherichia coli of human preprourokinase cDNA. DNA. 1985 Apr;4(2):139–146. doi: 10.1089/dna.1985.4.139. [DOI] [PubMed] [Google Scholar]
  36. Katsoyannis P. G., Tometsko A., Zalut C. Insulin peptides. XII. Human insulin generation by combination of synthetic A and B chains. J Am Chem Soc. 1966 Jan 5;88(1):166–167. doi: 10.1021/ja00953a033. [DOI] [PubMed] [Google Scholar]
  37. Kleid D. G., Yansura D., Small B., Dowbenko D., Moore D. M., Grubman M. J., McKercher P. D., Morgan D. O., Robertson B. H., Bachrach H. L. Cloned viral protein vaccine for foot-and-mouth disease: responses in cattle and swine. Science. 1981 Dec 4;214(4525):1125–1129. doi: 10.1126/science.6272395. [DOI] [PubMed] [Google Scholar]
  38. Klump W., Marquardt O., Hofschneider P. H. Biologically active protease of foot and mouth disease virus is expressed from cloned viral cDNA in Escherichia coli. Proc Natl Acad Sci U S A. 1984 Jun;81(11):3351–3355. doi: 10.1073/pnas.81.11.3351. [DOI] [PMC free article] [PubMed] [Google Scholar]
  39. Kohr W. J., Keck R., Harkins R. N. Characterization of intact and trypsin-digested biosynthetic human growth hormone by high-pressure liquid chromatography. Anal Biochem. 1982 May 15;122(2):348–359. doi: 10.1016/0003-2697(82)90294-9. [DOI] [PubMed] [Google Scholar]
  40. Kotewicz M. L., D'Alessio J. M., Driftmier K. M., Blodgett K. P., Gerard G. F. Cloning and overexpression of Moloney murine leukemia virus reverse transcriptase in Escherichia coli. Gene. 1985;35(3):249–258. doi: 10.1016/0378-1119(85)90003-4. [DOI] [PubMed] [Google Scholar]
  41. Liang S. M., Allet B., Rose K., Hirschi M., Liang C. M., Thatcher D. R. Characterization of human interleukin 2 derived from Escherichia coli. Biochem J. 1985 Jul 15;229(2):429–439. doi: 10.1042/bj2290429. [DOI] [PMC free article] [PubMed] [Google Scholar]
  42. Liu F. T., Albrandt K. A., Bry C. G., Ishizaka T. Expression of a biologically active fragment of human IgE epsilon chain in Escherichia coli. Proc Natl Acad Sci U S A. 1984 Sep;81(17):5369–5373. doi: 10.1073/pnas.81.17.5369. [DOI] [PMC free article] [PubMed] [Google Scholar]
  43. London J., Skrzynia C., Goldberg M. E. Renaturation of Escherichia coli tryptophanase after exposure to 8 M urea. Evidence for the existence of nucleation centers. Eur J Biochem. 1974 Sep 1;47(2):409–415. doi: 10.1111/j.1432-1033.1974.tb03707.x. [DOI] [PubMed] [Google Scholar]
  44. Lord S. T. Expression of a cloned human fibrinogen cDNA in Escherichia coli: synthesis of an A alpha polypeptide. DNA. 1985 Feb;4(1):33–38. doi: 10.1089/dna.1985.4.33. [DOI] [PubMed] [Google Scholar]
  45. Malkovský M., Medawar P. B., Thatcher D. R., Toy J., Hunt R., Rayfield L. S., Doré C. Acquired immunological tolerance of foreign cells is impaired by recombinant interleukin 2 or vitamin A acetate. Proc Natl Acad Sci U S A. 1985 Jan;82(2):536–538. doi: 10.1073/pnas.82.2.536. [DOI] [PMC free article] [PubMed] [Google Scholar]
  46. Mandecki W., Mollison K. W., Bolling T. J., Powell B. S., Carter G. W., Fox J. L. Chemical synthesis of a gene encoding the human complement fragment C5a and its expression in Escherichia coli. Proc Natl Acad Sci U S A. 1985 Jun;82(11):3543–3547. doi: 10.1073/pnas.82.11.3543. [DOI] [PMC free article] [PubMed] [Google Scholar]
