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. 1988 May 1;251(3):931–933. doi: 10.1042/bj2510931

Expression of human liver epoxide hydrolase in Saccharomyces pombe.

M R Jackson 1, B Burchell 1
PMCID: PMC1149092  PMID: 3415653

Abstract

Human liver microsomal epoxide hydrolase cDNA was inserted into the yeast expression vector pEVP11. The resulting recombinant plasmid was introduced into Saccharomyces pombe. The epoxide hydrolase protein and enzymic activity was subsequently expressed and identified in the 105,000 g pellet after centrifugal fractionation of homogenized yeast cells. This method will provide a useful source of human liver epoxide hydrolase, avoiding the problems of obtaining human tissue.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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