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. 1987 Sep 1;246(2):555–557. doi: 10.1042/bj2460555

Thyroid status and adenosine content of adipose tissue.

J J Ohisalo 1, S Stoneham 1, L Keso 1
PMCID: PMC1148310  PMID: 3689321

Abstract

Fat-cells from hypothyroid rats lack the normal lipolytic response to catecholamines. We have suggested that this is due to increased sensitivity to the inhibitory actions of endogenous adenosine. In this paper we present evidence that thyroid hormones increase adipose-tissue adenosine contents and suggest that the increased sensitivity to adenosine in hypothyroidism is due to relief from desensitization by endogenous adenosine.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Armstrong K. J., Stouffer J. E., Van Inwegen R. G., Thompson W. J., Robison G. A. Effects of thyroid hormone deficiency on cyclic adenosine 3':5'-monophosphate and control of lipolysis in fat cells. J Biol Chem. 1974 Jul 10;249(13):4226–4231. [PubMed] [Google Scholar]
  2. Chohan P., Carpenter C., Saggerson E. D. Changes in the anti-lipolytic action and binding to plasma membranes of N6-L-phenylisopropyladenosine in adipocytes from starved and hypothyroid rats. Biochem J. 1984 Oct 1;223(1):53–59. doi: 10.1042/bj2230053. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Fredholm B. B., Hedqvist P. Modulation of neurotransmission by purine nucleotides and nucleosides. Biochem Pharmacol. 1980 Jun 15;29(12):1635–1643. doi: 10.1016/0006-2952(80)90117-3. [DOI] [PubMed] [Google Scholar]
  4. Fredholm B. B., Vernet L. Accumulation and inactivation of adenosine by fat cells from hypothyroid rats. Acta Physiol Scand. 1984 Jun;121(2):155–163. doi: 10.1111/j.1748-1716.1984.tb07442.x. [DOI] [PubMed] [Google Scholar]
  5. Hershfield M. S., Krodich N. M. S-adenosylhomocysteine hydrolase is an adenosine-binding protein: a target for adenosine toxicity. Science. 1978 Nov 17;202(4369):757–760. doi: 10.1126/science.715439. [DOI] [PubMed] [Google Scholar]
  6. Hoffman B. B., Chang H., Dall'Aglio E., Reaven G. M. Desensitization of adenosine receptor-mediated inhibition of lipolysis. The mechanism involves the development of enhanced cyclic adenosine monophosphate accumulation in tolerant adipocytes. J Clin Invest. 1986 Jul;78(1):185–190. doi: 10.1172/JCI112550. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hoffman B. B., Chang H., Farahbakhsh Z., Reaven G. Inhibition of lipolysis by adenosine is potentiated with age. J Clin Invest. 1984 Nov;74(5):1750–1755. doi: 10.1172/JCI111593. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kather H., Bieger W., Michel G., Aktories K., Jakobs K. H. Human fat cell lipolysis is primarily regulated by inhibitory modulators acting through distinct mechanisms. J Clin Invest. 1985 Oct;76(4):1559–1565. doi: 10.1172/JCI112137. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kather H., Wieland E., Fischer B., Schlierf G. Antilipolytic effects of N6-phenylisopropyladenosine and prostaglandin E2 in fat-cells of obese volunteers before and during energy restriction. Biochem J. 1985 Nov 1;231(3):531–535. doi: 10.1042/bj2310531. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Malbon C. C., Graziano M. P. Adenosine deaminase normalizes cyclic AMP responses of hypothyroid rat fat cells to forskolin, but not beta-adrenergic agonists. FEBS Lett. 1983 May 2;155(1):35–38. doi: 10.1016/0014-5793(83)80203-8. [DOI] [PubMed] [Google Scholar]
  11. Malbon C. C., Moreno F. J., Cabelli R. J., Fain J. N. Fat cell adenylate cyclase and beta-adrenergic receptors in altered thyroid states. J Biol Chem. 1978 Feb 10;253(3):671–678. [PubMed] [Google Scholar]
  12. Malbon C. C., Rapiejko P. J., Mangano T. J. Fat cell adenylate cyclase system. Enhanced inhibition by adenosine and GTP in the hypothyroid rat. J Biol Chem. 1985 Feb 25;260(4):2558–2564. [PubMed] [Google Scholar]
  13. Mills I., García-Sainz J. A., Fain J. N. Pertussis toxin effects on adenylate cyclase activity, cyclic AMP accumulation and lipolysis in adipocytes from hypothyroid, euthyroid and hyperthyroid rats. Biochim Biophys Acta. 1986 May 21;876(3):619–630. doi: 10.1016/0005-2760(86)90051-2. [DOI] [PubMed] [Google Scholar]
