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. 1988 Jan 15;249(2):613–616. doi: 10.1042/bj2490613

Nucleotide sequence for the hemD gene of Escherichia coli encoding uroporphyrinogen III synthase and initial evidence for a hem operon.

P M Jordan 1, B I Mgbeje 1, S D Thomas 1, A F Alwan 1
PMCID: PMC1148746  PMID: 3277628

Abstract

1. The hemD gene, encoding uroporphyrinogen III synthase, has been located adjacent to the hemC gene at 85 min on the Escherichia coli chromosome. 2. The entire nucleotide sequence (741 base pairs) of the hemD gene is reported. 3. E. coli strains harbouring plasmics containing the hemD gene produce greatly elevated levels of uroporphyrinogen III synthase. 4. Purified uroporphyrinogen III synthase, isolated from the hemD-containing strain ST1046, has an Mr of 29,000, in close agreement with that predicted from the nucleotide sequence. 5. The existence of a hem operon is suggested.

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Selected References

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  1. Amann E., Brosius J. "ATG vectors' for regulated high-level expression of cloned genes in Escherichia coli. Gene. 1985;40(2-3):183–190. doi: 10.1016/0378-1119(85)90041-1. [DOI] [PubMed] [Google Scholar]
  2. Bachmann B. J. Linkage map of Escherichia coli K-12, edition 7. Microbiol Rev. 1983 Jun;47(2):180–230. doi: 10.1128/mr.47.2.180-230.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Boyer H. W., Roulland-Dussoix D. A complementation analysis of the restriction and modification of DNA in Escherichia coli. J Mol Biol. 1969 May 14;41(3):459–472. doi: 10.1016/0022-2836(69)90288-5. [DOI] [PubMed] [Google Scholar]
  4. Hart G. J., Battersby A. R. Purification and properties of uroporphyrinogen III synthase (co-synthetase) from Euglena gracilis. Biochem J. 1985 Nov 15;232(1):151–160. doi: 10.1042/bj2320151. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Jacobs N. J., Jacobs J. M. Microbial oxidation of protoporhydrinogen, an intermediate in heme and chlorphyll biosynthesis. Arch Biochem Biophys. 1979 Oct 15;197(2):396–403. doi: 10.1016/0003-9861(79)90261-3. [DOI] [PubMed] [Google Scholar]
  6. Jordan P. M., Berry A. Preuroporphyrinogen, a universal intermediate in the biosynthesis of uroporphyrinogen III. FEBS Lett. 1980 Mar 24;112(1):86–88. doi: 10.1016/0014-5793(80)80134-7. [DOI] [PubMed] [Google Scholar]
  7. Jordan P. M., Shemin D. Purification and properties of uroporphyrinogen I synthetase from Rhodopseudomonas spheroides. J Biol Chem. 1973 Feb 10;248(3):1019–1024. [PubMed] [Google Scholar]
  8. Jordan P. M. Uroporphyrinogen III cosynthetase: a direct assay method. Enzyme. 1982;28(2-3):158–169. doi: 10.1159/000459099. [DOI] [PubMed] [Google Scholar]
  9. McConville M. L., Charles H. P. Mutants of Escherichia coli K12 accumulating porphobilinogen: a new locus, hemC. J Gen Microbiol. 1979 Mar;111(1):193–200. doi: 10.1099/00221287-111-1-193. [DOI] [PubMed] [Google Scholar]
  10. Messing J. New M13 vectors for cloning. Methods Enzymol. 1983;101:20–78. doi: 10.1016/0076-6879(83)01005-8. [DOI] [PubMed] [Google Scholar]
  11. Piggot P. J., Hoch J. A. Revised genetic linkage map of Bacillus subtilis. Microbiol Rev. 1985 Jun;49(2):158–179. doi: 10.1128/mr.49.2.158-179.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Staden R., McLachlan A. D. Codon preference and its use in identifying protein coding regions in long DNA sequences. Nucleic Acids Res. 1982 Jan 11;10(1):141–156. doi: 10.1093/nar/10.1.141. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Thomas S. D., Jordan P. M. Nucleotide sequence of the hemC locus encoding porphobilinogen deaminase of Escherichia coli K12. Nucleic Acids Res. 1986 Aug 11;14(15):6215–6226. doi: 10.1093/nar/14.15.6215. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Tien W., White D. C. Linear sequential arrangement of genes for the biosynthetic pathway of protoheme in Staphylococcus aureus. Proc Natl Acad Sci U S A. 1968 Dec;61(4):1392–1398. doi: 10.1073/pnas.61.4.1392. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Tsai S. F., Bishop D. F., Desnick R. J. Purification and properties of uroporphyrinogen III synthase from human erythrocytes. J Biol Chem. 1987 Jan 25;262(3):1268–1273. [PubMed] [Google Scholar]

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