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. 1988 Jan 15;249(2):319–326. doi: 10.1042/bj2490319

Sequencing and overexpression of the Escherichia coli aroE gene encoding shikimate dehydrogenase.

I A Anton 1, J R Coggins 1
PMCID: PMC1148705  PMID: 3277621

Abstract

The Escherichia coli aroE gene encoding shikimate dehydrogenase was sequenced. The deduced amino acid sequence was confirmed by N-terminal amino acid sequencing and amino acid analysis of the overproduced protein. The complete polypeptide chain has 272 amino acid residues and has a calculated Mr of 29,380. E. coli shikimate dehydrogenase is homologous to the shikimate dehydrogenase domain of the fungal arom multifunctional enzymes and to the catabolic quinate dehydrogenase of Neurospora crassa.

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Selected References

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