Skip to main content
Biochemical Journal logoLink to Biochemical Journal
. 1987 Sep 1;246(2):425–429. doi: 10.1042/bj2460425

Plasma lipoprotein and apolipoprotein distribution as a function of density in the rainbow trout (Salmo gairdneri).

P J Babin 1
PMCID: PMC1148292  PMID: 3689318

Abstract

I have previously described [Babin (1987) J. Biol. Chem. 262, 4290-4296] the apolipoprotein composition of the major classes of trout plasma lipoproteins. The present work describes the use of an isopycnic density gradient centrifugation procedure and sequential flotation ultracentrifugation to show: (1) the presence of intermediate density lipoproteins (IDL) in the plasma, between 1.015 and 1.040 g/ml; (2) the existence of a single type of Mr 240,000 apoB-like in the low density lipoproteins (LDL, 1.040 less than p less than 1.085 g/ml); (3) the presence of apoA-I-like (Mr 25,000) in the densest LDL; (4) the adequacy of 1.085 g/ml as a cutoff between the LDL and high density lipoproteins (HDL); (5) the accumulation of Mr 55,000 and 76,000 apolipoproteins and apoA-like apolipoproteins in the 1.21 g/ml infranatant. The fractionation of trout lipoprotein spectrum thus furnishes the distribution of the different lipoprotein classes and leads to the description of the constituent apolipoproteins, which account for about 36% of circulating plasma proteins in this species.

