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. 1988 Jul 15;253(2):611–614. doi: 10.1042/bj2530611

Comparative 113Cd-n.m.r. studies on rabbit 113Cd7-, (Zn1,Cd6)- and partially metal-depleted 113Cd6-metallothionein-2a.

F Vazquez 1, M Vasák 1
PMCID: PMC1149342  PMID: 3178730

Abstract

Rabbit 113Cd7-metallothionein-2a (MT) contains two metal-thiolate clusters of three (cluster B) and four (cluster A) metal ions. The 113Cd-n.m.r. spectrum of 113Cd6-MT, isolated from 113Cd7-MT upon treatment with EDTA, is similar to that of 113Cd7-MT, but the cluster B resonances are lower in intensity, suggesting its co-operative metal depletion. (Zn1,113Cd6)-MT, formed upon addition of the Zn(II) ions to 113Cd6-MT, shows 113Cd-n.m.r. features characteristic of cluster B populations containing both Cd(II) and Zn(II) ions. The overall intensity gain of the mixed cluster B resonances per Cd as to those in 113Cd6- and 113Cd7-MT suggests a stabilization effect of the bound Zn(II) ions upon the previously established intramolecular 113Cd exchange within this cluster.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bernhard W. R., Vasák M., Kägi J. H. Cadmium binding and metal cluster formation in metallothionein: a differential modification study. Biochemistry. 1986 Apr 22;25(8):1975–1980. doi: 10.1021/bi00356a021. [DOI] [PubMed] [Google Scholar]
  2. Braun W., Wagner G., Wörgötter E., Vasák M., Kägi J. H., Wüthrich K. Polypeptide fold in the two metal clusters of metallothionein-2 by nuclear magnetic resonance in solution. J Mol Biol. 1986 Jan 5;187(1):125–129. doi: 10.1016/0022-2836(86)90412-2. [DOI] [PubMed] [Google Scholar]
  3. Bühler R. H., Kägi J. H. Spectroscopic properties of zinc-metallothionein. Experientia Suppl. 1979;34:211–220. doi: 10.1007/978-3-0348-6493-0_14. [DOI] [PubMed] [Google Scholar]
  4. Furey W. F., Robbins A. H., Clancy L. L., Winge D. R., Wang B. C., Stout C. D. Crystal structure of Cd,Zn metallothionein. Science. 1986 Feb 14;231(4739):704–710. doi: 10.1126/science.3945804. [DOI] [PubMed] [Google Scholar]
  5. Kimura M., Otaki N., Imano M. Rabbit liver metallothionein. Tentative amino acid sequence of metallothionein-B. Experientia Suppl. 1979;34:163–168. doi: 10.1007/978-3-0348-6493-0_7. [DOI] [PubMed] [Google Scholar]
  6. Kägi J. H., Himmelhoch S. R., Whanger P. D., Bethune J. L., Vallee B. L. Equine hepatic and renal metallothioneins. Purification, molecular weight, amino acid composition, and metal content. J Biol Chem. 1974 Jun 10;249(11):3537–3542. [PubMed] [Google Scholar]
  7. Li T. Y., Kraker A. J., Shaw C. F., 3rd, Petering D. H. Ligand substitution reactions of metallothioneins with EDTA and apo-carbonic anhydrase. Proc Natl Acad Sci U S A. 1980 Nov;77(11):6334–6338. doi: 10.1073/pnas.77.11.6334. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Nettesheim D. G., Engeseth H. R., Otvos J. D. Products of metal exchange reactions of metallothionein. Biochemistry. 1985 Nov 19;24(24):6744–6751. doi: 10.1021/bi00345a003. [DOI] [PubMed] [Google Scholar]
  9. Nielson K. B., Winge D. R. Order of metal binding in metallothionein. J Biol Chem. 1983 Nov 10;258(21):13063–13069. [PubMed] [Google Scholar]
  10. Otvos J. D., Armitage I. M. Structure of the metal clusters in rabbit liver metallothionein. Proc Natl Acad Sci U S A. 1980 Dec;77(12):7094–7098. doi: 10.1073/pnas.77.12.7094. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Otvos J. D., Engeseth H. R., Nettesheim D. G., Hilt C. R. Interprotein metal exchange reactions of metallothionein. Experientia Suppl. 1987;52:171–178. doi: 10.1007/978-3-0348-6784-9_10. [DOI] [PubMed] [Google Scholar]
  12. Otvos J. D., Engeseth H. R., Wehrli S. Preparation and 113Cd NMR studies of homogeneous reconstituted metallothionein: reaffirmation of the two-cluster arrangement of metals. Biochemistry. 1985 Nov 19;24(24):6735–6740. doi: 10.1021/bi00345a001. [DOI] [PubMed] [Google Scholar]
  13. Vasák M., Hawkes G. E., Nicholson J. K., Sadler P. J. 113Cd NMR studies of reconstituted seven-cadmium metallothionein: evidence for structural flexibility. Biochemistry. 1985 Jan 29;24(3):740–747. doi: 10.1021/bi00324a031. [DOI] [PubMed] [Google Scholar]
  14. Vasák M., Kägi J. H. Metal thiolate clusters in cobalt(II)-metallothionein. Proc Natl Acad Sci U S A. 1981 Nov;78(11):6709–6713. doi: 10.1073/pnas.78.11.6709. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Vasák M., Wörgötter E., Wagner G., Kägi J. H., Wüthrich K. Metal co-ordination in rat liver metallothionein-2 prepared with or without reconstitution of the metal clusters, and comparison with rabbit liver metallothionein-2. J Mol Biol. 1987 Aug 5;196(3):711–719. doi: 10.1016/0022-2836(87)90042-8. [DOI] [PubMed] [Google Scholar]
  16. Wagner G., Frey M. H., Neuhaus D., Wörgötter E., Braun W., Vasak M., Kägi J. H., Wüthrich K. Spatial structure of rabbit liver metallothionein-2 in solution by NMR. Experientia Suppl. 1987;52:149–157. doi: 10.1007/978-3-0348-6784-9_8. [DOI] [PubMed] [Google Scholar]
  17. Winge D. R., Miklossy K. A. Domain nature of metallothionein. J Biol Chem. 1982 Apr 10;257(7):3471–3476. [PubMed] [Google Scholar]

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