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. 1988 May 15;252(1):39–45. doi: 10.1042/bj2520039

Plant holo-(acyl carrier protein) synthase.

S A Elhussein 1, J A Miernyk 1, J B Ohlrogge 1
PMCID: PMC1149104  PMID: 2844150

Abstract

1. An improved method was developed for the assay of plant holo-(acyl carrier protein) synthase activity, using Escherichia coli acyl-(acyl carrier protein) synthetase as a coupling enzyme. 2. Holo-(acyl carrier protein) synthase was partially purified from spinach (Spinacia oleracea) leaves by a combination of (NH4)2SO4 fractionation and anion-exchange and gel-permeation chromatography. 3. The partially purified enzyme had a pH optimum of 8.2 and Km values of 2 microM, 72 microM and 3 mM for apo-(acyl carrier protein), CoA and Mg2+ respectively. Synthase activity was inhibited in vitro by the reaction product 3',5'-ADP. 4. Results from the fractionation of spinach leaf and developing castor-oil-seed (Ricinus communis) endosperm cells were consistent with a cytosolic localization of holo-(acyl carrier protein) synthase activity in plant cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alberts A. W., Vagelos P. R. Acyl carrier protein. 8. Studies of acyl carrier protein and coenzyme A in Escherichia coli pantothenate or betaalanine auxotrophs. J Biol Chem. 1966 Nov 25;241(22):5201–5204. [PubMed] [Google Scholar]
  2. Carrillo N. Biosynthesis of ferredoxin-NADP+ oxidoreductase. Evidence for the formation of a functional preholoenzyme in the cytoplasmic compartment. Eur J Biochem. 1985 Aug 1;150(3):469–474. doi: 10.1111/j.1432-1033.1985.tb09045.x. [DOI] [PubMed] [Google Scholar]
  3. Elovson J., Vagelos P. R. Acyl carrier protein. X. Acyl carrier protein synthetase. J Biol Chem. 1968 Jul 10;243(13):3603–3611. [PubMed] [Google Scholar]
  4. Enosawa S., Ohashi A. Localization of enzyme for heme attachment to apocytochrome c in yeast mitochondria. Biochem Biophys Res Commun. 1986 Dec 30;141(3):1145–1150. doi: 10.1016/s0006-291x(86)80163-2. [DOI] [PubMed] [Google Scholar]
  5. Garland W. J., Dennis D. T. Steady-state kinetics of glutamate dehydrogenase from Pisum sativum L. mitochondria. Arch Biochem Biophys. 1977 Aug;182(2):614–625. doi: 10.1016/0003-9861(77)90542-2. [DOI] [PubMed] [Google Scholar]
  6. Guerra D. J., Ohlrogge J. B. Partial purification and characterization of two forms of malonyl-coenzyme A:acyl carrier protein transacylase from soybean leaf tissue. Arch Biochem Biophys. 1986 Apr;246(1):274–285. doi: 10.1016/0003-9861(86)90473-x. [DOI] [PubMed] [Google Scholar]
  7. Harbin B. M., Dailey H. A. Orientation of ferrochelatase in bovine liver mitochondria. Biochemistry. 1985 Jan 15;24(2):366–370. doi: 10.1021/bi00323a019. [DOI] [PubMed] [Google Scholar]
  8. Jackowski S., Rock C. O. Turnover of the 4'-phosphopantetheine prosthetic group of acyl carrier protein. J Biol Chem. 1984 Feb 10;259(3):1891–1895. [PubMed] [Google Scholar]
  9. Kuo T. M., Ohlrogge J. B. Acylation of plant acyl carrier proteins by acyl-acyl carrier protein synthetase from Escherichia coli. Arch Biochem Biophys. 1984 Apr;230(1):110–116. doi: 10.1016/0003-9861(84)90091-2. [DOI] [PubMed] [Google Scholar]
  10. Lazarow P. B., de Duve C. The synthesis and turnover of rat liver peroxisomes. V. Intracellular pathway of catalase synthesis. J Cell Biol. 1973 Nov;59(2 Pt 1):507–524. doi: 10.1083/jcb.59.2.507. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Ohlrogge J. B., Kuhn D. N., Stumpf P. K. Subcellular localization of acyl carrier protein in leaf protoplasts of Spinacia oleracea. Proc Natl Acad Sci U S A. 1979 Mar;76(3):1194–1198. doi: 10.1073/pnas.76.3.1194. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Polacco M. L., Cronan J. E., Jr A mutant of Escherichia coli conditionally defective in the synthesis of holo-[acyl carrier protein]. J Biol Chem. 1981 Jun 10;256(11):5750–5754. [PubMed] [Google Scholar]
  13. Powell G. L., Elovson J., Vagelos P. R. Acyl carrier protein. XII. Synthesis and turnover of the prosthetic group of acyl carrier protein in vivo. J Biol Chem. 1969 Oct 25;244(20):5616–5624. [PubMed] [Google Scholar]
  14. Prescott D. J., Elovson J., Vagelos P. R. Acyl carrier protein. XI. The specificity of acyl carrier protein synthetase. J Biol Chem. 1969 Aug 25;244(16):4517–4521. [PubMed] [Google Scholar]
  15. Read S. M., Northcote D. H. Minimization of variation in the response to different proteins of the Coomassie blue G dye-binding assay for protein. Anal Biochem. 1981 Sep 1;116(1):53–64. doi: 10.1016/0003-2697(81)90321-3. [DOI] [PubMed] [Google Scholar]
  16. Rock C. O., Cronan J. E., Jr Improved purification of acyl carrier protein. Anal Biochem. 1980 Mar 1;102(2):362–364. doi: 10.1016/0003-2697(80)90168-2. [DOI] [PubMed] [Google Scholar]
  17. Schmidt G. W., Mishkind M. L. The transport of proteins into chloroplasts. Annu Rev Biochem. 1986;55:879–912. doi: 10.1146/annurev.bi.55.070186.004311. [DOI] [PubMed] [Google Scholar]
  18. Sharma C. P., Gehring H. The precursor of mitochondrial aspartate aminotransferase is translocated into mitochondria as apoprotein. J Biol Chem. 1986 Aug 25;261(24):11146–11149. [PubMed] [Google Scholar]
  19. Takahashi Y., Mitsui A., Hase T., Matsubara H. Formation of the iron-sulfur cluster of ferredoxin in isolated chloroplasts. Proc Natl Acad Sci U S A. 1986 Apr;83(8):2434–2437. doi: 10.1073/pnas.83.8.2434. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Vagelos P. R., Larrabes A. R. Acyl carrier protein. IX. Acyl carrier protein hydrolase. J Biol Chem. 1967 Apr 25;242(8):1776–1781. [PubMed] [Google Scholar]

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