Skip to main content
PMC home page
Biochemical Journal logoLink to Biochemical Journal
. 1991 Jan 1;273(Pt 1):225–228. doi: 10.1042/bj2730225

Sequence of the beta-subunit of the phosphatidylinositol-specific phospholipase C-directed GTP-binding protein from squid (Loligo forbesi) photoreceptors.

N J Ryba 1, J D Pottinger 1, J N Keen 1, J B Findlay 1
PMCID: PMC1149903  PMID: 1899186

Abstract

The beta-subunit (G-beta) of the squid (Loligo forbesi) visual GTP-binding protein (G-protein), thought to be associated with a phosphatidylinositol-specific phospholipase C, has been identified and the sequence of the protein determined from its cDNA. The predicted polypeptide has a very marked sequence similarity with its mammalian counterparts (80-90% identity). Squid G-beta also has somewhat lower similarity to the yeast protein STE4 (approx. 40% identity). The role of G-beta in signal transduction is discussed in the light of its pronounced structural conservation.

Full text

PDF
228

Images in this article

226Fig. 1.
on p.226

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aviv H., Leder P. Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose. Proc Natl Acad Sci U S A. 1972 Jun;69(6):1408–1412. doi: 10.1073/pnas.69.6.1408. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Baer K. M., Saibil H. R. Light- and GTP-activated hydrolysis of phosphatidylinositol bisphosphate in squid photoreceptor membranes. J Biol Chem. 1988 Jan 5;263(1):17–20. [PubMed] [Google Scholar]
  3. Bloomquist B. T., Shortridge R. D., Schneuwly S., Perdew M., Montell C., Steller H., Rubin G., Pak W. L. Isolation of a putative phospholipase C gene of Drosophila, norpA, and its role in phototransduction. Cell. 1988 Aug 26;54(5):723–733. doi: 10.1016/s0092-8674(88)80017-5. [DOI] [PubMed] [Google Scholar]
  4. Bourne H. R. G-protein subunits. Who carries what message? Nature. 1989 Feb 9;337(6207):504–504. doi: 10.1038/337504a0. [DOI] [PubMed] [Google Scholar]
  5. Brett M., Findlay J. B. Isolation and characterization of the CNBr peptides from the proteolytically derived N-terminal fragment of ovine opsin. Biochem J. 1983 Jun 1;211(3):661–670. doi: 10.1042/bj2110661. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Casey P. J., Gilman A. G. G protein involvement in receptor-effector coupling. J Biol Chem. 1988 Feb 25;263(6):2577–2580. [PubMed] [Google Scholar]
  7. Chirgwin J. M., Przybyla A. E., MacDonald R. J., Rutter W. J. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry. 1979 Nov 27;18(24):5294–5299. doi: 10.1021/bi00591a005. [DOI] [PubMed] [Google Scholar]
  8. Dayhoff M. O., Schwartz R. M. Evidence on the origin of eukaryotic mitochondria from protein and nucleic acid sequences. Ann N Y Acad Sci. 1981;361:92–104. doi: 10.1111/j.1749-6632.1981.tb46513.x. [DOI] [PubMed] [Google Scholar]
  9. Fein A. Blockade of visual excitation and adaptation in Limulus photoreceptor by GDP-beta-S. Science. 1986 Jun 20;232(4757):1543–1545. doi: 10.1126/science.3487116. [DOI] [PubMed] [Google Scholar]
  10. Fong H. K., Amatruda T. T., 3rd, Birren B. W., Simon M. I. Distinct forms of the beta subunit of GTP-binding regulatory proteins identified by molecular cloning. Proc Natl Acad Sci U S A. 1987 Jun;84(11):3792–3796. doi: 10.1073/pnas.84.11.3792. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Gubler U., Hoffman B. J. A simple and very efficient method for generating cDNA libraries. Gene. 1983 Nov;25(2-3):263–269. doi: 10.1016/0378-1119(83)90230-5. [DOI] [PubMed] [Google Scholar]
  12. Kim D., Lewis D. L., Graziadei L., Neer E. J., Bar-Sagi D., Clapham D. E. G-protein beta gamma-subunits activate the cardiac muscarinic K+-channel via phospholipase A2. Nature. 1989 Feb 9;337(6207):557–560. doi: 10.1038/337557a0. [DOI] [PubMed] [Google Scholar]