  14. Ohisalo J. J. Modulation of lipolysis by adenosine and Ca2+ in fat cells from hypothyroid rats. FEBS Lett. 1980 Jul 11;116(1):91–94. doi: 10.1016/0014-5793(80)80535-7. [DOI] [PubMed] [Google Scholar]
  15. Ohisalo J. J., Ranta S., Huhtaniemi I. T. Attenuated adenosine R-site effect in adipocytes in obesity. Metabolism. 1986 Feb;35(2):143–146. doi: 10.1016/0026-0495(86)90115-0. [DOI] [PubMed] [Google Scholar]
  16. Ohisalo J. J., Ranta S., Huhtaniemi I. T. Inhibition of adenosine 3',5'-monophosphate accumulation and lipolysis by adenosine analogs in human subcutaneous adipocytes. J Clin Endocrinol Metab. 1984 Jan;58(1):32–35. doi: 10.1210/jcem-58-1-32. [DOI] [PubMed] [Google Scholar]
  17. Ohisalo J. J., Stouffer J. E. Adenosine, thyroid status and regulation of lipolysis. Biochem J. 1979 Jan 15;178(1):249–251. doi: 10.1042/bj1780249. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Ohisalo J. J., Strandberg H., Kostiainen E., Kuusi T., Ehnholm C. Stimulation of lipoprotein lipase activity of rat adipose tissue and post-heparin plasma by N6-(phenylisopropyl)adenosine. FEBS Lett. 1981 Sep 14;132(1):121–123. doi: 10.1016/0014-5793(81)80442-5. [DOI] [PubMed] [Google Scholar]
  19. Parsons W. J., Stiles G. L. Heterologous desensitization of the inhibitory A1 adenosine receptor-adenylate cyclase system in rat adipocytes. Regulation of both Ns and Ni. J Biol Chem. 1987 Jan 15;262(2):841–847. [PubMed] [Google Scholar]
  20. RODBELL M. METABOLISM OF ISOLATED FAT CELLS. I. EFFECTS OF HORMONES ON GLUCOSE METABOLISM AND LIPOLYSIS. J Biol Chem. 1964 Feb;239:375–380. [PubMed] [Google Scholar]
  21. Ranta S., Kiviluoto T., Newby A. C., Ohisalo J. J. Assay of adenosine in human adipose tissue. Acta Endocrinol (Copenh) 1985 Nov;110(3):429–432. doi: 10.1530/acta.0.1100429. [DOI] [PubMed] [Google Scholar]
  22. Rapiejko P. J., Malbon C. C. Short-term hyperthyroidism modulates adenosine receptors and catalytic activity of adenylate cyclase in adipocytes. Biochem J. 1987 Feb 1;241(3):765–771. doi: 10.1042/bj2410765. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Saggerson E. D. Increased antilipolytic effect of the adenosine 'R-site' agonist N6-(phenylisopropyl)adenosine in adipocytes from adrenalectomized rats. FEBS Lett. 1980 Jun 16;115(1):127–128. doi: 10.1016/0014-5793(80)80741-1. [DOI] [PubMed] [Google Scholar]
  24. Saggerson E. D. Sensitivity of adipocyte lipolysis to stimulatory and inhibitory agonists in hypothyroidism and starvation. Biochem J. 1986 Sep 1;238(2):387–394. doi: 10.1042/bj2380387. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Schrader J., Schütz W., Bardenheuer H. Role of S-adenosylhomocysteine hydrolase in adenosine metabolism in mammalian heart. Biochem J. 1981 Apr 15;196(1):65–70. doi: 10.1042/bj1960065. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Schwabe U., Schönhöfer P. S., Ebert R. Facilitation by adenosine of the action of insulin on the accumulation of adenosine 3':5'-monophosphate, lipolysis, and glucose oxidation in isolated fat cells. Eur J Biochem. 1974 Aug 1;46(3):537–545. doi: 10.1111/j.1432-1033.1974.tb03647.x. [DOI] [PubMed] [Google Scholar]
  27. Vernon R. G., Finley E. Regulation of lipolysis during pregnancy and lactation in sheep. Response to noradrenaline and adenosine. Biochem J. 1985 Sep 15;230(3):651–656. doi: 10.1042/bj2300651. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Woodward J. A., Saggerson E. D. Effect of adenosine deaminase, N6-phenylisopropyladenosine and hypothyroidism on the responsiveness of rat brown adipocytes to noradrenaline. Biochem J. 1986 Sep 1;238(2):395–403. doi: 10.1042/bj2380395. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Worku Y., Newby A. C. The mechanism of adenosine production in rat polymorphonuclear leucocytes. Biochem J. 1983 Aug 15;214(2):325–330. doi: 10.1042/bj2140325. [DOI] [PMC free article] [PubMed] [Google Scholar]

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