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. 1988 Feb 1;249(3):807–811. doi: 10.1042/bj2490807

Adenylate cyclase activity in a higher plant, alfalfa (Medicago sativa).

V C Carricarte 1, G M Bianchini 1, J P Muschietti 1, M T Téllez-Iñón 1, A Perticari 1, N Torres 1, M M Flawiá 1
PMCID: PMC1148778  PMID: 3128270

Abstract

An adenylate cyclase activity in Medicago sativa L. (alfalfa) roots was partially characterized. The enzyme activity remains in the supernatant fluid after centrifugation at 105,000 g and shows in crude extracts an apparent Mr of about 84,000. The enzyme is active with Mg2+ and Ca2+ as bivalent cations, and is inhibited by EGTA and by chlorpromazine. Calmodulin from bovine brain or spinach leaves activates this adenylate cyclase.

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Selected References

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