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. 1987 Oct 15;247(2):293–297. doi: 10.1042/bj2470293

Light-induced changes in the content of inositol phosphates in squid (Loligo pealei) retina.

J E Brown 1, D C Watkins 1, C C Malbon 1
PMCID: PMC1148407  PMID: 2827625

Abstract

Illumination induced an increase in inositol polyphosphates, inositol 1,4-bisphosphate and inositol 1,4,5-trisphosphate, in the photoreceptors of the squid, Loligo pealei. There was a concomitant decrease in phosphatidylinositol 4,5-bisphosphate, but no light-induced change in any other phosphoinositide. None of these stimulus-induced changes were altered by treatment in vivo with pertussis toxin, which ADP-ribosylated a Mr-39000-peptide. These findings support the hypothesis that inositol 1,4,5-trisphosphate participates as either a messenger or a modulator in transduction in invertebrate photoreceptors.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bentrop J., Paulsen R. Light-modulated ADP-ribosylation, protein phosphorylation and protein binding in isolated fly photoreceptor membranes. Eur J Biochem. 1986 Nov 17;161(1):61–67. doi: 10.1111/j.1432-1033.1986.tb10124.x. [DOI] [PubMed] [Google Scholar]
  2. Bolsover S. R., Brown J. E. Injection of guanosine and adenosine nucleotides into Limulus ventral photoreceptor cells. J Physiol. 1982 Nov;332:325–342. doi: 10.1113/jphysiol.1982.sp014416. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Brown J. E., Rubin L. J. A direct demonstration that inositol-trisphosphate induces an increase in intracellular calcium in Limulus photoreceptors. Biochem Biophys Res Commun. 1984 Dec 28;125(3):1137–1142. doi: 10.1016/0006-291x(84)91402-5. [DOI] [PubMed] [Google Scholar]
  4. Brown J. E., Rubin L. J., Ghalayini A. J., Tarver A. P., Irvine R. F., Berridge M. J., Anderson R. E. myo-Inositol polyphosphate may be a messenger for visual excitation in Limulus photoreceptors. Nature. 1984 Sep 13;311(5982):160–163. doi: 10.1038/311160a0. [DOI] [PubMed] [Google Scholar]
  5. Calhoon R., Tsuda M., Ebrey T. G. A light-activated GTPase from octopus photoreceptors. Biochem Biophys Res Commun. 1980 Jun 30;94(4):1452–1457. doi: 10.1016/0006-291x(80)90582-3. [DOI] [PubMed] [Google Scholar]
  6. Cohen A. I. An ultrastructural analysis of the photoreceptors of the squid and their synaptic connections. I. Photoreceptive and non-synaptic regions of the retina. J Comp Neurol. 1973 Feb 1;147(3):351–378. doi: 10.1002/cne.901470304. [DOI] [PubMed] [Google Scholar]
  7. Downes C. P., Mussat M. C., Michell R. H. The inositol trisphosphate phosphomonoesterase of the human erythrocyte membrane. Biochem J. 1982 Apr 1;203(1):169–177. doi: 10.1042/bj2030169. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Fein A. Blockade of visual excitation and adaptation in Limulus photoreceptor by GDP-beta-S. Science. 1986 Jun 20;232(4757):1543–1545. doi: 10.1126/science.3487116. [DOI] [PubMed] [Google Scholar]
  9. Fein A., Payne R., Corson D. W., Berridge M. J., Irvine R. F. Photoreceptor excitation and adaptation by inositol 1,4,5-trisphosphate. Nature. 1984 Sep 13;311(5982):157–160. doi: 10.1038/311157a0. [DOI] [PubMed] [Google Scholar]
  10. Gilman A. G. G proteins and dual control of adenylate cyclase. Cell. 1984 Mar;36(3):577–579. doi: 10.1016/0092-8674(84)90336-2. [DOI] [PubMed] [Google Scholar]
  11. Gonzalez-Sastre F., Folch-Pi J. Thin-layer chromatography of the phosphoinositides. J Lipid Res. 1968 Jul;9(4):532–533. [PubMed] [Google Scholar]