Full text

PDF
428

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Babin P. J. Apolipoproteins and the association of egg yolk proteins with plasma high density lipoproteins after ovulation and follicular atresia in the rainbow trout (Salmo gairdneri). J Biol Chem. 1987 Mar 25;262(9):4290–4296. [PubMed] [Google Scholar]
  2. Babin P. J. Effect of plasma lipoproteins in gonadotropin stimulation of 17 beta-estradiol production in the ovarian follicle of rainbow trout (Salmo gairdneri). Gen Comp Endocrinol. 1986 Dec;64(3):456–467. doi: 10.1016/0016-6480(86)90083-3. [DOI] [PubMed] [Google Scholar]
  3. Chapman M. J. Animal lipoproteins: chemistry, structure, and comparative aspects. J Lipid Res. 1980 Sep;21(7):789–853. [PubMed] [Google Scholar]
  4. Chapman M. J., Forgez P., Lagrange D., Goldstein S., Mills G. L. Chimpanzee serum lipoproteins. Isolation, characterisation and comparative aspects of the low density lipoprotein and apolipoprotein-BH. Atherosclerosis. 1984 Aug;52(2):129–149. doi: 10.1016/0021-9150(84)90112-6. [DOI] [PubMed] [Google Scholar]
  5. Chapman M. J., Goldstein S., Lagrange D., Laplaud P. M. A density gradient ultracentrifugal procedure for the isolation of the major lipoprotein classes from human serum. J Lipid Res. 1981 Feb;22(2):339–358. [PubMed] [Google Scholar]
  6. Chapman M. J., Goldstein S., Mills G. L., Leger C. Distribution and characterization of the serum lipoproteins and their apoproteins in the rainbow trout (Salmo gairdnerii). Biochemistry. 1978 Oct 17;17(21):4455–4464. doi: 10.1021/bi00614a015. [DOI] [PubMed] [Google Scholar]
  7. Chapman M. J., Mills G. L. Characterization of the serum lipoproteins and their apoproteins in hypercholesterolaemic guinea pigs. Biochem J. 1977 Oct 1;167(1):9–21. doi: 10.1042/bj1670009. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Dannevig B. H., Norum K. R. Cholesterol esterification and lipids in blood plasma of the char (Salmo alpinus L.) during sexual maturation. Comp Biochem Physiol B. 1982;73(4):771–777. doi: 10.1016/0305-0491(82)90316-9. [DOI] [PubMed] [Google Scholar]
  9. Dannevig B. H., Norum K. R. Effects of fasting on plasma lipids and cholesterol esterification in plasma, liver and intestinal mucosa in the char (Salmo alpinus L.). Comp Biochem Physiol B. 1983;74(2):243–250. doi: 10.1016/0305-0491(83)90007-x. [DOI] [PubMed] [Google Scholar]
  10. Fremont L., Marion D. A comparison of the lipoprotein profiles in male trout (Salmo gairdneri) before maturity and during spermiation. Comp Biochem Physiol B. 1982;73(4):849–855. doi: 10.1016/0305-0491(82)90328-5. [DOI] [PubMed] [Google Scholar]
  11. Gotto A. M., Jr, Pownall H. J., Havel R. J. Introduction to the plasma lipoproteins. Methods Enzymol. 1986;128:3–41. doi: 10.1016/0076-6879(86)28061-1. [DOI] [PubMed] [Google Scholar]
  12. Green P. H., Glickman R. M., Riley J. W., Quinet E. Human apolipoprotein A-IV. Intestinal origin and distribution in plasma. J Clin Invest. 1980 Apr;65(4):911–919. doi: 10.1172/JCI109745. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. HAVEL R. J., EDER H. A., BRAGDON J. H. The distribution and chemical composition of ultracentrifugally separated lipoproteins in human serum. J Clin Invest. 1955 Sep;34(9):1345–1353. doi: 10.1172/JCI103182. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Hermier D., Forgez P., Chapman M. J. A density gradient study of the lipoprotein and apolipoprotein distribution in the chicken, Gallus domesticus. Biochim Biophys Acta. 1985 Aug 22;836(1):105–118. doi: 10.1016/0005-2760(85)90226-7. [DOI] [PubMed] [Google Scholar]
  15. Kane J. P. Apolipoprotein B: structural and metabolic heterogeneity. Annu Rev Physiol. 1983;45:637–650. doi: 10.1146/annurev.ph.45.030183.003225. [DOI] [PubMed] [Google Scholar]
  16. Kane J. P., Hardman D. A., Paulus H. E. Heterogeneity of apolipoprotein B: isolation of a new species from human chylomicrons. Proc Natl Acad Sci U S A. 1980 May;77(5):2465–2469. doi: 10.1073/pnas.77.5.2465. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Mahley R. W., Weisgraber K. H. An electrophoretic method for the quantitative isolation of human and swine plasma lipoproteins. Biochemistry. 1974 Apr 23;13(9):1964–1969. doi: 10.1021/bi00706a600. [DOI] [PubMed] [Google Scholar]
  18. Markwell M. A., Haas S. M., Bieber L. L., Tolbert N. E. A modification of the Lowry procedure to simplify protein determination in membrane and lipoprotein samples. Anal Biochem. 1978 Jun 15;87(1):206–210. doi: 10.1016/0003-2697(78)90586-9. [DOI] [PubMed] [Google Scholar]
  19. Nelson G. J., Shore V. G. Characterization of the serum high density lipoprotein and apolipoproteins of pink salmon. J Biol Chem. 1974 Jan 25;249(2):536–542. [PubMed] [Google Scholar]
  20. Norberg B., Haux C. Induction, isolation and a characterization of the lipid content of plasma vitellogenin from two Salmo species: rainbow trout (Salmo gairdneri) and sea trout (Salmo trutta). Comp Biochem Physiol B. 1985;81(4):869–876. doi: 10.1016/0305-0491(85)90081-1. [DOI] [PubMed] [Google Scholar]
  21. Patton S., Crozier G. F., Benzon A. A. Serum lipids and the death of spawning Pacific salmon. Nature. 1970 Feb 21;225(5234):754–755. doi: 10.1038/225754b0. [DOI] [PubMed] [Google Scholar]
  22. Redgrave T. G., Roberts D. C., West C. E. Separation of plasma lipoproteins by density-gradient ultracentrifugation. Anal Biochem. 1975 May 12;65(1-2):42–49. doi: 10.1016/0003-2697(75)90488-1. [DOI] [PubMed] [Google Scholar]
  23. Robinson J. S., Mead J. F. Lipid absorption and deposition in rainbow trout (Salmo gairdnerii). Can J Biochem. 1973 Jul;51(7):1050–1058. doi: 10.1139/o73-137. [DOI] [PubMed] [Google Scholar]
  24. Sire M. F., Lutton C., Vernier J. M. New views on intestinal absorption of lipids in teleostean fishes: an ultrastructural and biochemical study in the rainbow trout. J Lipid Res. 1981 Jan;22(1):81–94. [PubMed] [Google Scholar]
  25. Skinner E. R., Rogie A. The isolation and partial characterization of the serum lipoproteins and apolipoproteins of the rainbow trout. Biochem J. 1978 Aug 1;173(2):507–520. doi: 10.1042/bj1730507. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Skinner E. R., Youssef A. M. The characterization of lipoprotein lipase isolated from the post-heparin plasma of the rainbow trout, Salmo gairdneri Richardson. Biochem J. 1982 Jun 1;203(3):727–734. doi: 10.1042/bj2030727. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Utermann G., Beisiegel U. Apolipoprotein A-IV: a protein occurring in human mesenteric lymph chylomicrons and free in plasma. Isolation and quantification. Eur J Biochem. 1979 Sep;99(2):333–343. doi: 10.1111/j.1432-1033.1979.tb13261.x. [DOI] [PubMed] [Google Scholar]
  28. Vernier J. M., Sire M. F. Is the Golgi apparatus the obligatory final step for lipoprotein secretion by intestinal cells? Tissue Cell. 1986;18(3):447–460. doi: 10.1016/0040-8166(86)90064-9. [DOI] [PubMed] [Google Scholar]
  29. Weinberg R. B., Scanu A. M. Isolation and characterization of human apolipoprotein A-IV from lipoprotein-depleted serum. J Lipid Res. 1983 Jan;24(1):52–59. [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES