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. 1986 Dec 1;240(2):495–502. doi: 10.1042/bj2400495

Preliminary evidence for a glucan acceptor in the yeast Candida albicans.

E Andaluz, A Guillén, G Larriba
PMCID: PMC1147443  PMID: 2949741

Abstract

Two membrane preparation containing glucan synthase activity were obtained by lysis of regenerating sphaeroplasts (enzyme A) or mechanical breakage (enzyme B) of yeast (Candida albicans) cells. The reaction products of both enzymes (glucans A and B respectively) were characterized as linear beta-1,3-linked glucans on the basis of chemical and enzymic analysis. In addition, two pools of glucan could be distinguished in glucan A preparations on the basis of their susceptibility to an exoglucanase. In no case were the reaction products synthesized de novo; rather the radioactive chains were added to the non-reducing end of non-radioactive preformed glucan chains or to an acceptor of a different nature. At least some of the performed chains of glucan A, but not those of glucan B, showed a free reducing terminal. Glucan A preparations were endowed with endoglucanase activity, which, under appropriate conditions, released glucose, laminaribiose and laminaritriose. These sugars were also found in cell-wall autolysates. On the basis of the origin of both enzyme preparations it is suggested that glucan molecules are synthesized while they are bound to a non-glucan acceptor that is subsequently excised, presumably by cell-wall-associated glucanases.

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Selected References

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  1. Aon M. A., Curtino J. A. Evidence for the glycoprotein nature of retina glycogen. Eur J Biochem. 1984 May 2;140(3):557–566. doi: 10.1111/j.1432-1033.1984.tb08138.x. [DOI] [PubMed] [Google Scholar]
  2. Aon M. A., Curtino J. A. Protein-bound glycogen is linked to tyrosine residues. Biochem J. 1985 Jul 1;229(1):269–272. doi: 10.1042/bj2290269. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Blumenfeld M. L., Krisman C. R. The initiation of glycogen biosynthesis in rat heart. Studies with a purified preparation. J Biol Chem. 1985 Sep 25;260(21):11560–11566. [PubMed] [Google Scholar]
  4. Gopal P. K., Shepherd M. G., Sullivan P. A. Analysis of wall glucans from yeast, hyphal and germ-tube forming cells of Candida albicans. J Gen Microbiol. 1984 Dec;130(12):3295–3301. doi: 10.1099/00221287-130-12-3295. [DOI] [PubMed] [Google Scholar]
  5. Gopal P., Sullivan P. A., Shepherd M. G. Isolation and structure of glucan from regenerating spheroplasts of Candida albicans. J Gen Microbiol. 1984 May;130(5):1217–1225. doi: 10.1099/00221287-130-5-1217. [DOI] [PubMed] [Google Scholar]
  6. Guillén A., Leal F., Andaluz E., Larriba G. Endogenous factors that modulate yeast glucan synthetase in cell-free extracts. Biochim Biophys Acta. 1985 Oct 17;842(2-3):151–161. doi: 10.1016/0304-4165(85)90197-7. [DOI] [PubMed] [Google Scholar]
  7. Hernández L. M., Olivero I., Ramírez M., Larriba G. Accumulation of exoglucanase activity in yeast secretory mutants blocked at the endoplasmic reticulum level. FEBS Lett. 1986 Feb 17;196(2):291–295. doi: 10.1016/0014-5793(86)80265-4. [DOI] [PubMed] [Google Scholar]
  8. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  9. López-Romero E., Ruiz-Herrera J. Biosynthesis of beta-glucans by cell-free extracts from Saccharomyces cerevisiae. Biochim Biophys Acta. 1977 Dec 22;500(2):372–384. doi: 10.1016/0304-4165(77)90028-9. [DOI] [PubMed] [Google Scholar]
  10. Manners D. J., Masson A. J., Patterson J. C. The structure of a beta-(1 leads to 3)-D-glucan from yeast cell walls. Biochem J. 1973 Sep;135(1):19–30. doi: 10.1042/bj1350019. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Nelson T. E., Johnson J., Jr, Jantzen E., Kirkwood S. Action pattern and specificity of an exo-beta-(1--3)-D-glucanase from basidiomycetes species QM 806. J Biol Chem. 1969 Nov 10;244(21):5972–5980. [PubMed] [Google Scholar]
  12. Nelson T. E. The attack mechanism of an exo-1,3-beta-glucosidase from Basidiomycete sp. QM 806. Biochim Biophys Acta. 1975 Jan 23;377(1):139–145. doi: 10.1016/0005-2744(75)90294-6. [DOI] [PubMed] [Google Scholar]
  13. Notario V. beta-Glucanases from Candida albicans: purification, characterization and the nature of their attachment to cell wall components. J Gen Microbiol. 1982 Apr;128(4):747–759. doi: 10.1099/00221287-128-4-747. [DOI] [PubMed] [Google Scholar]
  14. Orlean P. A. (1,3)-beta-D-Glucan synthase from budding and filamentous cultures of the dimorphic fungus Candida albicans. Eur J Biochem. 1982 Oct;127(2):397–403. doi: 10.1111/j.1432-1033.1982.tb06885.x. [DOI] [PubMed] [Google Scholar]
  15. REESE E. T., MANDELS M. Beta-D-1, 3 Glucanases in fungi. Can J Microbiol. 1959 Apr;5(2):173–185. doi: 10.1139/m59-022. [DOI] [PubMed] [Google Scholar]
  16. Rodriguez I. R., Whelan W. J. A novel glycosyl-amino acid linkage: rabbit-muscle glycogen is covalently linked to a protein via tyrosine. Biochem Biophys Res Commun. 1985 Oct 30;132(2):829–836. doi: 10.1016/0006-291x(85)91206-9. [DOI] [PubMed] [Google Scholar]
  17. SMOGYI M. Notes on sugar determination. J Biol Chem. 1952 Mar;195(1):19–23. [PubMed] [Google Scholar]
  18. Shematek E. M., Braatz J. A., Cabib E. Biosynthesis of the yeast cell wall. I. Preparation and properties of beta-(1 leads to 3)glucan synthetase. J Biol Chem. 1980 Feb 10;255(3):888–894. [PubMed] [Google Scholar]
  19. TREVELYAN W. E., PROCTER D. P., HARRISON J. S. Detection of sugars on paper chromatograms. Nature. 1950 Sep 9;166(4219):444–445. doi: 10.1038/166444b0. [DOI] [PubMed] [Google Scholar]
  20. Walker G. J., Hare M. D. Metabolism of the polysaccharides of human dental plaque. Part II. Purification and properties of Cladosporium resinae (1 leads to 3)-alpha-D-glucanase, and the enzymic hydrolysis of glucans synthesised by extracellular D-glucosyltransferases of oral streptococci. Carbohydr Res. 1977 Oct;58(2):415–432. doi: 10.1016/s0008-6215(00)84368-2. [DOI] [PubMed] [Google Scholar]

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