  12. Irvine R. F., Anggård E. E., Letcher A. J., Downes C. P. Metabolism of inositol 1,4,5-trisphosphate and inositol 1,3,4-trisphosphate in rat parotid glands. Biochem J. 1985 Jul 15;229(2):505–511. doi: 10.1042/bj2290505. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Katada T., Ui M. ADP ribosylation of the specific membrane protein of C6 cells by islet-activating protein associated with modification of adenylate cyclase activity. J Biol Chem. 1982 Jun 25;257(12):7210–7216. [PubMed] [Google Scholar]
  14. Litosch I., Fain J. N. Regulation of phosphoinositide breakdown by guanine nucleotides. Life Sci. 1986 Jul 21;39(3):187–194. doi: 10.1016/0024-3205(86)90529-1. [DOI] [PubMed] [Google Scholar]
  15. Majerus P. W., Connolly T. M., Deckmyn H., Ross T. S., Bross T. E., Ishii H., Bansal V. S., Wilson D. B. The metabolism of phosphoinositide-derived messenger molecules. Science. 1986 Dec 19;234(4783):1519–1526. doi: 10.1126/science.3024320. [DOI] [PubMed] [Google Scholar]
  16. Malbon C. C., Kaupp U. B., Brown J. E. Limulus ventral photoreceptors contain a homologue of the alpha-subunit of mammalian Ns. FEBS Lett. 1984 Jun 25;172(1):91–94. doi: 10.1016/0014-5793(84)80880-7. [DOI] [PubMed] [Google Scholar]
  17. Malbon C. C., Rapiejko P. J., Mangano T. J. Fat cell adenylate cyclase system. Enhanced inhibition by adenosine and GTP in the hypothyroid rat. J Biol Chem. 1985 Feb 25;260(4):2558–2564. [PubMed] [Google Scholar]
  18. Payne R., Corson D. W., Fein A., Berridge M. J. Excitation and adaptation of Limulus ventral photoreceptors by inositol 1,4,5 triphosphate result from a rise in intracellular calcium. J Gen Physiol. 1986 Jul;88(1):127–142. doi: 10.1085/jgp.88.1.127. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Rapiejko P. J., Northup J. K., Evans T., Brown J. E., Malbon C. C. G-proteins of fat-cells. Role in hormonal regulation of intracellular inositol 1,4,5-trisphosphate. Biochem J. 1986 Nov 15;240(1):35–40. doi: 10.1042/bj2400035. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Saibil H. R., Michel-Villaz M. Squid rhodopsin and GTP-binding protein crossreact with vertebrate photoreceptor enzymes. Proc Natl Acad Sci U S A. 1984 Aug;81(16):5111–5115. doi: 10.1073/pnas.81.16.5111. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Stryer L., Bourne H. R. G proteins: a family of signal transducers. Annu Rev Cell Biol. 1986;2:391–419. doi: 10.1146/annurev.cb.02.110186.002135. [DOI] [PubMed] [Google Scholar]
  22. Szuts E. Z., Wood S. F., Reid M. S., Fein A. Light stimulates the rapid formation of inositol trisphosphate in squid retinas. Biochem J. 1986 Dec 15;240(3):929–932. doi: 10.1042/bj2400929. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Vandenberg C. A., Montal M. Light-regulated biochemical events in invertebrate photoreceptors. 2. Light-regulated phosphorylation of rhodopsin and phosphoinositides in squid photoreceptor membranes. Biochemistry. 1984 May 22;23(11):2347–2352. doi: 10.1021/bi00306a004. [DOI] [PubMed] [Google Scholar]
  24. Yoshioka T., Inoue H., Takagi M., Hayashi F., Amakawa T. The effect of isobutylmethylxanthine on the photoresponse and phosphorylation of phosphatidylinositol in octopus retina. Biochim Biophys Acta. 1983 Jan 4;755(1):50–55. doi: 10.1016/0304-4165(83)90271-4. [DOI] [PubMed] [Google Scholar]

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