  13. Levine M. A., Smallwood P. M., Moen P. T., Jr, Helman L. J., Ahn T. G. Molecular cloning of beta 3 subunit, a third form of the G protein beta-subunit polypeptide. Proc Natl Acad Sci U S A. 1990 Mar;87(6):2329–2333. doi: 10.1073/pnas.87.6.2329. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Marbach I., Bar-Sinai A., Minich M., Levitzki A. Beta subunit copurifies with GppNHp-activated adenylyl cyclase. J Biol Chem. 1990 Jun 15;265(17):9999–10004. [PubMed] [Google Scholar]
  15. Mumby S. M., Kahn R. A., Manning D. R., Gilman A. G. Antisera of designed specificity for subunits of guanine nucleotide-binding regulatory proteins. Proc Natl Acad Sci U S A. 1986 Jan;83(2):265–269. doi: 10.1073/pnas.83.2.265. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Neer E. J., Clapham D. E. Roles of G protein subunits in transmembrane signalling. Nature. 1988 May 12;333(6169):129–134. doi: 10.1038/333129a0. [DOI] [PubMed] [Google Scholar]
  17. O'Tousa J. E., Baehr W., Martin R. L., Hirsh J., Pak W. L., Applebury M. L. The Drosophila ninaE gene encodes an opsin. Cell. 1985 Apr;40(4):839–850. doi: 10.1016/0092-8674(85)90343-5. [DOI] [PubMed] [Google Scholar]
  18. Ovchinnikov YuA, Abdulaev N. G., Zolotarev A. S., Artamonov I. D., Bespalov I. A., Dergachev A. E., Tsuda M. Octopus rhodopsin. Amino acid sequence deduced from cDNA. FEBS Lett. 1988 May 9;232(1):69–72. doi: 10.1016/0014-5793(88)80388-0. [DOI] [PubMed] [Google Scholar]
  19. Robishaw J. D., Kalman V. K., Moomaw C. R., Slaughter C. A. Existence of two gamma subunits of the G proteins in brain. J Biol Chem. 1989 Sep 25;264(27):15758–15761. [PubMed] [Google Scholar]
  20. Roof D. J., Applebury M. L., Sternweis P. C. Relationships within the family of GTP-binding proteins isolated from bovine central nervous system. J Biol Chem. 1985 Dec 25;260(30):16242–16249. [PubMed] [Google Scholar]
  21. Stryer L., Bourne H. R. G proteins: a family of signal transducers. Annu Rev Cell Biol. 1986;2:391–419. doi: 10.1146/annurev.cb.02.110186.002135. [DOI] [PubMed] [Google Scholar]
  22. Sugimoto K., Nukada T., Tanabe T., Takahashi H., Noda M., Minamino N., Kangawa K., Matsuo H., Hirose T., Inayama S. Primary structure of the beta-subunit of bovine transducin deduced from the cDNA sequence. FEBS Lett. 1985 Oct 28;191(2):235–240. doi: 10.1016/0014-5793(85)80015-6. [DOI] [PubMed] [Google Scholar]
  23. Tabor S., Richardson C. C. DNA sequence analysis with a modified bacteriophage T7 DNA polymerase. Proc Natl Acad Sci U S A. 1987 Jul;84(14):4767–4771. doi: 10.1073/pnas.84.14.4767. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Uhl R., Wagner R., Ryba N. Watching G proteins at work. Trends Neurosci. 1990 Feb;13(2):64–70. doi: 10.1016/0166-2236(90)90070-q. [DOI] [PubMed] [Google Scholar]
  25. Vandenberg C. A., Montal M. Light-regulated biochemical events in invertebrate photoreceptors. 2. Light-regulated phosphorylation of rhodopsin and phosphoinositides in squid photoreceptor membranes. Biochemistry. 1984 May 22;23(11):2347–2352. doi: 10.1021/bi00306a004. [DOI] [PubMed] [Google Scholar]
  26. Wensel T. G., Stryer L. Activation mechanism of retinal rod cyclic GMP phosphodiesterase probed by fluorescein-labeled inhibitory subunit. Biochemistry. 1990 Feb 27;29(8):2155–2161. doi: 10.1021/bi00460a028. [DOI] [PubMed] [Google Scholar]
  27. Whiteway M., Hougan L., Dignard D., Thomas D. Y., Bell L., Saari G. C., Grant F. J., O'Hara P., MacKay V. L. The STE4 and STE18 genes of yeast encode potential beta and gamma subunits of the mating factor receptor-coupled G protein. Cell. 1989 Feb 10;56(3):467–477. doi: 10.1016/0092-8674(89)90249-3. [DOI] [PubMed] [Google Scholar]
  28. Yarfitz S., Provost N. M., Hurley J. B. Cloning of a Drosophila melanogaster guanine nucleotide regulatory protein beta-subunit gene and characterization of its expression during development. Proc Natl Acad Sci U S A. 1988 Oct;85(19):7134–7138. doi: 10.1073/pnas.85.19.7134. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES