Skip to main content
PMC home page
Biochemical Journal logoLink to Biochemical Journal
. 1986 Dec 15;240(3):885–889. doi: 10.1042/bj2400885

Tissue distribution of rat glutathione transferase subunit 7, a hepatoma marker.

S E Pemble, J B Taylor, B Ketterer
PMCID: PMC1147502  PMID: 3827877

Abstract

Polyadenylated RNA isolated from NN-dimethyl-4-aminoazobenzene-induced rat hepatoma was used to prepare a cDNA library in lambda gt10. Full-length clones complementary to mRNA coding for glutathione transferase subunit 7 were isolated and one of these clones (pGSTr7) was fully characterized. In Northern blot analysis, mRNA hybridizing to 32P-labelled pGSTr7 was found in poly(A)-containing RNA isolated from seven normal rat tissues but not from testis and liver. A similar hybridizing mRNA species was also detected in human placental mRNA. The same probe, used in a Southern blot analysis of genomic DNA, suggests the presence of a multigene family in the rat.

Full text

PDF
886

Images in this article

887Fig. 1.
on p.887
888Fig. 2.
on p.888

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Beale D., Ketterer B., Carne T., Meyer D., Taylor J. B. Evidence that the Ya and Yc subunits of glutathione transferase B (ligandin) are the products of separate genes. Eur J Biochem. 1982 Sep 1;126(3):459–463. doi: 10.1111/j.1432-1033.1982.tb06802.x. [DOI] [PubMed] [Google Scholar]
  2. Beale D., Meyer D. J., Taylor J. B., Ketterer B. Evidence that the Yb subunits of hepatic glutathione transferases represent two different but related families of polypeptides. Eur J Biochem. 1983 Dec 1;137(1-2):125–129. doi: 10.1111/j.1432-1033.1983.tb07805.x. [DOI] [PubMed] [Google Scholar]
  3. Benton W. D., Davis R. W. Screening lambdagt recombinant clones by hybridization to single plaques in situ. Science. 1977 Apr 8;196(4286):180–182. doi: 10.1126/science.322279. [DOI] [PubMed] [Google Scholar]
  4. Craig R. K., Hall L., Parker D., Campbell P. N. The construction, identification and partial characterization of plasmids containing guinea-pig milk protein complementary DNA sequences. Biochem J. 1981 Mar 15;194(3):989–998. doi: 10.1042/bj1940989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Deeley R. G., Gordon J. I., Burns A. T., Mullinix K. P., Binastein M., Goldberg R. F. Primary activation of the vitellogenin gene in the rooster. J Biol Chem. 1977 Nov 25;252(22):8310–8319. [PubMed] [Google Scholar]
  6. Ding G. J., Lu A. Y., Pickett C. B. Rat liver glutathione S-transferases. Nucleotide sequence analysis of a Yb1 cDNA clone and prediction of the complete amino acid sequence of the Yb1 subunit. J Biol Chem. 1985 Oct 25;260(24):13268–13271. [PubMed] [Google Scholar]
  7. Farber E. Cellular biochemistry of the stepwise development of cancer with chemicals: G. H. A. Clowes memorial lecture. Cancer Res. 1984 Dec;44(12 Pt 1):5463–5474. [PubMed] [Google Scholar]
  8. Feinberg A. P., Vogelstein B. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem. 1983 Jul 1;132(1):6–13. doi: 10.1016/0003-2697(83)90418-9. [DOI] [PubMed] [Google Scholar]
  9. Gubler U., Hoffman B. J. A simple and very efficient method for generating cDNA libraries. Gene. 1983 Nov;25(2-3):263–269. doi: 10.1016/0378-1119(83)90230-5. [DOI] [PubMed] [Google Scholar]
  10. Kitahara A., Satoh K., Nishimura K., Ishikawa T., Ruike K., Sato K., Tsuda H., Ito N. Changes in molecular forms of rat hepatic glutathione S-transferase during chemical hepatocarcinogenesis. Cancer Res. 1984 Jun;44(6):2698–2703. [PubMed] [Google Scholar]
  11. Lai H. C., Grove G., Tu C. P. Cloning and sequence analysis of a cDNA for a rat liver glutathione S-transferase Yb subunit. Nucleic Acids Res. 1986 Aug 11;14(15):6101–6114. doi: 10.1093/nar/14.15.6101. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Lai H. C., Li N., Weiss M. J., Reddy C. C., Tu C. P. The nucleotide sequence of a rat liver glutathione S-transferase subunit cDNA clone. J Biol Chem. 1984 May 10;259(9):5536–5542. [PubMed] [Google Scholar]
