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. 1991 Jan 15;273(Pt 2):317–321. doi: 10.1042/bj2730317

Inositol 1,3,4,6-tetrakisphosphate mobilizes calcium in Xenopus oocytes with high potency.

I Ivorra 1, R Gigg 1, R F Irvine 1, I Parker 1
PMCID: PMC1149848  PMID: 1991032

Abstract

Injection of Ins(1,3,4,6)P4 into Xenopus oocytes evoked Ca2(+)-dependent membrane currents with a potency 5-10 times less than Ins(1,4,5)P3, whereas Ins(1,3,4)P3 and Ins(1,3,4,5,6)P5 were almost ineffective. Responses to Ins(1,3,4,6)P4 arose through liberation of intracellular Ca2+ and through entry of extracellular Ca2+. These results, together with the observation that Ins(1,3,4,6)P4 facilitated responses to Ins(1,4,5)P3, suggests that both of these compounds may act on the same intracellular receptors.

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Selected References

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  1. Berridge M. J. Inositol trisphosphate-induced membrane potential oscillations in Xenopus oocytes. J Physiol. 1988 Sep;403:589–599. doi: 10.1113/jphysiol.1988.sp017266. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Berridge M. J., Irvine R. F. Inositol phosphates and cell signalling. Nature. 1989 Sep 21;341(6239):197–205. doi: 10.1038/341197a0. [DOI] [PubMed] [Google Scholar]
  3. Challiss R. A., Chilvers E. R., Willcocks A. L., Nahorski S. R. Heterogeneity of [3H]inositol 1,4,5-trisphosphate binding sites in adrenal-cortical membranes. Characterization and validation of a radioreceptor assay. Biochem J. 1990 Jan 15;265(2):421–427. doi: 10.1042/bj2650421. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Clarke N. G., Dawson R. M. Alkaline O leads to N-transacylation. A new method for the quantitative deacylation of phospholipids. Biochem J. 1981 Apr 1;195(1):301–306. doi: 10.1042/bj1950301. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Dawson R. M., Clarke N. D-myoinositol 1:2-cyclic phosphate 2-phosphohydrolase. Biochem J. 1972 Mar;127(1):113–118. doi: 10.1042/bj1270113. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Irvine R. F., Letcher A. J., Lander D. J., Berridge M. J. Specificity of inositol phosphate-stimulated Ca2+ mobilization from Swiss-mouse 3T3 cells. Biochem J. 1986 Nov 15;240(1):301–304. doi: 10.1042/bj2400301. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Irvine R. F., Moor R. M., Pollock W. K., Smith P. M., Wreggett K. A. Inositol phosphates: proliferation, metabolism and function. Philos Trans R Soc Lond B Biol Sci. 1988 Jul 26;320(1199):281–298. doi: 10.1098/rstb.1988.0077. [DOI] [PubMed] [Google Scholar]
  8. Kusano K., Miledi R., Stinnakre J. Cholinergic and catecholaminergic receptors in the Xenopus oocyte membrane. J Physiol. 1982 Jul;328:143–170. doi: 10.1113/jphysiol.1982.sp014257. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Miledi R., Parker I. Chloride current induced by injection of calcium into Xenopus oocytes. J Physiol. 1984 Dec;357:173–183. doi: 10.1113/jphysiol.1984.sp015495. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Nogimori K., Menniti F. S., Putney J. W., Jr Identification in extracts from AR4-2J cells of inositol 1,4,5-trisphosphate by its susceptibility to inositol 1,4,5-trisphosphate 3-kinase and 5-phosphatase. Biochem J. 1990 Jul 1;269(1):195–200. doi: 10.1042/bj2690195. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Oron Y., Dascal N., Nadler E., Lupu M. Inositol 1,4,5-trisphosphate mimics muscarinic response in Xenopus oocytes. Nature. 1985 Jan 10;313(5998):141–143. doi: 10.1038/313141a0. [DOI] [PubMed] [Google Scholar]
  12. Parker I., Gundersen C. B., Miledi R. A transient inward current elicited by hyperpolarization during serotonin activation in Xenopus oocytes. Proc R Soc Lond B Biol Sci. 1985 Jan 22;223(1232):279–292. doi: 10.1098/rspb.1985.0002. [DOI] [PubMed] [Google Scholar]
  13. Parker I., Miledi R. Changes in intracellular calcium and in membrane currents evoked by injection of inositol trisphosphate into Xenopus oocytes. Proc R Soc Lond B Biol Sci. 1986 Aug 22;228(1252):307–315. doi: 10.1098/rspb.1986.0057. [DOI] [PubMed] [Google Scholar]
  14. Parker I., Miledi R. Injection of inositol 1,3,4,5-tetrakisphosphate into Xenopus oocytes generates a chloride current dependent upon intracellular calcium. Proc R Soc Lond B Biol Sci. 1987 Oct 22;232(1266):59–70. doi: 10.1098/rspb.1987.0061. [DOI] [PubMed] [Google Scholar]
  15. Parker I., Miledi R. Nonlinearity and facilitation in phosphoinositide signaling studied by the use of caged inositol trisphosphate in Xenopus oocytes. J Neurosci. 1989 Nov;9(11):4068–4077. doi: 10.1523/JNEUROSCI.09-11-04068.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Rana R. S., Hokin L. E. Role of phosphoinositides in transmembrane signaling. Physiol Rev. 1990 Jan;70(1):115–164. doi: 10.1152/physrev.1990.70.1.115. [DOI] [PubMed] [Google Scholar]
  17. Seiffert U. B., Agranoff B. W. Isolation and separation of inositol phosphates from hydrolysates of rat tissues. Biochim Biophys Acta. 1965 Jun 1;98(3):574–581. doi: 10.1016/0005-2760(65)90154-2. [DOI] [PubMed] [Google Scholar]
  18. Shears S. B. Inositol phosphate metabolism: further problems and some solutions. Cell Signal. 1989;1(2):125–133. doi: 10.1016/0898-6568(89)90001-6. [DOI] [PubMed] [Google Scholar]
  19. Shears S. B. The pathway of myo-inositol 1,3,4-trisphosphate phosphorylation in liver. Identification of myo-inositol 1,3,4-trisphosphate 6-kinase, myo-inositol 1,3,4-trisphosphate 5-kinase, and myo-inositol 1,3,4,6-tetrakisphosphate 5-kinase. J Biol Chem. 1989 Nov 25;264(33):19879–19886. [PubMed] [Google Scholar]
  20. Snyder P. M., Krause K. H., Welsh M. J. Inositol trisphosphate isomers, but not inositol 1,3,4,5-tetrakisphosphate, induce calcium influx in Xenopus laevis oocytes. J Biol Chem. 1988 Aug 15;263(23):11048–11051. [PubMed] [Google Scholar]
  21. Stephens L. R., Downes C. P. Product-precursor relationships amongst inositol polyphosphates. Incorporation of [32P]Pi into myo-inositol 1,3,4,6-tetrakisphosphate, myo-inositol 1,3,4,5-tetrakisphosphate, myo-inositol 3,4,5,6-tetrakisphosphate and myo-inositol 1,3,4,5,6-pentakisphosphate in intact avian erythrocytes. Biochem J. 1990 Jan 15;265(2):435–452. doi: 10.1042/bj2650435. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Stephens L. R., Irvine R. F. Stepwise phosphorylation of myo-inositol leading to myo-inositol hexakisphosphate in Dictyostelium. Nature. 1990 Aug 9;346(6284):580–583. doi: 10.1038/346580a0. [DOI] [PubMed] [Google Scholar]
  23. Stith B. J., Proctor W. R. Microinjection of inositol 1,2-(cyclic)-4,5-trisphosphate, inositol 1,3,4,5-tetrakisphosphate, and inositol 1,4,5-trisphosphate into intact Xenopus oocytes can induce membrane currents independent of extracellular calcium. J Cell Biochem. 1989 Jul;40(3):321–330. doi: 10.1002/jcb.240400308. [DOI] [PubMed] [Google Scholar]

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