  47. Michaelis S., Beckwith J. Mechanism of incorporation of cell envelope proteins in Escherichia coli. Annu Rev Microbiol. 1982;36:435–465. doi: 10.1146/annurev.mi.36.100182.002251. [DOI] [PubMed] [Google Scholar]
  48. Müller D., Hughes C., Goebel W. Relationship between plasmid and chromosomal hemolysin determinants of Escherichia coli. J Bacteriol. 1983 Feb;153(2):846–851. doi: 10.1128/jb.153.2.846-851.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  49. Nagahari K., Kanaya S., Munakata K., Aoyagi Y., Mizushima S. Secretion into the culture medium of a foreign gene product from Escherichia coli: use of the ompF gene for secretion of human beta-endorphin. EMBO J. 1985 Dec 16;4(13A):3589–3592. doi: 10.1002/j.1460-2075.1985.tb04121.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  50. Nagai K., Perutz M. F., Poyart C. Oxygen binding properties of human mutant hemoglobins synthesized in Escherichia coli. Proc Natl Acad Sci U S A. 1985 Nov;82(21):7252–7255. doi: 10.1073/pnas.82.21.7252. [DOI] [PMC free article] [PubMed] [Google Scholar]
  51. Nishimori K., Shimizu N., Kawaguchi Y., Hidaka M., Uozumi T., Beppu T. Expression of cloned calf prochymosin cDNA under control of the tryptophan promoter. Gene. 1984 Jul-Aug;29(1-2):41–49. doi: 10.1016/0378-1119(84)90164-1. [DOI] [PubMed] [Google Scholar]
  52. Nossal N. G., Heppel L. A. The release of enzymes by osmotic shock from Escherichia coli in exponential phase. J Biol Chem. 1966 Jul 10;241(13):3055–3062. [PubMed] [Google Scholar]
  53. Offensperger W., Wahl S., Neurath A. R., Price P., Strick N., Kent S. B., Christman J. K., Acs G. Expression in Escherichia coli of a cloned DNA sequence encoding the pre-S2 region of hepatitis B virus. Proc Natl Acad Sci U S A. 1985 Nov;82(22):7540–7544. doi: 10.1073/pnas.82.22.7540. [DOI] [PMC free article] [PubMed] [Google Scholar]
  54. Oka T., Sakamoto S., Miyoshi K., Fuwa T., Yoda K., Yamasaki M., Tamura G., Miyake T. Synthesis and secretion of human epidermal growth factor by Escherichia coli. Proc Natl Acad Sci U S A. 1985 Nov;82(21):7212–7216. doi: 10.1073/pnas.82.21.7212. [DOI] [PMC free article] [PubMed] [Google Scholar]
  55. Olson K. C., Fenno J., Lin N., Harkins R. N., Snider C., Kohr W. H., Ross M. J., Fodge D., Prender G., Stebbing N. Purified human growth hormone from E. coli is biologically active. Nature. 1981 Oct 1;293(5831):408–411. doi: 10.1038/293408a0. [DOI] [PubMed] [Google Scholar]
  56. POLLOCK M. R., RICHMOND M. H. Low cyst(e)ine content of bacterial extracellular proteins: its possible physiological significance. Nature. 1962 May 5;194:446–449. doi: 10.1038/194446a0. [DOI] [PubMed] [Google Scholar]
  57. Pennica D., Hayflick J. S., Bringman T. S., Palladino M. A., Goeddel D. V. Cloning and expression in Escherichia coli of the cDNA for murine tumor necrosis factor. Proc Natl Acad Sci U S A. 1985 Sep;82(18):6060–6064. doi: 10.1073/pnas.82.18.6060. [DOI] [PMC free article] [PubMed] [Google Scholar]
  58. Pennica D., Nedwin G. E., Hayflick J. S., Seeburg P. H., Derynck R., Palladino M. A., Kohr W. J., Aggarwal B. B., Goeddel D. V. Human tumour necrosis factor: precursor structure, expression and homology to lymphotoxin. Nature. 1984 Dec 20;312(5996):724–729. doi: 10.1038/312724a0. [DOI] [PubMed] [Google Scholar]
  59. Pollitt S., Zalkin H. Role of primary structure and disulfide bond formation in beta-lactamase secretion. J Bacteriol. 1983 Jan;153(1):27–32. doi: 10.1128/jb.153.1.27-32.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  60. Prouty W. F., Goldberg A. L. Fate of abnormal proteins in E. coli accumulation in intracellular granules before catabolism. Nat New Biol. 1972 Nov 29;240(100):147–150. doi: 10.1038/newbio240147a0. [DOI] [PubMed] [Google Scholar]
  61. Prouty W. F., Karnovsky M. J., Goldberg A. L. Degradation of abnormal proteins in Escherichia coli. Formation of protein inclusions in cells exposed to amino acid analogs. J Biol Chem. 1975 Feb 10;250(3):1112–1122. [PubMed] [Google Scholar]
  62. Schoemaker J. M., Brasnett A. H., Marston F. A. Examination of calf prochymosin accumulation in Escherichia coli: disulphide linkages are a structural component of prochymosin-containing inclusion bodies. EMBO J. 1985 Mar;4(3):775–780. doi: 10.1002/j.1460-2075.1985.tb03696.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  63. Seeburg P. H., Shine J., Martial J. A., Ivarie R. D., Morris J. A., Ullrich A., Baxter J. D., Goodman H. M. Synthesis of growth hormone by bacteria. Nature. 1978 Dec 21;276(5690):795–798. doi: 10.1038/276795a0. [DOI] [PubMed] [Google Scholar]
  64. Sekine S., Mizukami T., Nishi T., Kuwana Y., Saito A., Sato M., Itoh S., Kawauchi H. Cloning and expression of cDNA for salmon growth hormone in Escherichia coli. Proc Natl Acad Sci U S A. 1985 Jul;82(13):4306–4310. doi: 10.1073/pnas.82.13.4306. [DOI] [PMC free article] [PubMed] [Google Scholar]
  65. Shen S. H. Multiple joined genes prevent product degradation in Escherichia coli. Proc Natl Acad Sci U S A. 1984 Aug;81(15):4627–4631. doi: 10.1073/pnas.81.15.4627. [DOI] [PMC free article] [PubMed] [Google Scholar]
  66. Shine J., Fettes I., Lan N. C., Roberts J. L., Baxter J. D. Expression of cloned beta-endorphin gene sequences by Escherichia coli. Nature. 1980 Jun 12;285(5765):456–463. doi: 10.1038/285456a0. [DOI] [PubMed] [Google Scholar]
  67. Silhavy T. J., Benson S. A., Emr S. D. Mechanisms of protein localization. Microbiol Rev. 1983 Sep;47(3):313–344. doi: 10.1128/mr.47.3.313-344.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  68. Simons G., Remaut E., Allet B., Devos R., Fiers W. High-level expression of human interferon gamma in Escherichia coli under control of the pL promoter of bacteriophage lambda. Gene. 1984 Apr;28(1):55–64. doi: 10.1016/0378-1119(84)90087-8. [DOI] [PubMed] [Google Scholar]
  69. Staehelin T., Hobbs D. S., Kung H., Pestka S. Purification of recombinant human leukocyte interferon (IFLrA) with monoclonal antibodies. Methods Enzymol. 1981;78(Pt A):505–512. doi: 10.1016/0076-6879(81)78162-x. [DOI] [PubMed] [Google Scholar]
  70. Straus D., Gilbert W. Chicken triosephosphate isomerase complements an Escherichia coli deficiency. Proc Natl Acad Sci U S A. 1985 Apr;82(7):2014–2018. doi: 10.1073/pnas.82.7.2014. [DOI] [PMC free article] [PubMed] [Google Scholar]