  13. Mannervik B., Alin P., Guthenberg C., Jensson H., Tahir M. K., Warholm M., Jörnvall H. Identification of three classes of cytosolic glutathione transferase common to several mammalian species: correlation between structural data and enzymatic properties. Proc Natl Acad Sci U S A. 1985 Nov;82(21):7202–7206. doi: 10.1073/pnas.82.21.7202. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Mannervik B. The isoenzymes of glutathione transferase. Adv Enzymol Relat Areas Mol Biol. 1985;57:357–417. doi: 10.1002/9780470123034.ch5. [DOI] [PubMed] [Google Scholar]
  15. Meyer D. J., Beale D., Tan K. H., Coles B., Ketterer B. Glutathione transferases in primary rat hepatomas: the isolation of a form with GSH peroxidase activity. FEBS Lett. 1985 May 6;184(1):139–143. doi: 10.1016/0014-5793(85)80670-0. [DOI] [PubMed] [Google Scholar]
  16. Pemble S. E., Taylor J. B., Craig R. K., Ketterer B. Differential tissue expression of the glutathione transferase multigene family. Biochem J. 1986 Sep 1;238(2):373–378. doi: 10.1042/bj2380373. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Pickett C. B., Telakowski-Hopkins C. A., Ding G. J., Argenbright L., Lu A. Y. Rat liver glutathione S-transferases. Complete nucleotide sequence of a glutathione S-transferase mRNA and the regulation of the Ya, Yb, and Yc mRNAs by 3-methylcholanthrene and phenobarbital. J Biol Chem. 1984 Apr 25;259(8):5182–5188. [PubMed] [Google Scholar]
  18. Rothkopf G. S., Telakowski-Hopkins C. A., Stotish R. L., Pickett C. B. Multiplicity of glutathione S-transferase genes in the rat and association with a type 2 Alu repetitive element. Biochemistry. 1986 Mar 11;25(5):993–1002. doi: 10.1021/bi00353a007. [DOI] [PubMed] [Google Scholar]
  19. Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Satoh K., Kitahara A., Soma Y., Inaba Y., Hatayama I., Sato K. Purification, induction, and distribution of placental glutathione transferase: a new marker enzyme for preneoplastic cells in the rat chemical hepatocarcinogenesis. Proc Natl Acad Sci U S A. 1985 Jun;82(12):3964–3968. doi: 10.1073/pnas.82.12.3964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Soma Y., Satoh K., Sato K. Purification and subunit-structural and immunological characterization of five glutathione S-transferases in human liver, and the acidic form as a hepatic tumor marker. Biochim Biophys Acta. 1986 Feb 14;869(3):247–258. doi: 10.1016/0167-4838(86)90064-6. [DOI] [PubMed] [Google Scholar]
  22. Suguoka Y., Kano T., Okuda A., Sakai M., Kitagawa T., Muramatsu M. Cloning and the nucleotide sequence of rat glutathione S-transferase P cDNA. Nucleic Acids Res. 1985 Sep 11;13(17):6049–6057. doi: 10.1093/nar/13.17.6049. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Taylor J. B., Craig R. K., Beale D., Ketterer B. Construction and characterization of a plasmid containing complementary DNA to mRNA encoding the N-terminal amino acid sequence of the rat glutathione transferase Ya subunit. Biochem J. 1984 Apr 1;219(1):223–231. doi: 10.1042/bj2190223. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Telakowski-Hopkins C. A., Rodkey J. A., Bennett C. D., Lu A. Y., Pickett C. B. Rat liver glutathione S-transferases. Construction of a cDNA clone complementary to a Yc mRNA and prediction of the complete amino acid sequence of a Yc subunit. J Biol Chem. 1985 May 10;260(9):5820–5825. [PubMed] [Google Scholar]
  25. Wood W. I., Gitschier J., Lasky L. A., Lawn R. M. Base composition-independent hybridization in tetramethylammonium chloride: a method for oligonucleotide screening of highly complex gene libraries. Proc Natl Acad Sci U S A. 1985 Mar;82(6):1585–1588. doi: 10.1073/pnas.82.6.1585. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES