Abstract
Patatin is a family of glycoproteins that accounts for 30-40% of the total soluble protein in potato (Solanum tuberosum) tubers. This protein has been reported not only to serve as a storage protein, but also to exhibit enzymic activity. By using a baculovirus system to express protein from the patatin cDNA clone pGM01, it was unambiguously shown that the patatin coded by this DNA has lipid acyl hydrolase and acyltransferase activities. The enzyme is active with phospholipids, monoacylglycerols and p-nitrophenyl esters, moderately active with galactolipids, but is apparently inactive with di- and tri-acylglycerols.
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- Bostock R. M., Kuc J. A., Laine R. A. Eicosapentaenoic and Arachidonic Acids from Phytophthora infestans Elicit Fungitoxic Sesquiterpenes in the Potato. Science. 1981 Apr 3;212(4490):67–69. doi: 10.1126/science.212.4490.67. [DOI] [PubMed] [Google Scholar]
- Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. doi: 10.1016/0003-2697(76)90527-3. [DOI] [PubMed] [Google Scholar]
- Estes M. K., Crawford S. E., Penaranda M. E., Petrie B. L., Burns J. W., Chan W. K., Ericson B., Smith G. E., Summers M. D. Synthesis and immunogenicity of the rotavirus major capsid antigen using a baculovirus expression system. J Virol. 1987 May;61(5):1488–1494. doi: 10.1128/jvi.61.5.1488-1494.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Galliard T., Matthew J. A. Lipids of potato tubers. II. Lipid-degrading enzymes in different varieties of potato tuber. J Sci Food Agric. 1973 May;24(5):623–627. doi: 10.1002/jsfa.2740240516. [DOI] [PubMed] [Google Scholar]
- Galliard T. The enzymic deacylation of phospholipids and galactolipids in plants. Purification and properties of a lipolytic acyl-hydrolase from potato tubers. Biochem J. 1971 Feb;121(3):379–390. doi: 10.1042/bj1210379. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hannapel D. J., Miller J. C., Park W. D. Regulation of potato tuber protein accumulation by gibberellic Acid. Plant Physiol. 1985 Aug;78(4):700–703. doi: 10.1104/pp.78.4.700. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hasson E. P., Laties G. G. Separation and characterization of potato lipid acylhydrolases. Plant Physiol. 1976 Feb;57(2):142–147. doi: 10.1104/pp.57.2.142. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hink W. F. Established insect cell line from the cabbage looper, Trichoplusia ni. Nature. 1970 May 2;226(5244):466–467. doi: 10.1038/226466b0. [DOI] [PubMed] [Google Scholar]
- Hirayama O., Matsuda H., Takeda H., Maenaka K., Takatsuka H. Purification and properties of a lipid acyl-hydrolase from potato tubers. Biochim Biophys Acta. 1975 Mar 28;384(1):127–137. doi: 10.1016/0005-2744(75)90102-3. [DOI] [PubMed] [Google Scholar]
- Jeang K. T., Giam C. Z., Nerenberg M., Khoury G. Abundant synthesis of functional human T-cell leukemia virus type I p40x protein in eucaryotic cells by using a baculovirus expression vector. J Virol. 1987 Mar;61(3):708–713. doi: 10.1128/jvi.61.3.708-713.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Knudsen J., Hansen H. O., Mikkelsen J. M. Medium-chain fatty acid synthesis. Biochem Soc Trans. 1986 Jun;14(3):575–576. doi: 10.1042/bst0140575. [DOI] [PubMed] [Google Scholar]
- Kuroda K., Hauser C., Rott R., Klenk H. D., Doerfler W. Expression of the influenza virus haemagglutinin in insect cells by a baculovirus vector. EMBO J. 1986 Jun;5(6):1359–1365. doi: 10.1002/j.1460-2075.1986.tb04367.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
- Maeda S., Kawai T., Obinata M., Fujiwara H., Horiuchi T., Saeki Y., Sato Y., Furusawa M. Production of human alpha-interferon in silkworm using a baculovirus vector. Nature. 1985 Jun 13;315(6020):592–594. doi: 10.1038/315592a0. [DOI] [PubMed] [Google Scholar]
- Messing J. New M13 vectors for cloning. Methods Enzymol. 1983;101:20–78. doi: 10.1016/0076-6879(83)01005-8. [DOI] [PubMed] [Google Scholar]
- Mignery G. A., Pikaard C. S., Hannapel D. J., Park W. D. Isolation and sequence analysis of cDNAs for the major potato tuber protein, patatin. Nucleic Acids Res. 1984 Nov 12;12(21):7987–8000. doi: 10.1093/nar/12.21.7987. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Miyamoto C., Smith G. E., Farrell-Towt J., Chizzonite R., Summers M. D., Ju G. Production of human c-myc protein in insect cells infected with a baculovirus expression vector. Mol Cell Biol. 1985 Oct;5(10):2860–2865. doi: 10.1128/mcb.5.10.2860. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Paiva E., Lister R. M., Park W. D. Induction and accumulation of major tuber proteins of potato in stems and petioles. Plant Physiol. 1983 Jan;71(1):161–168. doi: 10.1104/pp.71.1.161. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Park W. D., Blackwood C., Mignery G. A., Hermodson M. A., Lister R. M. Analysis of the Heterogeneity of the 40,000 Molecular Weight Tuber Glycoprotein of Potatoes by Immunological Methods and by NH(2)-Terminal Sequence Analysis. Plant Physiol. 1983 Jan;71(1):156–160. doi: 10.1104/pp.71.1.156. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pikaard C. S., Brusca J. S., Hannapel D. J., Park W. D. The two classes of genes for the major potato tuber protein, patatin, are differentially expressed in tubers and roots. Nucleic Acids Res. 1987 Mar 11;15(5):1979–1994. doi: 10.1093/nar/15.5.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Possee R. D. Cell-surface expression of influenza virus haemagglutinin in insect cells using a baculovirus vector. Virus Res. 1986 Jul;5(1):43–59. doi: 10.1016/0168-1702(86)90064-x. [DOI] [PubMed] [Google Scholar]
- Smith G. E., Summers M. D., Fraser M. J. Production of human beta interferon in insect cells infected with a baculovirus expression vector. Mol Cell Biol. 1983 Dec;3(12):2156–2165. doi: 10.1128/mcb.3.12.2156. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
- St Angelo C., Smith G. E., Summers M. D., Krug R. M. Two of the three influenza viral polymerase proteins expressed by using baculovirus vectors form a complex in insect cells. J Virol. 1987 Feb;61(2):361–365. doi: 10.1128/jvi.61.2.361-365.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Towbin H., Staehelin T., Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350–4354. doi: 10.1073/pnas.76.9.4350. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Vaughn J. L., Goodwin R. H., Tompkins G. J., McCawley P. The establishment of two cell lines from the insect Spodoptera frugiperda (Lepidoptera; Noctuidae). In Vitro. 1977 Apr;13(4):213–217. doi: 10.1007/BF02615077. [DOI] [PubMed] [Google Scholar]