  71. Sung W. L., Yao F. L., Zahab D. M., Narang S. A. Short synthetic oligodeoxyribonucleotide leader sequences enhance accumulation of human proinsulin synthesized in Escherichia coli. Proc Natl Acad Sci U S A. 1986 Feb;83(3):561–565. doi: 10.1073/pnas.83.3.561. [DOI] [PMC free article] [PubMed] [Google Scholar]
  72. Szoka P. R., Schreiber A. B., Chan H., Murthy J. A general method for retrieving the components of a genetically engineered fusion protein. DNA. 1986 Feb;5(1):11–20. doi: 10.1089/dna.1986.5.11. [DOI] [PubMed] [Google Scholar]
  73. Talmadge K., Gilbert W. Cellular location affects protein stability in Escherichia coli. Proc Natl Acad Sci U S A. 1982 Mar;79(6):1830–1833. doi: 10.1073/pnas.79.6.1830. [DOI] [PMC free article] [PubMed] [Google Scholar]
  74. Tommassen J., van Tol H., Lugtenberg B. The ultimate localization of an outer membrane protein of Escherichia coli K-12 is not determined by the signal sequence. EMBO J. 1983;2(8):1275–1279. doi: 10.1002/j.1460-2075.1983.tb01581.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  75. Varadarajan R., Szabo A., Boxer S. G. Cloning, expression in Escherichia coli, and reconstitution of human myoglobin. Proc Natl Acad Sci U S A. 1985 Sep;82(17):5681–5684. doi: 10.1073/pnas.82.17.5681. [DOI] [PMC free article] [PubMed] [Google Scholar]
  76. Villa-Komaroff L., Efstratiadis A., Broome S., Lomedico P., Tizard R., Naber S. P., Chick W. L., Gilbert W. A bacterial clone synthesizing proinsulin. Proc Natl Acad Sci U S A. 1978 Aug;75(8):3727–3731. doi: 10.1073/pnas.75.8.3727. [DOI] [PMC free article] [PubMed] [Google Scholar]
  77. Vogel T., Weisgraber K. H., Zeevi M. I., Ben-Artzi H., Levanon A. Z., Rall S. C., Jr, Innerarity T. L., Hui D. Y., Taylor J. M., Kanner D. Human apolipoprotein E expression in Escherichia coli: structural and functional identity of the bacterially produced protein with plasma apolipoprotein E. Proc Natl Acad Sci U S A. 1985 Dec;82(24):8696–8700. doi: 10.1073/pnas.82.24.8696. [DOI] [PMC free article] [PubMed] [Google Scholar]
  78. Waldman A. S., Haeusslein E., Milman G. Purification and characterization of herpes simplex virus (type 1) thymidine kinase produced in Escherichia coli by a high efficiency expression plasmid utilizing a lambda PL promoter and cI857 temperature-sensitive repressor. J Biol Chem. 1983 Oct 10;258(19):11571–11575. [PubMed] [Google Scholar]
  79. Whitehorn E. A., Livak K. J., Petteway S. R., Jr The effects of hybrid ribosome-binding-site variants on the expression of human interferon-beta in Escherichia coli. Gene. 1985;36(3):375–379. doi: 10.1016/0378-1119(85)90194-5. [DOI] [PubMed] [Google Scholar]
  80. Williams D. C., Van Frank R. M., Muth W. L., Burnett J. P. Cytoplasmic inclusion bodies in Escherichia coli producing biosynthetic human insulin proteins. Science. 1982 Feb 5;215(4533):687–689. doi: 10.1126/science.7036343. [DOI] [PubMed] [Google Scholar]
  81. Zemel-Dreasen O., Zamir A. Secretion and processing of an immunoglobulin light chain in Escherichia coli. Gene. 1984 Mar;27(3):315–322. doi: 10.1016/0378-1119(84)90076-3. [DOI] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES